Assay reproducibility in clinical studies of plasma miRNA. [PDF]
PLoS ONE, 2015 There are increasing reports of plasma miRNAs as biomarkers of human disease but few standards in methodologic reporting, leading to inconsistent data.
Jonathan Rice +6 more
doaj +1 more source
Advanced Functional Materials, EarlyView.In this work, melt electrowriting is used to fabricate a 3D printed scaffold design that generates engineered cardiac tissues with in‐plane contraction, mimicking natural myocardium. It is shown that these tissues display advanced maturation and functionality.
Olalla Iglesias‐García +23 more
wiley +1 more source
MethodsX, 2020 A multitude of challenges is faced during RNA extraction from human visceral adipose tissue (VAT) due to its atypical nature and a dearth of existing literature.
Dipayan Roy +4 more
doaj
Pitfalls of DNA Quantification Using DNA-Binding Fluorescent Dyes and Suggested Solutions.
PLoS ONE, 2016 The Qubit fluorometer is a DNA quantification device based on the fluorescence intensity of fluorescent dye binding to double-stranded DNA (dsDNA). Qubit is generally considered useful for checking DNA quality before next-generation sequencing because it
Yuki Nakayama +3 more
doaj +1 more source
Rodriguésia, 2020 Considering the lack of information on RNA extraction from arboreal species, specially from the Brazilian Cerrado, the aim of this study was to test RNA extraction methods for a wide variety of native plant species from this biome.
Geisiane Alves Rocha +4 more
doaj +1 more source
Mycotoxins nivalenol and deoxynivalenol differently modulate cytokine mRNA expression in Jurkat T cells. [PDF]
, 2006 Deoxynivalenol (DON) and its hydroxylated form nivalenol (NIV) are Fusarium mycotoxins that occur in cereal grains alone or in combination. Several studies have shown that these metabolites affect lymphocyte functions.
BERGAMO P +4 more
core +1 more source
Electric Pulse Regulated MXene Based Nanozymes for Integrative Bioelectricity Immuno‐Cancer Therapy
Advanced Functional Materials, EarlyView.MXenzyme‐mediated bioelectricity cancer therapy (MXenzyme‐BECT) enhances cancer cell death through irreversible depolarization, ion channel disruption, ROS generation, and immunogenic cell death. Computational simulations reveal the electrical mechanisms by which MXenzyme acts on single cells and support to predict treatment parameters. Next‐generation
Sanghee Lee +6 more
wiley +1 more source
Effective DNA/RNA co-extraction for analysis of microRNAs, mRNAs, and genomic DNA from formalin-fixed paraffin-embedded specimens. [PDF]
PLoS ONE, 2012 BACKGROUND: Retrospective studies of archived human specimens, with known clinical follow-up, are used to identify predictive and prognostic molecular markers of disease.
Adam Kotorashvili +9 more
doaj +1 more source
MTR4 drives liver tumorigenesis by promoting cancer metabolic switch through alternative splicing. [PDF]
, 2020 The metabolic switch from oxidative phosphorylation to glycolysis is required for tumorigenesis in order to provide cancer cells with energy and substrates of biosynthesis.
Chen, Wancheng +16 more
core +1 more source
Single cell analysis reveals the involvement of the long non-coding RNA Pvt1 in the modulation of muscle atrophy and mitochondrial network [PDF]
, 2019 Long non-coding RNAs (lncRNAs) are emerging as important players in the regulation of several aspects of cellular biology. For a better comprehension of their function, it is fundamental to determine their tissue or cell specificity and to identify their
Alessio, Enrico +13 more
core +1 more source