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Treponema, Iron and Neurodegeneration
Current Alzheimer Research, 2018Spirochetes are suspected to be linked to the genesis of neurological diseases, including neurosyphillis or neurodegeneration (ND). Impaired iron homeostasis has been implicated in loss of function in several enzymes requiring iron as a cofactor, formation of toxic oxidative species, inflammation and elevated production of beta-amyloid proteins. This
Jolivet-Gougeon, A., Bonnaure-Mallet, M.
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Albumin requirement of Treponema denticola and Treponema vincentii
Canadian Journal of Microbiology, 1983Treponema denticola and Treponema vincentii were found to require albumin, oleic acid, and thiamine pyrophosphate (TPP) for growth. Previous studies indicated that commercial human alpha globulin, which is 50% albumin, was the only serum fraction that supported growth of these two oral treponemes.
K G, Van Horn, R M, Smibert
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Identification of Glycosylated Protein Antigens of Treponema pallidum and Treponema phagedenis
Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology, 1985Comparison of autoradiographies of intrinsically [35S] methionine and [14C] glucosamine labeled Treponema pallidum (Nichols strain) after sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) revealed four glycosylated proteins with molecular weights 30,500, 33,000, 35,000, and 59,000. T.
M, Moskophidis, F, Müller
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Fatty acid requirement of Treponema denticola and Treponema vincentii
Canadian Journal of Microbiology, 1982Treponema denticola and Treponema vincentii were cultured in a medium supplemented with either 0.2 or 0.4% (w/v) alpha globulin in place of serum. The active factor(s) in alpha globulin was stable at pH 7.0 to autoclaving and was nondialyzable. Extraction of lipids from alpha globulin showed that both protein and lipid, supplied by the alpha globulin,
K G, Van Horn, R M, Smibert
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1994
Publisher Summary This chapter discusses the culture of Treponerna pallidum ( T. pallidum ). T. pallidum can be cultivated in a variety of tissue culture vessels; 24-, 12-, and 6-well culture plates, and 25-, 75-, and 150-cm 2 tissue culture flasks, have been used successfully to cultivate T. pallidum .
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Publisher Summary This chapter discusses the culture of Treponerna pallidum ( T. pallidum ). T. pallidum can be cultivated in a variety of tissue culture vessels; 24-, 12-, and 6-well culture plates, and 25-, 75-, and 150-cm 2 tissue culture flasks, have been used successfully to cultivate T. pallidum .
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