Results 131 to 140 of about 452,059 (305)
RNA extraction using the 'home-made' Trizol substitute v1
, 2016 Asimple protocol for RNA extraction from various types of samples (protists, fungi, bacteria, organelles, subcellular fractions, ribosomes, etc.). Compared tothe commercial Trizol® procedure, it makes use ofgenerally available chemicals without the need for columns,thusallowing concurrent extraction oftranscripts of all sizes (including RNAs <200 nt)
openaire +2 more sources
Cell Surface Thiol Engineering Mechanoregulates Myogenic Differentiation via the FAK–PI3K–AKT Axis
Advanced Healthcare Materials, EarlyView.Schematic diagram illustrating how cell surface modification of skeletal muscle progenitor cells through TCEP treatment reveals enhanced cell adhesion, intracellular tension, and myogenesis at 19.66 kPa stiffness, leading to optimal cell fusion. In contrast, no significant changes are observed in the softer (10.61 kPa) or stiffer (49.4 kPa) matrices ...
Juyeon Kim +10 more
wiley +1 more source
Biologia molecular aplicada às dermatoses tropicais Molecular biology in tropical dermatoses
Anais Brasileiros de Dermatologia, 2008 São apresentados conceitos básicos sobre célula, código genético e síntese protéica, e sobre algumas técnicas de biologia molecular, tais como PCR, PCR-RFLP, seqüenciamento de DNA, RT-PCR e immunoblotting.
Ana Maria Roselino
doaj +1 more source
Identification of the alternative oxidase gene and its expression in the copepod Tigriopus californicus [PDF]
, 2019 In addition to the typical electron transport system (ETS) in animal mitochondria responsible for oxidative phosphorylation, in some species there exists an alternative oxidase (AOX) pathway capable of catalyzing the oxidation of ubiquinol and the ...
Cygelfarb, Willie +3 more
core +1 more source
RNA Isolation from Plant Tissue Protocol 10: TRIzol LS Reagent Method v1 [PDF]
, 2019 Eric Carpenter
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Advanced Healthcare Materials, EarlyView.A tunable methacrylated decellularized bone matrix hydrogel (dECM‐MA) is developed to support 3D culture of human osteoblasts. The hydrogel preserves bone‐specific ECM cues and allows precise control over mechanical properties. This system provides a customizable platform for studying osteogenic differentiation and modeling bone tissue environments for
Minne Dekker +5 more
wiley +1 more source
ParasitologiaBalamuthia mandrillaris is a free-living amoeba (FLA) that causes granulomatous amebic encephalitis (GAE) and skin lesions. Transcriptomic analysis is a powerful tool used to study B. mandrillaris pathogenic infections.
Leobardo Daniel Gonzalez-Zuñiga +5 more
doaj +1 more source
PLoS ONE, 2018 Human Multipotent Stromal Cells (MSCs) are a valuable resource for regenerative medicine and are widely studied. They can be isolated from a variety of tissues and differentiate into multiple cell types (multi-potent).
Bas Brinkhof +5 more
doaj +1 more source
Micro-RNA mediated regulation of a cytokine factor: TNF-alpha: an exploration of gene expression control in proliferating and quiescent cells [PDF]
, 2016 Two types mechanisms that control gene expression involve cis-regulatory factors and trans-regulatory factors. Cis-acting regulatory RNAs include targeted messenger RNA (mRNA) specificity and AU-rich elements (AREs).
Bhambhani, Vijeta
core +1 more source
RNA Extraction from Drosophila Tissues using TRIzol Reagent v1 [PDF]
, 2016 This protocol is adapted from the Invitrogen Life Technologies Trizol manual.
openaire +1 more source