High-purity DNA extraction from animal tissue using picking in the TRIzol-based method
BioTechniques, 2021 TRIzol is used for the extraction of RNA, DNA and proteins from tissues or cells. Here, we present a simple picking method to extract DNA from tissues using TRIzol.
Ye Yin Bo +6 more
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TRIzol-based RNA extraction for detection protocol for SARS-CoV-2 of coronavirus disease 2019
New Microbes and New Infections, 2021 Diagnostic testing is important for managing the 2019 novel coronavirus (SARS-CoV-2). We developed an optimized protocol for SARS-CoV-2 RNA extraction from the surface of the respiratory mucosa with nasopharyngeal swabs and compared the sensitivity of ...
A. Amirouche +6 more
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Comparison of Five TRIzol-Based Protein Preparation Methods for 2-DE Production From Challenging Marine Dinoflagellate Samples: A Case Study on Two Benthic Prorocentrum Species [PDF]
Journal of Marine Science and Engineering, 2020 Two-dimensional gel electrophoresis (2-DE) is a major element of conventional gel-based proteomics, which resolves complex protein mixtures. Protein extraction with the removal of interfering substances from the sample remains the key to producing high ...
Thomas Chun-Hung Lee +4 more
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Journal of Extracellular Vesicles, 2018 Sample amount is often a limiting factor for multi-parametric analyses that encompass at least three areas of ‘-omics’ research: genomics, transcriptomics and proteomics.
Andrew P. Joy +8 more
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High-yield skeletal muscle protein recovery from TRIzol after RNA and DNA extraction
BioTechniques, 2020 Extraction of DNA, RNA and protein from the same sample would allow for direct comparison of genomic, transcriptomic and proteomic information. Commercially available kits exhibit poor protein yield and the TRIzol® reagent produces a protein pellet that ...
Yuan Wen +3 more
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Automated RNA Purification directly from TRIzol® on the Hamilton Microlab® STAR™ [PDF]
BioTechniques, 2018 The TRIzol method for RNA extraction has been the gold standard. The powerful protein denaturant effectively stabilizes RNA and inactivates RNases and infectious agents.
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Stabilising the Integrity of Snake Venom mRNA Stored under Tropical Field Conditions Expands Research Horizons. [PDF]
PLoS Neglected Tropical Diseases, 2016 BackgroundSnake venoms contain many proteinaceous toxins that can cause severe pathology and mortality in snakebite victims. Interestingly, mRNA encoding such toxins can be recovered directly from venom, although yields are low and quality is unknown. It
Gareth Whiteley +8 more
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Optimising the Use of TRIzol-extracted Proteins in Surface Enhanced Laser Desorption/ Ionization (SELDI) Analysis [PDF]
Proteome Science, 2006 Background Research with clinical specimens is always hampered by the limited availability of relevant samples, necessitating the use of a single sample for multiple assays. TRIzol is a common reagent for RNA extraction, but DNA and protein fractions can
Perlaky Laszlo +5 more
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Optimizing total RNA extraction method for human and mice samples [PDF]
PeerJBackground Extracting high-quality total RNA is pivotal for advanced RNA molecular studies, such as Next-generation sequencing and expression microarrays where RNA is hybridized.
Yumei Zeng +4 more
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TRIzol treatment of secretory phase endometrium allows combined proteomic and mRNA microarray analysis of the same sample in women with and without endometriosis [PDF]
Reproductive Biology and Endocrinology, 2010 Background According to mRNA microarray, proteomics and other studies, biological abnormalities of eutopic endometrium (EM) are involved in the pathogenesis of endometriosis, but the relationship between mRNA and protein expression in EM is not clear. We
Gevaert Olivier +9 more
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