Results 91 to 100 of about 463,109 (314)

Increased Expression of Cell-Cell Signaling Genes by Stimulated Mononuclear Leukocytes in Patients with Previous Atherothrombotic Stroke A Whole Genome Expression Profile Study [PDF]

, 2009
Background/Aims: Inflammation plays an important role in atherosclerosis and stroke. Acute infections are recognized as trigger factors for ischemic stroke. Methods: In this whole genome expression profile study of 15 patients and 15 control subjects, we
Grau, Armin J., Grond-Ginsbach, Caspar, Hergenhahn, Manfred, Hollstein, Monika, Honold, Cornelius, Horstmann, Solveig, Hummel, Manuela, Knyazev, Yuri, Mansmann, Ulrich, Pfleger, Karin, Wagner, Simone, Weninger, Annette, Wiest, Tina   +12 more
core   +1 more source

Immune‐Driven Expression in Inclusion Body Myositis With T‐Cell Large Granular Lymphocytic Leukemia

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objectives T‐cell large granular lymphocytic leukemia (T‐LGLL), reported in up to 58% of inclusion body myositis (IBM) patients, is a rare leukemia of cytotoxic or less commonly helper T cells. The range of myopathies in T‐LGLL and the impact of coexisting T‐LGLL in IBM are not well understood. Our objectives are to investigate the spectrum of
Pannathat Soontrapa, Iago Pinal‐Fernandez, Pritikanta Paul, Michael P. Skolka, Margherita Milone, Min Shi, Mithun V. Shah, Maria Casal‐Dominguez, Katherine Pak, Andrew L. Mammen, Teerin Liewluck   +10 more
wiley   +1 more source

Assay reproducibility in clinical studies of plasma miRNA. [PDF]

PLoS ONE, 2015
There are increasing reports of plasma miRNAs as biomarkers of human disease but few standards in methodologic reporting, leading to inconsistent data.
Jonathan Rice, Henry Roberts, James Burton, Jianmin Pan, Vanessa States, Shesh N Rai, Susan Galandiuk   +6 more
doaj   +1 more source

Synergistic Osteogenesis After Co‐Administration of cmRNAs Encoding BMP‐2 and BMP‐7 Utilizing a Transcript‐Activated Matrix

Advanced Functional Materials, EarlyView.
This study demonstrates that the dual delivery of BMP‐2/‐7 coding cmRNAs for bone healing is demonstrated as feasible, safe, and highly osteogenic. Compared to single BMP‐2 or BMP‐7 cmRNAs, the combination enhances the production of both mineral and organic components of the extracellular matrix when delivered using a collagen‐HA scaffold, supporting ...
Claudia Del Toro Runzer, Joanna M. Sadowska, Nadia Roumans, Christian Plank, Christopher H. Evans, Fergal J. O'Brien, Martijn van Griensven, Elizabeth R. Balmayor   +7 more
wiley   +1 more source

Pitfalls of DNA Quantification Using DNA-Binding Fluorescent Dyes and Suggested Solutions.

PLoS ONE, 2016
The Qubit fluorometer is a DNA quantification device based on the fluorescence intensity of fluorescent dye binding to double-stranded DNA (dsDNA). Qubit is generally considered useful for checking DNA quality before next-generation sequencing because it
Yuki Nakayama, Hiromi Yamaguchi, Naoki Einaga, Mariko Esumi   +3 more
doaj   +1 more source

An efficient method for total RNA extraction from leaves of arboreal species from the Brazilian Cerrado

Rodriguésia, 2020
Considering the lack of information on RNA extraction from arboreal species, specially from the Brazilian Cerrado, the aim of this study was to test RNA extraction methods for a wide variety of native plant species from this biome.
Geisiane Alves Rocha, Vanessa Duarte Dias, Renato Carrer-Filho, Marcos Gomes da Cunha, Érico de Campos Dianese   +4 more
doaj   +1 more source

Enzyme‐Regulated Extended Swelling of Hydrogels for Dehiscence‐Less Tissue Expansions

Advanced Functional Materials, EarlyView.
An interpenetrating hydrogel network with swelling under regulation by enzymatic degradation (INSURED) is fabricated to avoid dehiscence. INSURED remains structurally intact post‐implantation, while HYAL injection enables control over the onset and rate of swelling.
Byung Ik Park, Dong‐yup Lee, Sungsoo Lim, JangHyuk Kim, Min‐Gyu Lee, Chang Seo Park, Hyeonuk Na, Yong Eun Cho, Yun Hyeok Lee, Junsang Doh, Jeong‐Yun Sun   +10 more
wiley   +1 more source

Effective DNA/RNA co-extraction for analysis of microRNAs, mRNAs, and genomic DNA from formalin-fixed paraffin-embedded specimens. [PDF]

PLoS ONE, 2012
BACKGROUND: Retrospective studies of archived human specimens, with known clinical follow-up, are used to identify predictive and prognostic molecular markers of disease.
Adam Kotorashvili, Andrew Ramnauth, Christina Liu, Juan Lin, Kenny Ye, Ryung Kim, Rachel Hazan, Thomas Rohan, Susan Fineberg, Olivier Loudig   +9 more
doaj   +1 more source

Identification of the alternative oxidase gene and its expression in the copepod Tigriopus californicus [PDF]

, 2019
In addition to the typical electron transport system (ETS) in animal mitochondria responsible for oxidative phosphorylation, in some species there exists an alternative oxidase (AOX) pathway capable of catalyzing the oxidation of ubiquinol and the ...
Cygelfarb, Willie, McDonald, Allison, Singh, Jaspreet, Tward, Carly E.   +3 more
core   +1 more source

TRIzol and Alu qPCR-based quantification of metastatic seeding within the skeleton [PDF]

Scientific Reports, 2015
AbstractCurrent methods for detecting disseminated tumor cells in the skeleton are limited by expense and technical complexity. We describe a simple and inexpensive method to quantify, with single cell sensitivity, human metastatic cancer in the mouse skeleton, concurrently with host gene expression, using TRIzol-based DNA/RNA extraction and Alu ...
J. Preston Campbell, P. Mulcrone, S. K. Masood, M. Karolak, A. Merkel, K. Hebron, A. Zijlstra, J. Sterling, F. Elefteriou   +8 more
openaire   +2 more sources