Results 201 to 210 of about 14,110 (240)

Genetic Manipulation of Treponema denticola

Current Protocols in Microbiology, 2006
AbstractThe oral anaerobic spirochete, Treponema denticola, has been implicated in the etiology of human periodontal diseases; however, the molecular basis for the virulence of these organisms is still unclear. Potential pathogenic factors expressed by T. denticola have recently begun to be identified through the development of gene transfer approaches
Howard K, Kuramitsu, Bo, Chi, Akihiko, Ikegami   +2 more
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Oxygen metabolism by Treponema denticola

Oral Microbiology and Immunology, 1999
Treponema denticola strains ATCC 35405 and ASLM were found to have moderately active oxygen metabolism and consumed some 0.46 μmol O2/h/mg cell protein in anaerobic growth medium or about ten times this amount in aerobic medium. There appeared to be no differences between the two strains in their oxidative metabolism.
C E, Caldwell, R E, Marquis
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A Study of the Acid Phosphatase of Treponema denticola

Zentralblatt für Bakteriologie, 1990
This study describes some of the properties of the acid phosphatase of the potential periodontopathogen Treponema denticola. The highest enzyme activity was found in 87 h old cells. Two optimum pHs for enzyme activity were detected, one at pH 4.8 and another at pH 6.2. Divalent cations did not influence the acid phosphatase of T. denticola. The anion F-
C A, Norton Hughes, W W, Yotis
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Adherence of Treponema denticola to Modified Hydroxyapatite

Journal of Dental Research, 1987
Small samples of hydroxyapatite (HA) beads were coated with 10 μL of either saliva, serum, human crevicular fluid, or a preparation of lysosomal enzymes from human polymorphonuclear leucocytes (PMN), before being added to suspensions of Treponema denticola. The beads were then observed with the scanning electron microscope. Abundant T. denticola were
G, Cimasoni, B C, McBride
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Antigenic and Structural Analysis of Treponema denticola

Microbiology, 1989
Polypeptide and Western immunoblot profiles of subcellular fractions of Treponema denticola ATCC 33520 have been determined by SDS-PAGE of Triton X-100-soluble and -insoluble fractions, a lipopolysaccharide-enriched fraction and purified flagella. Major Triton X-100-soluble polypeptides of 72, 68, 54 and 52 kDa were detected.
A, Cockayne, R, Sanger, A, Ivic, R A, Strugnell, J H, MacDougall, R R, Russell, C W, Penn   +6 more
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Role of Treponema Denticola in Periodontal Diseases

Critical Reviews in Oral Biology & Medicine, 2001
Among periodontal anaerobic pathogens, the oral spirochetes, and especially Treponema denticola, have been associated with periodontal diseases such as early-onset periodontitis, necrotizing ulcerative gingivitis, and acute pericoronitis. Basic research as well as clinical evidence suggest that the prevalence of T.
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Albumin requirement of Treponema denticola and Treponema vincentii

Canadian Journal of Microbiology, 1983
Treponema denticola and Treponema vincentii were found to require albumin, oleic acid, and thiamine pyrophosphate (TPP) for growth. Previous studies indicated that commercial human alpha globulin, which is 50% albumin, was the only serum fraction that supported growth of these two oral treponemes.
K G, Van Horn, R M, Smibert
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Immunochemical features of a macromolecule of Treponema denticola

Journal of Basic Microbiology, 1995
AbstractIn this study the extraction and the immunochemical features of a lipopolysaccharide‐like (LPSL) macromolecule of T. denticola strains 35405, 35404, 33521 and 11 were investigated. The yield of LPSL molecule ranged between 0.5‐0.9% of the cell dry weight, it possessed Limulus amebocyte lysate clotting activity, and it contained glucosamine ...
W W, Yotis, F, Macaluso, C, Gopalsami
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Laboratory Maintenance of Treponema denticola

Current Protocols in Microbiology, 2006
AbstractThis unit describes the methods, media, and equipment necessary for routine laboratory culture and handling of the anaerobic oral spirochete Treponema denticola. Topics discussed include nutrient requirements, recommended media formulations, and expected growth kinetics, as well as methods and equipment necessary to maintain anaerobic ...
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Cytotoxic effect of peptidoglycan from Treponema denticola

Microbial Pathogenesis, 1993
Peptidoglycan isolated from pathogenic bacteria has been previously found to exhibit various biological activities. The aim of this investigation was to evaluate the toxicity of Treponema denticola peptidoglycan towards epithelial cells. The cytotoxicity of a lipopolysaccharide-like material was also determined. Epithelial cells were incubated with the
D, Grenier, V J, Uitto
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