Results 111 to 120 of about 461,258 (325)

Effect of Long-term Storage in TRIzol on Microarray-Based Gene Expression Profiling [PDF]

Cancer Epidemiology, Biomarkers & Prevention, 2010
Abstract Background: Although TRIzol is widely used for preservation and isolation of RNA, there is suspicion that prolonged sample storage in TRIzol may affect array-based gene expression profiling (GEP) through premature termination during reverse transcription.
Wencai, Ma, Michael, Wang, Zhi-Qiang, Wang, Luhong, Sun, David, Graber, Jairo, Matthews, Richard, Champlin, Qing, Yi, Robert Z, Orlowski, Larry W, Kwak, Donna M, Weber, Sheeba K, Thomas, Jatin, Shah, Steven, Kornblau, Richard E, Davis   +14 more
openaire   +2 more sources

Loss of Stat3 in Prx1+ Progenitors Impairs Molar Root Development

Advanced Biology, EarlyView.
Stat3 critically regulates mandibular first molar and alveolar bone morphogenesis. Conditional ablation of Stat3 disrupts the osteogenic capacity of Prx1+ mesenchymal progenitors, as evidenced across in vivo and in vitro models. Abstract Signal Transducer and Activator of Transcription 3 (Stat3) acts as a central transcriptional modulator coordinating ...
Xin Feng, Wangyu Luo, Yichen Yao, Lichieh Lin, Laiting Chan, Jiarui Lu, Zijing Huang, Jingyi Feng, Le Zhao, Xiaolei Zhang, Liu Yang   +10 more
wiley   +1 more source

Pitfalls of DNA Quantification Using DNA-Binding Fluorescent Dyes and Suggested Solutions.

PLoS ONE, 2016
The Qubit fluorometer is a DNA quantification device based on the fluorescence intensity of fluorescent dye binding to double-stranded DNA (dsDNA). Qubit is generally considered useful for checking DNA quality before next-generation sequencing because it
Yuki Nakayama, Hiromi Yamaguchi, Naoki Einaga, Mariko Esumi   +3 more
doaj   +1 more source

An efficient method for total RNA extraction from leaves of arboreal species from the Brazilian Cerrado

Rodriguésia, 2020
Considering the lack of information on RNA extraction from arboreal species, specially from the Brazilian Cerrado, the aim of this study was to test RNA extraction methods for a wide variety of native plant species from this biome.
Geisiane Alves Rocha, Vanessa Duarte Dias, Renato Carrer-Filho, Marcos Gomes da Cunha, Érico de Campos Dianese   +4 more
doaj   +1 more source

Increased Expression of Cell-Cell Signaling Genes by Stimulated Mononuclear Leukocytes in Patients with Previous Atherothrombotic Stroke A Whole Genome Expression Profile Study [PDF]

, 2009
Background/Aims: Inflammation plays an important role in atherosclerosis and stroke. Acute infections are recognized as trigger factors for ischemic stroke. Methods: In this whole genome expression profile study of 15 patients and 15 control subjects, we
Grau, Armin J., Grond-Ginsbach, Caspar, Hergenhahn, Manfred, Hollstein, Monika, Honold, Cornelius, Horstmann, Solveig, Hummel, Manuela, Knyazev, Yuri, Mansmann, Ulrich, Pfleger, Karin, Wagner, Simone, Weninger, Annette, Wiest, Tina   +12 more
core   +1 more source

FOXP3 Polymorphism and Upregulation of the CXCL12‐CXCR4‐SNAIL Axis with High Infiltration of M2TAM by STAT3/NFKB Pathways Influence the Survival of Cervical Cancer Patients

Advanced Biology, EarlyView.
This study highlights how cervical carcinoma progression is shaped by immune–tumor interactions within the TME. M2‐TAM infiltration, regulated through STAT3/NF‐κB and linked to CXCL12–CXCR4, promotes immune suppression, angiogenesis, and poor survival.
George A. Lira, Fábio M. de Azevedo, Ingrid G. S. Lins, Janaína C. O. Crispim, Giovanna A. Lira, Rômulo S. Cavalcante, Ricardo Cobucci, Carolina O. Mendes‐Aguiar, Rafaela Torres Dantas Da Silva, Vinícius E. da Silva, Ryan C. Q. Aquino, Raimundo F. Araújo Júnior   +11 more
wiley   +1 more source

Effective DNA/RNA co-extraction for analysis of microRNAs, mRNAs, and genomic DNA from formalin-fixed paraffin-embedded specimens. [PDF]

PLoS ONE, 2012
BACKGROUND: Retrospective studies of archived human specimens, with known clinical follow-up, are used to identify predictive and prognostic molecular markers of disease.
Adam Kotorashvili, Andrew Ramnauth, Christina Liu, Juan Lin, Kenny Ye, Ryung Kim, Rachel Hazan, Thomas Rohan, Susan Fineberg, Olivier Loudig   +9 more
doaj   +1 more source

Identification of erythroferrone as an erythroid regulator of iron metabolism. [PDF]

, 2014
Recovery from blood loss requires a greatly enhanced supply of iron to support expanded erythropoiesis. After hemorrhage, suppression of the iron-regulatory hormone hepcidin allows increased iron absorption and mobilization from stores.
Ganz, Tomas, Jung, Grace, Kautz, Léon, Nemeth, Elizabeta, Rivella, Stefano, Valore, Erika V   +5 more
core   +1 more source

Differentiation Treatment Applied to Lung Cancer Model Reduces Pathogenic Traits in Vitro

Advanced Biology, EarlyView.
Non‐small cell lung cancer cells (A549) treated with a differentiation medium showed strong reduction in proliferation, migration, clonogenicity, spheroid formation and stemness markers, with enhanced adhesion properties and alveolar features, all pointing to a notably less aggressive phenotype in support of differentiation therapy as a promising ...
Alice Grossi, Paola Fulghieri, Abdurakhmon Aduvaliev, Karen Soffiantini, Irene Oldrati, Margherita Cavallo, Marco Biggiogera, Giorgia Pellavio, Umberto Laforenza, Monica Savio, Virginie Sottile   +10 more
wiley   +1 more source

Detection of SARS-CoV-2 in fecal samples with different pretreatment methods and PCR kits

BMC Microbiology, 2021
Background Gastrointestinal symptoms are common in COVID-19 patients and SARS-CoV-2 RNA has been detected in the patients’ feces, which could lead to fecal–oral transmission.
Ranran Cao, Lirong Bao, Ming Pan, Cheng Zhang, Hongyu Liao, Li Liu, Yan Li, Mingyuan Li   +7 more
doaj   +1 more source