Results 151 to 160 of about 461,258 (325)

Evaluation and Standardization of RNA Extractions with Quality for RNA-Seq for Balamuthia mandrillaris

Parasitologia
Balamuthia mandrillaris is a free-living amoeba (FLA) that causes granulomatous amebic encephalitis (GAE) and skin lesions. Transcriptomic analysis is a powerful tool used to study B. mandrillaris pathogenic infections.
Leobardo Daniel Gonzalez-Zuñiga, Libia Zulema Rodriguez-Anaya, Jose Reyes Gonzalez-Galaviz, Abraham Cruz-Mendívil, Fernando Lares-Villa, Luis Fernando Lares-Jiménez   +5 more
doaj   +1 more source

Improving characterisation of human Multipotent Stromal Cells cultured in 2D and 3D: Design and evaluation of primer sets for accurate gene expression normalisation.

PLoS ONE, 2018
Human Multipotent Stromal Cells (MSCs) are a valuable resource for regenerative medicine and are widely studied. They can be isolated from a variety of tissues and differentiate into multiple cell types (multi-potent).
Bas Brinkhof, Huidong Jia, Bo Zhang, Zhanfeng Cui, Hua Ye, Hui Wang   +5 more
doaj   +1 more source

Silencing Myostatin Using In Vivo Self‐Assembled siRNA Protects Against Cancer‐ and Dexamethasone‐Induced Muscle Atrophy

Advanced Healthcare Materials, EarlyView.
This study reports an in vivo self‐assembled siRNA strategy that enables the liver to generate small extracellular vesicles (sEVs) tagged with a muscle‐targeting peptide (MSP) and naturally loaded with myostatin (MSTN)‐siRNA. These MSP‐tagged sEVs are systemically delivered to skeletal muscle, efficiently silence MSTN, promote muscle hypertrophy, and ...
Xin Yin, Azhar Anwar, Jiehao Chen, Qinghao Sun, Likun Zhou, Jie Tang, Jingwei Guo, Linbo Yan, Yongci Chen, Feng Yin, Chen‐Yu Zhang, Zigang Li, Jizheng Ma, Liyuan Sheng, Xi Chen   +14 more
wiley   +1 more source

Few‐Layered Conductive Graphene Foams for Electrical Transdifferentiation of Mesenchymal Stem Cells Into Schwann Cell‐Like Phenotypes

Advanced Healthcare Materials, EarlyView.
Few‐layered three dimansional conductive graphene foams are promising cytocompatible platforms to transdifferentiate mesenchymal stem cells into Schwann cell‐like phenotypes using electrical and microstructural cues. Applied electrical stimulation conditions resulted in activation of MAPK, neurotrphin and RAS signaling pathways that led to upregulation
Ekin G. Simsar, Peifu Cheng, Tugce Dogruel, Maxsam Donta, Juhyung Jung, Naomi A. Asante, Donald S. Sakaguchi, Surya K. Mallapragada, Piran R. Kidambi, Metin Uz   +9 more
wiley   +1 more source

The zinc finger transcription factor PLAGL2 enhances stem cell fate and activates expression of ASCL2 in intestinal epithelial cells [PDF]

, 2010
O encaminhamento de textos originais para submissão seguem algumas orientações básicas, levando em conta, inicialmente, que devem ser inéditos e que serão submetidos à aprovação de avaliadores especialistas nos temas tratados.
Strubberg, Ashlee M, Veronese Paniagua, Daniel A, Zhao, Tingting, Dublin, Leeran, Pritchard, Thomas, Bayguinov, Peter O, Fitzpatrick, James A.J., Madison, Blair B   +7 more
core   +1 more source

Multiscale Hybrid Surface Topographies Orchestrate Immune Regulation, Antibacterial Defense, and Tissue Regeneration

Advanced Healthcare Materials, EarlyView.
Hybrid wrinkled topographies coordinate immune, tissue, and bacterial interactions. The surfaces promote osteointegration, tune macrophage polarization, and inhibit biofilm formation, highlighting a multifunctional strategy for next‐generation implant design.
Mohammad Asadi Tokmedash, Jacob Robins, J. Scott VanEpps, Minji Kim, Jouha Min   +4 more
wiley   +1 more source

Preparation of Bifunctional Orthosilicophosphate MgO‐CaO‐ZnO‐P2O5‐SiO2 Glasses: in vitro Evaluation of Antibacterial Activity and Osteoblast Gene Expression Behavior

Advanced Healthcare Materials, EarlyView.
Bifunctional ZnO‐containing orthosilicophosphate glasses (SPGs) composed mainly of orthotetrahedral groups with the unique glass network structure without long‐chain units are prepared via melt‐quenching. The glasses exhibit excellent antibacterial activity and upregulate the expression of osteogenic markers by releasing inorganic ions.
Sungho Lee, Hayato Asano, Makoto Sakurai, Takayoshi Nakano, Toshihiro Kasuga   +4 more
wiley   +1 more source

RNA Isolation from Plant Tissue Protocol 10: TRIzol LS Reagent Method v1 [PDF]

, 2019
Eric Carpenter
openalex   +1 more source

Borate‐Ion‐Stimulated Macrophages Promote Osteogenic Differentiation of Mesenchymal Stem Cells

Advanced Healthcare Materials, EarlyView.
The immune responses of mouse macrophages stimulated by borate ions promote the osteogenic differentiation and mineralization of mouse bone marrow–derived mesenchymal stem cells in an ion‐dose‐dependent manner by providing cytokines, including BMP‐2.
Kazumasa Ikedo, Hiroki Hatakeyama, Sayaka Oguri, Akiko Obata, Toshihiro Kasuga   +4 more
wiley   +1 more source

Ascorbic Acid Modulates Collagen Properties in Glucocorticoid‐Induced Osteoporotic Bone: Insights into Chemical, Mechanical, and Biological Regulation

Advanced Healthcare Materials, EarlyView.
Osteoporosis from long‐term glucocorticoid (GIOP) use elevates susceptibility to fracture. This study shows GCs impair ascorbic acid (AA) metabolism in osteoblasts, collagen synthesis and extracellular matrix integrity. AA enhanced collagen biochemical and mechanical properties and restored osteoblast and endothelial function. These findings underscore
Micaila DE Curtis, Yoontae Kim, Rahul Patil, Xinran Zhang, Meth Jayatilake, Orelle Bulgin, Peter Lialios, Sana Surrency, Soraya Tarrah, Argyris K. Lazaris, Alison Grafton, James Williams IV, Seyedeh Boshra Nouraie, Maria Bizaki, Penelope Xeron, Edward Van Keuren, Jonathan E. Seppala, Evagelia C. Laiakis, Joanna B. Kitlinska, Stella Alimperti   +19 more
wiley   +1 more source