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Laboratory Maintenance of Treponema denticola
Current Protocols in Microbiology, 2006AbstractThis unit describes the methods, media, and equipment necessary for routine laboratory culture and handling of the anaerobic oral spirochete Treponema denticola. Topics discussed include nutrient requirements, recommended media formulations, and expected growth kinetics, as well as methods and equipment necessary to maintain anaerobic ...
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Cytotoxic effect of peptidoglycan from Treponema denticola
Microbial Pathogenesis, 1993Peptidoglycan isolated from pathogenic bacteria has been previously found to exhibit various biological activities. The aim of this investigation was to evaluate the toxicity of Treponema denticola peptidoglycan towards epithelial cells. The cytotoxicity of a lipopolysaccharide-like material was also determined. Epithelial cells were incubated with the
D, Grenier, V J, Uitto
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Adherence of Treponema denticola to Modified Hydroxyapatite
Journal of Dental Research, 1987Small samples of hydroxyapatite (HA) beads were coated with 10 μL of either saliva, serum, human crevicular fluid, or a preparation of lysosomal enzymes from human polymorphonuclear leucocytes (PMN), before being added to suspensions of Treponema denticola. The beads were then observed with the scanning electron microscope. Abundant T. denticola were
G, Cimasoni, B C, McBride
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Antigenic and Structural Analysis of Treponema denticola
Microbiology, 1989Polypeptide and Western immunoblot profiles of subcellular fractions of Treponema denticola ATCC 33520 have been determined by SDS-PAGE of Triton X-100-soluble and -insoluble fractions, a lipopolysaccharide-enriched fraction and purified flagella. Major Triton X-100-soluble polypeptides of 72, 68, 54 and 52 kDa were detected.
A, Cockayne +6 more
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Fatty acid requirement of Treponema denticola and Treponema vincentii
Canadian Journal of Microbiology, 1982Treponema denticola and Treponema vincentii were cultured in a medium supplemented with either 0.2 or 0.4% (w/v) alpha globulin in place of serum. The active factor(s) in alpha globulin was stable at pH 7.0 to autoclaving and was nondialyzable. Extraction of lipids from alpha globulin showed that both protein and lipid, supplied by the alpha globulin,
K G, Van Horn, R M, Smibert
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Development of quasi-multicellular bodies of Treponema denticola
Archives of Microbiology, 1993The formation of quasi-multicellular bodies of Treponema denticola was analysed using different electron microscopical methods. These bacteria could develop four different conformations: (i) normal helical forms; (ii) twisted spirochetes, forming plaits; (iii) twisted spirochetes,forming club-like structures; (iv) spherical bodies in different size ...
V, Wolf, R, Lange, J, Wecke
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Amino acid and glucose fermentation by Treponema denticola
Archiv f�r Mikrobiologie, 1971Treponema denticola was grown in serum-containing media to which 14C-labelled compounds were added. Determinations of radioactivity in the products formed indicated that the organism fermented alanine, cysteine, glycine, serine, and glucose. Fermentation products included acetate, lactate, succinate, formate, pyruvate, ethanol, CO2, H2S, and NH3.
R B, Hespell, E, Canale-Parola
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Role of Treponema Denticola in Periodontal Diseases
Critical Reviews in Oral Biology & Medicine, 2001Among periodontal anaerobic pathogens, the oral spirochetes, and especially Treponema denticola, have been associated with periodontal diseases such as early-onset periodontitis, necrotizing ulcerative gingivitis, and acute pericoronitis. Basic research as well as clinical evidence suggest that the prevalence of T.
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Molecular Characterization of the Treponema denticola fliQ Region
DNA Sequence, 2001A Treponema denticola 4.2 kb DNA region containing four complete genes (orfl, fliQ, fliR, and flhB) and a truncated gene (flhA') was sequenced and analyzed. The deduced amino acid sequences of FliQ, FliR, FlhB and FlhA' have significant homology with bacterial proteins associated with the flagellar export apparatus, whereas the deduced amino acid ...
L V, Stamm, H L, Bergen
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Growth stimulation of Treponema denticola by periodontal microorganisms
Antonie van Leeuwenhoek, 1990Previous experiments have indicated that enrichment of subgingival plaque in human serum can lead to the accumulation of Treponema denticola. T. denticola depends on bacterial interactions for its growth in serum. Aim of the present study was to identify specific microorganisms involved in the growth stimulation of T. denticola.
P F, ter Steeg, J S, van der Hoeven
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