Results 111 to 120 of about 452,059 (305)

Immunocapture RT-qPCR Method for DWV-A Surveillance: Eliminating Hazardous Extraction for Screening Applications

open access: yesApplied Biosciences
Deformed wing virus (DWV) is a major contributor to honey bee colony losses, making effective monitoring essential for apiary management. Traditional DWV detection relies on hazardous RNA extraction followed by RT-qPCR, creating barriers for widespread ...
Krisztina Christmon   +3 more
doaj   +1 more source

Anionic Citrate‐Based 3D‐Printed Scaffolds for Tunable and Sustained Orthobiologic Delivery to Enhance Tissue Regeneration

open access: yesAdvanced Functional Materials, EarlyView.
A potent anionic citric acid‐based 3D‐printed scaffold is developed for the sustained and controlled release of orthobiologics to enhance orthopedic therapeutic efficacy. Comprehensive in vivo studies demonstrated effective bone fusion and high safety at a low dose of BMP‐2 delivered by the system, establishing it as a promising platform for safe ...
Se‐Hwan Lee   +12 more
wiley   +1 more source

RECENT SYNTHETIC ADVANCES IN 1,2,4 TRIZOLES

open access: yes, 2019
The present review is a sincere attempt to compile the reported potent triazole derivatives with significant anticonvulsant action. The triazoles are said to be the isosters of imidazoles in which the carbon atom of imidazole is isosterically replaced by nitrogen. Triazole & its derivatives have a wide range of application.
openaire   +1 more source

Dual‐Nanoparticle Engineered Hydrogel Reverses Bicellular Oxidative Stress to Accelerate Diabetic Fracture Healing

open access: yesAdvanced Functional Materials, EarlyView.
CD105‐LNPs and PS‐LNPs can deliver α‐lipoic acid to bone marrow mesenchymal stem cells and bone marrow‐derived macrophages, reversing high glucose‐induced oxidative stress while enhancing osteogenesis and M2 polarization. Integrating both nanoparticles into glucose‐responsive hyaluronic acid hydrogel forms DLNPs@HA hydrogel, which accelerates diabetic ...
Yanzhi Zhao   +11 more
wiley   +1 more source

Comparación de métodos de extracción de RNA para la detección por RT-PCR del Potato yellow vein virus (PYVV) en diferentes órganos de Solanum tuberosum Grupo Phureja

open access: yesRevista Colombiana de Biotecnología, 2013
Título en ingles: Comparison of RNA isolation methods for Potato yellow vein virus (PYVV) detection by RT-PCR in different organs of Solanum tuberosum Group Phureja Título corto: comparación de métodos de extracción de RNA Resumen Potato yellow vein ...
Anngie Katherine Hernández Guzmán   +1 more
doaj   +2 more sources

Microbubble‐Controlled Delivery of Biofilm‐Targeting Nanoparticles to Treat MRSA Infection

open access: yesAdvanced Functional Materials, EarlyView.
Here, an effective strategy using microbubble (MB)‐controlled delivery of biofilm‐targeting nanoparticles (BTNs) for removal and therapy of methicillin‐resistant Staphylococcus aureus (MRSA) biofilm infections is introduced. In vivo delivery of MB with BTN is demonstrated to silence key bacterial genes involved in biofilm formation (icaA), bacterial ...
Ju Yeon Chung   +6 more
wiley   +1 more source

The zinc finger transcription factor PLAGL2 enhances stem cell fate and activates expression of ASCL2 in intestinal epithelial cells [PDF]

open access: yes, 2010
O encaminhamento de textos originais para submissão seguem algumas orientações básicas, levando em conta, inicialmente, que devem ser inéditos e que serão submetidos à aprovação de avaliadores especialistas nos temas tratados.
Strubberg, Ashlee M   +7 more
core   +1 more source

The Tumor Cytosol miRNAs, Fluid miRNAs, and Exosome miRNAs in Lung Cancer. [PDF]

open access: yes, 2014
The focus of this review is to provide an update on the progress of microRNAs (miRNAs) as potential biomarkers for lung cancer. miRNAs are single-stranded, small non-coding RNAs that regulate gene expression and show tissue-specific signatures ...
Haisheng eXu   +4 more
core   +2 more sources

Synergistic Osteogenesis After Co‐Administration of cmRNAs Encoding BMP‐2 and BMP‐7 Utilizing a Transcript‐Activated Matrix

open access: yesAdvanced Functional Materials, EarlyView.
This study demonstrates that the dual delivery of BMP‐2/‐7 coding cmRNAs for bone healing is demonstrated as feasible, safe, and highly osteogenic. Compared to single BMP‐2 or BMP‐7 cmRNAs, the combination enhances the production of both mineral and organic components of the extracellular matrix when delivered using a collagen‐HA scaffold, supporting ...
Claudia Del Toro Runzer   +7 more
wiley   +1 more source

改良的 Trizol 法提取小鼠皮肤 RNA 技术

open access: yesZhongshan Daxue xuebao. Yixue kexue ban, 2020
【目的】由于皮肤组织韧性强且存在大量RNA酶,采用经典Trizol法提取的RNA存在易降解质量低等不足。因此,本研究旨在通过改良的Trizol法实现高质量的皮肤RNA提取。【方法】采用不同处理方式(Tri:Trizol包埋;Pro:RNA样本保护液包埋;Cry:先冷冻液氮再冷冻-80℃冰箱;LNG:液氮研磨;Cut:采用剪刀剪碎组织)提取的小鼠正常皮肤RNA为实验组;脊髓组织作为参照组,并以咪喹莫特(IMQ)诱导的小鼠银屑病模型皮肤组织作为保真性验证组。我们采用紫外分光光度法、琼脂凝胶电泳法 ...
林震嘉   +7 more
doaj  

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