High-purity DNA extraction from animal tissue using picking in the TRIzol-based method
BioTechniques, 2021 TRIzol is used for the extraction of RNA, DNA and proteins from tissues or cells. Here, we present a simple picking method to extract DNA from tissues using TRIzol.
Ye Yin Bo +6 more
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TRIzol-based RNA extraction for detection protocol for SARS-CoV-2 of coronavirus disease 2019
New Microbes and New Infections, 2021 Diagnostic testing is important for managing the 2019 novel coronavirus (SARS-CoV-2). We developed an optimized protocol for SARS-CoV-2 RNA extraction from the surface of the respiratory mucosa with nasopharyngeal swabs and compared the sensitivity of ...
A. Amirouche +6 more
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Automated RNA Purification directly from TRIzol® on the Hamilton Microlab® STAR™ [PDF]
BioTechniques, 2018 The TRIzol method for RNA extraction has been the gold standard. The powerful protein denaturant effectively stabilizes RNA and inactivates RNases and infectious agents.
doaj +3 more sources
Comparison of Five TRIzol-Based Protein Preparation Methods for 2-DE Production From Challenging Marine Dinoflagellate Samples: A Case Study on Two Benthic Prorocentrum Species [PDF]
Journal of Marine Science and Engineering, 2020 Two-dimensional gel electrophoresis (2-DE) is a major element of conventional gel-based proteomics, which resolves complex protein mixtures. Protein extraction with the removal of interfering substances from the sample remains the key to producing high ...
Thomas Chun-Hung Lee +4 more
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Genomic RNA extraction by TRIzol method by using Drosophila melanogastergut v1 [PDF]
, 2022 Simple method followed for RNA extraction of Drosophila melanogaster larval(3rd instar) gut for CDNA
Mohamed Sabeelil Islam
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Stabilising the Integrity of Snake Venom mRNA Stored under Tropical Field Conditions Expands Research Horizons. [PDF]
PLoS Neglected Tropical Diseases, 2016 BackgroundSnake venoms contain many proteinaceous toxins that can cause severe pathology and mortality in snakebite victims. Interestingly, mRNA encoding such toxins can be recovered directly from venom, although yields are low and quality is unknown. It
Gareth Whiteley +8 more
doaj +3 more sources
RNA Extraction with Trizol for cells v2 [PDF]
, 2018 Maria Cármen Sales +2 more
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Optimising the Use of TRIzol-extracted Proteins in Surface Enhanced Laser Desorption/ Ionization (SELDI) Analysis [PDF]
Proteome Science, 2006 Background Research with clinical specimens is always hampered by the limited availability of relevant samples, necessitating the use of a single sample for multiple assays. TRIzol is a common reagent for RNA extraction, but DNA and protein fractions can
Perlaky Laszlo +5 more
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TRIzol and Alu qPCR-based quantification of metastatic seeding within the skeleton [PDF]
Scientific Reports, 2015 AbstractCurrent methods for detecting disseminated tumor cells in the skeleton are limited by expense and technical complexity. We describe a simple and inexpensive method to quantify, with single cell sensitivity, human metastatic cancer in the mouse skeleton, concurrently with host gene expression, using TRIzol-based DNA/RNA extraction and Alu ...
James Campbell +8 more
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An Optimised TRIzol-based Protocol for the Improvement of RNA Extraction Yield of Tomato Stem [PDF]
Pertanika Journal of Tropical Agricultural Science, 2021 One of the most common methods for purifying RNA is using TRIzol reagent because of its simplicity and economic feasibility. However, the drawback of this method is frequently the low quality of extracted RNA due to contaminants from the residue of phenol and guanidinium thiocyanate from the reagents. This study aimed to evaluate the improvement in the
Anis Afifah +5 more
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