Results 91 to 100 of about 452,059 (305)

Isolation of functional total RNA from Tilia cordata leaves and pollen [PDF]

open access: yesJournal of the Serbian Chemical Society, 2012
Conditions for isolation of high quality total RNA from European linden (Tilia cordata) leaves and pollen were determined. Pure total RNA was isolated from linden leaves utilizing Qiagen plant mini kit while total RNA isolated from linden pollen using
Ognjenović Jana   +4 more
doaj   +1 more source

Methylation of the Gpat2 promoter regulates transient expression during mouse spermatogenesis [PDF]

open access: yes, 2015
Spermatogenesis is a highly regulated process that involves both mitotic and meiotic divisions, as well as cellular differentiation to yield mature spermatozoa from undifferentiated germinal stem cells.
Cattaneo, Elizabeth Renee   +6 more
core   +2 more sources

LIN28B Promotes Cancer Cell Dissemination and Angiogenesis

open access: yesAdvanced Biology, EarlyView.
Children diagnosed with high‐risk neuroblastoma have a 5‐year event‐free survival rate of less than 50% and poor outcomes after recurrence. Deregulation of the LIN28B oncogene can be addressed in these patients. Upregulation of LIN28B is shown to support the metastatic cascade.
Diana Corallo   +8 more
wiley   +1 more source

Assay reproducibility in clinical studies of plasma miRNA. [PDF]

open access: yesPLoS ONE, 2015
There are increasing reports of plasma miRNAs as biomarkers of human disease but few standards in methodologic reporting, leading to inconsistent data.
Jonathan Rice   +6 more
doaj   +1 more source

Downregulation of 26S proteasome catalytic activity promotes epithelial-mesenchymal transition. [PDF]

open access: yes, 2016
The epithelial-mesenchymal transition (EMT) endows carcinoma cells with phenotypic plasticity that can facilitate the formation of cancer stem cells (CSCs) and contribute to the metastatic cascade.
Banno, Asoka   +15 more
core   +2 more sources

Adipose Mesenchymal Stem Cell‐Derived Exosomes in Conjunction with Roflumilast Ameliorate Chronic Kidney Disease Through the Modulation of Fibrosis and Inflammation

open access: yesAdvanced Biology, EarlyView.
The novelty of this study showed that the injection of exosomes produced from ADMSCs in combination with Roflumilast poses a more favorable therapeutic outcome for CKD induced by Adriamycin, compared to therapy with exosomes or Roflumilast alone. Roflumilast and exosomes treatment lowered the expression of the apoptotic, fibrotic, and inflammatory ...
Mohamed Ali   +5 more
wiley   +1 more source

Pitfalls of DNA Quantification Using DNA-Binding Fluorescent Dyes and Suggested Solutions.

open access: yesPLoS ONE, 2016
The Qubit fluorometer is a DNA quantification device based on the fluorescence intensity of fluorescent dye binding to double-stranded DNA (dsDNA). Qubit is generally considered useful for checking DNA quality before next-generation sequencing because it
Yuki Nakayama   +3 more
doaj   +1 more source

An efficient method for total RNA extraction from leaves of arboreal species from the Brazilian Cerrado

open access: yesRodriguésia, 2020
Considering the lack of information on RNA extraction from arboreal species, specially from the Brazilian Cerrado, the aim of this study was to test RNA extraction methods for a wide variety of native plant species from this biome.
Geisiane Alves Rocha   +4 more
doaj   +1 more source

Expression of functional recombinant human tissue transglutaminase (TG2) using the bac-to-bac baculovirus expression system [PDF]

open access: yes, 2016
Purpose: Tissue transglutaminase (TG2) is a unique multifunctional enzyme. The enzyme possesses enzymatic activities such as transamidation/crosslinking and non-enzymatic functions such as cell migration and signal transduction.
Azari, S., Kalhor, H.R., Yazdani, Y.
core   +2 more sources

Loss of Stat3 in Prx1+ Progenitors Impairs Molar Root Development

open access: yesAdvanced Biology, EarlyView.
Stat3 critically regulates mandibular first molar and alveolar bone morphogenesis. Conditional ablation of Stat3 disrupts the osteogenic capacity of Prx1+ mesenchymal progenitors, as evidenced across in vivo and in vitro models. Abstract Signal Transducer and Activator of Transcription 3 (Stat3) acts as a central transcriptional modulator coordinating ...
Xin Feng   +10 more
wiley   +1 more source

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