Results 161 to 170 of about 159,475 (252)

Evolution‐guided yeast complementation reveals functional differences in human PSPH variants

FEBS Open Bio, EarlyView.
Ancient genomes can help guide which human genetic variants are tested experimentally. This study applies that idea to PSPH, a gene involved in serine biosynthesis, and uses high‐throughput yeast complementation to compare variant function. The findings reveal measurable differences among selected alleles and illustrate the value of evolution‐guided ...
Mauricio Campa‐Álvarez, Diana Ascencio, Miguel Vallebueno‐Estrada, Eduardo González‐Orozco, Christian Eduardo Martínez‐Guerrero, Rafael Montiel, Alexander DeLuna   +6 more
wiley   +1 more source

Pattern recognition and spatial differentiation of built environment around urban sports facilities from perspective of facility hierarchy: a case study of Changsha city. [PDF]

Front Public Health
Zhang G, Qiu S, Ding G.
europepmc   +1 more source

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

FEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty, Kelsi R. Hall, Zachary D. Tillett, Liam S. Turk, James S. Davies, Michael J. Currie, Hamish G. Brown, Sepideh Valimehr, Peter D. Mace, Andrew E. Whitten, Santosh Panjikar, Eric Hanssen, Rachel A. North, Renwick C.J. Dobson   +13 more
wiley   +1 more source

Travel Time to Healthcare and Type 2 Diabetes Mellitus Management in Korea: A Nationwide Retrospective Study. [PDF]

J Korean Med Sci
Ha R, Kim CY, Hwang SS, Jung-Choi K.
europepmc   +1 more source

kNN Search Algorithm with Index of the Minimum Road Travel Time in Time-Dependent Road Networks.

Inf. Media Technol., 2015
KOMAI, Yuka, NGUYEN, Duong Hong, HARA, Takahiro, NISHIO, Shojiro   +3 more
openaire   +2 more sources

In silico and in vitro exploration of a tyrosinase for biocatalytic production of catechols

FEBS Open Bio, EarlyView.
Tyrosinase from Ralstonia pseudosolanacearum is a promising biocatalyst for producing valuable catechols from monophenol substrates. This tyrosinase is uniquely suited to this due to its high monophenolase : diphenolase ratio. We combined in silico docking and in vivo kinetic characterisation of this tyrosinase with 11 industrially relevant monophenols,
James Britton, Fang Zhao, Reeta Davis, Meg Walsh, Kevin E O'Connor, David O'Connell, Tanja Narancic   +6 more
wiley   +1 more source

Genomic and epidemiological evidence linking a pre-season dengue case to local overwintering transmission in Guangdong, China. [PDF]

PLoS Negl Trop Dis
Lin L, Liu M, Chen J, Guan Q, Jiang S, Tan X, Zhang X, Jia P, Duan J, Deng H, Chen Q, Kang M, Li Y, He Y, Yang Z, Zhang M.   +15 more
europepmc   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Multiple sclerosis outcomes after cancer diagnosis in people with recorded chemotherapy exposure: A real-world MSBase study. [PDF]

Mult Scler
Nesbitt C, Sanfilippo P, Zhu C, Ozakbas S, Prat A, Girard M, Duquette P, Kalincik T, Roos I, Buzzard K, Skibina O, Foschi M, Surcinelli A, Lechner-Scott J, Patti F, Kermode AG, Fabis-Pedrini M, Carroll WM, Hodgkinson S, Grand'Maison F, Havrdova EK, Hradilek P, Habek M, Maimone D, Alroughani R, Vachova M, van Pesch V, Macdonell R, Spitaleri D, Khoury SJ, Gerlach O, de Gans K, McCombe P, Van Der Walt A, Butzkueven H, Jokubaitis V.   +35 more
europepmc   +1 more source

GelMA‐based 3D spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma

FEBS Open Bio, EarlyView.
GelMA 5% hydrogels support the formation of myeloid leukemia spheroids that recapitulate MS‐specific features, including G1 arrest, apoptosis, and ECM‐driven transcriptomic reprogramming. The 3D model mimicked soft‐tissue‐like stiffness and oxygen conditions, and transcriptomic convergence with primary MS samples confirmed its utility as a preclinical ...
Nicolas Germain, Elise Buret, Le Hoang Thanh Nguyen, Marion Cocheteux, Nicolas Touya, Mélanie Dhayer, Romain Dubois, Frank Lafont, Sebastien Janel, Patrice Maboudou, Steve Lancel, Philippe Marchetti   +11 more
wiley   +1 more source