Results 111 to 120 of about 463,109 (314)
New generation of electrochemical immunoassay based on polymeric nanoparticles for early detection of breast cancer [PDF]
Screening and early diagnosis are the key factors for the reduction of mortality rate and treatment cost of cancer. Therefore, sensitive and selective methods that can reveal the low abundance of cancer biomarkers in a biological sample are always ...
Al-Hetlani, Entesar +5 more
core +1 more source
Sanger Tree of Life RNA Extraction: Manual TRIzol™ v1
This protocol describes the manual extraction of RNA from multiple different tissue samples intended for RNA-Seq, using the TRIzol™ Reagent, based on the Thermo Fisher TRIzol™ Reagent User Guide. This process is highly effective for most taxonomic groups covered by the Tree of Life Programme.
Raquel Juliana Vionette do Amaral +2 more
openaire +1 more source
An oral nanoplatform, MOP@T@D, which can maintain glucose homeostasis and restore islet β cells in diabetic rats is developed. It achieves efficient intestinal absorption and liver‐targeted delivery. The nanoparticle disintegrates only in response to hyperglycemia to release insulin on demand and provides antioxidant protection through selenoprotein ...
Chenxiao Chu +14 more
wiley +1 more source
A mechanically tunable hydrogel composed of gelatin, chondroitin sulfate and laminin promotes angiogenesis in vitro without the supplement of growth factors. Endothelial cells morphogenesis was further enhanced by medium conditioned with bioactive glass 58S‐released ions (Ca and Si), thus offering a promising strategy to vascularize 3D tissue ...
Marco Piazzoni +13 more
wiley +1 more source
Título en ingles: Comparison of RNA isolation methods for Potato yellow vein virus (PYVV) detection by RT-PCR in different organs of Solanum tuberosum Group Phureja Título corto: comparación de métodos de extracción de RNA Resumen Potato yellow vein ...
Anngie Katherine Hernández Guzmán +1 more
doaj +2 more sources
Synthetic Nanobiology Actuated Lipometabolic Cell Factory for Autologous Tumor Immunotherapy
FA plays a crucial role in the interaction between tumor cells and the tumor microenvironment, especially for the immune response. A biocatalytic immunoenhancement strategy is developed to boost antitumor immunity by FA metabolic orientation to ceramide. Through the design of this delicate catalytic immunoenhancement strategy, the synthetic nanobiology
Shoujie Zhao +8 more
wiley +1 more source
A self‐gelling PG@PAC (POD/Gel‐CDH@PA/CHX) powder is developed for infected burn care in austere settings. Upon contact with wound exudate, it instantly forms an adhesive hydrogel, providing simultaneous hemostasis, broad‐spectrum antibacterial activity, reactive oxygen species scavenging, and immunomodulation. In a murine model of S.
Liping Zhang +14 more
wiley +1 more source
【目的】由于皮肤组织韧性强且存在大量RNA酶,采用经典Trizol法提取的RNA存在易降解质量低等不足。因此,本研究旨在通过改良的Trizol法实现高质量的皮肤RNA提取。【方法】采用不同处理方式(Tri:Trizol包埋;Pro:RNA样本保护液包埋;Cry:先冷冻液氮再冷冻-80℃冰箱;LNG:液氮研磨;Cut:采用剪刀剪碎组织)提取的小鼠正常皮肤RNA为实验组;脊髓组织作为参照组,并以咪喹莫特(IMQ)诱导的小鼠银屑病模型皮肤组织作为保真性验证组。我们采用紫外分光光度法、琼脂凝胶电泳法 ...
林震嘉 +7 more
doaj
Genetic analysis of members of the species Oropouche virus and identification of a novel M segment sequence [PDF]
Oropouche virus (OROV) is a public health threat in South America, and in particular Northern Brazil, causing frequent outbreaks of febrile illness.
Azevedo, Raimunda S.S. +8 more
core +1 more source
A UV‐triggered injectable dual‐network hydrogel is reported as the first application of bletilla striata polysaccharide (BSP) in osteochondral repair. By integrating methacrylamide‐modified BSP and nitrobenzaldehyde‐functionalized hyaluronic acid, the system achieves immunomodulation, mechanical reinforcement, and dynamic tissue adhesion, thereby ...
Jiaming Cui +10 more
wiley +1 more source

