Comparison of Five TRIzol-Based Protein Preparation Methods for 2-DE Production From Challenging Marine Dinoflagellate Samples: A Case Study on Two Benthic Prorocentrum Species [PDF]
Journal of Marine Science and Engineering, 2020 Two-dimensional gel electrophoresis (2-DE) is a major element of conventional gel-based proteomics, which resolves complex protein mixtures. Protein extraction with the removal of interfering substances from the sample remains the key to producing high ...
Thomas Chun-Hung Lee +4 more
doaj +3 more sources
TRIzol-based RNA extraction for detection protocol for SARS-CoV-2 of coronavirus disease 2019
New Microbes and New Infections, 2021 Diagnostic testing is important for managing the 2019 novel coronavirus (SARS-CoV-2). We developed an optimized protocol for SARS-CoV-2 RNA extraction from the surface of the respiratory mucosa with nasopharyngeal swabs and compared the sensitivity of ...
A. Amirouche +6 more
doaj +3 more sources
Effectiveness of TRIzol in Inactivating Animal Pathogens [PDF]
Applied Biosafety, 2023 Safe handling of biological samples sourced from wild ecosystems is a pressing concern for scientists in disparate fields, including ecology and evolution, OneHealth initiatives, bioresources, geography, veterinary medicine, conservation, and many others. This is especially relevant given the growing global research community and collaborative networks
Gwen Duytschaever +4 more
semanticscholar +4 more sources
Proteome profiling of extracellular vesicles captured with the affinity peptide Vn96: comparison of Laemmli and TRIzol© protein-extraction methods [PDF]
Journal of Extracellular Vesicles, 2018 Sample amount is often a limiting factor for multi-parametric analyses that encompass at least three areas of ‘-omics’ research: genomics, transcriptomics and proteomics.
Andrew P. Joy +8 more
doaj +3 more sources
Efficient recovery of proteins from multiple source samples after trizol® or trizol®LS RNA extraction and long-term storage [PDF]
BMC Genomics, 2013 Abstract Background Simultaneous isolation of nucleic acids and proteins from a single biological sample facilitates meaningful data interpretation and reduces time, cost and sampling errors. This is particularly relevant for rare human and animal specimens, often scarce, and/or irreplaceable.
André E. Simões +11 more
semanticscholar +5 more sources
Stabilising the Integrity of Snake Venom mRNA Stored under Tropical Field Conditions Expands Research Horizons. [PDF]
PLoS Neglected Tropical Diseases, 2016 BackgroundSnake venoms contain many proteinaceous toxins that can cause severe pathology and mortality in snakebite victims. Interestingly, mRNA encoding such toxins can be recovered directly from venom, although yields are low and quality is unknown. It
Gareth Whiteley +8 more
doaj +3 more sources
Optimising the Use of TRIzol-extracted Proteins in Surface Enhanced Laser Desorption/ Ionization (SELDI) Analysis [PDF]
Proteome Science, 2006 Background Research with clinical specimens is always hampered by the limited availability of relevant samples, necessitating the use of a single sample for multiple assays. TRIzol is a common reagent for RNA extraction, but DNA and protein fractions can
Perlaky Laszlo +5 more
doaj +2 more sources
RNA extraction protocol (Trizol) v1 [PDF]
, 2016 This protocol describes how to extract total RNA from flatworms. It is from: Hebert, F, O; Grambauer, S; Barber, I; Landry, C, R; Aubin-Horth, N (2016): Reference transcriptome sequence resource for the study of the Cestode Schistocephalus solidus, a threespine stickleback parasite. GigaScience Database. http://dx.doi.org/10.5524/100197
Hebert F.O. +4 more
openalex +2 more sources
RNA Extraction with Trizol v1 [PDF]
, 2020 This is a protocol for RNA extractions (using Trizol). A maximum of 3 samples can be extracted at once using this protocol. The entire protocol should be run in the fumehood. This protocol is time- and temperature-sensitive, so read all instructions before attempting and make sure you are adequately prepared.
Dakota Betz
openalex +2 more sources