Results 291 to 300 of about 447,474 (316)

Effectiveness of TRIzol in Inactivating Animal Pathogens.

Applied Biosafety, 2023
Introduction Safe handling of biological samples sourced from wild ecosystems is a pressing concern for scientists in disparate fields, including ecology and evolution, OneHealth initiatives, bioresources, geography, veterinary medicine, conservation ...
Gwen Duytschaever, P. R. Ströher, Kevin Fonseca, F. van der Meer, A. Melin   +4 more
semanticscholar   +3 more sources

Purification of RNA using TRIzol (TRI reagent).

Cold Spring Harbor Protocols, 2010
TRIzol solubilization and extraction is a relatively recently developed general method for deproteinizing RNA. This method is particularly advantageous in situations where cells or tissues are enriched for endogenous RNases or when separation of ...
D. Rio, M. Ares, G. Hannon, T. Nilsen
semanticscholar   +4 more sources

Proteome analysis of heart biopsies using a TRIzol-based protein extraction.

Clinica Chimica Acta, 2015
When dealing with rare samples of which only minute amounts are available, e.g. human heart tissue, simultaneous extraction of DNA, RNA, and proteins from the same sample is crucial for a comprehensive analysis on the physiological or pathological state of such precious tissue. In this study we provethe efficacy of a modified TRIzol protocol to extract
C. Pop, S. Ameling, K. Empen, Julia Rüdebusch, V. Dhople, S. Felix, F. Loghin, U. Völker, E. Hammer   +8 more
semanticscholar   +3 more sources

Oral Squamous Cell Carcinoma Lipid Evaluation in Gingiva Tissue Stored in TRIzol via Shotgun Lipidomics and MALDI Mass Spectrometry Imaging.

Journal of Proteome Research, 2023
Oral squamous cell carcinoma (OSCC) is the prevalent type of oral cavity cancer, requiring precise, accurate, and affordable diagnosis to identify the disease in early stages, Comprehending the differences in lipid profiles between healthy and cancerous ...
R. A. Bernardo, C. I. de Oliveira Júnior, J. V. Roque, N. L. Costa, V. M. Roriz, C. Sorgi, C. Janfelt, B. Vaz, A. Chaves   +8 more
semanticscholar   +1 more source

An improved Trizol method for extracting total RNA from Eleutherococcus senticosus (Rupr. & Maxim.) Maxim leaves.

Phytochemical Analysis
INTRODUCTION High-quality nucleic acids are the basis for molecular biology experiments. Traditional RNA extraction methods are not suitable for Eleutherococcus senticosus Maxim.
Siqiu Xiao, Ying Zhang, Xiaoqing Tang, Jing Yang, Weixue Zhong, Ye Zhang, Ying Liu, Dewen Li   +7 more
semanticscholar   +1 more source

Detection of trizole contaminated waste water using biocatalyst and effective biodegradation potential of flubendiamide

Environmental Research, 2022
Flubendiamide is a new class of chemical pesticide with broad spectrum activity against lepidopteran pests. Due to limited approach and high specificity towards various non targeted organisms, the unrestricted application of this pesticide as a prominent alternate for organochlorine and organophosphate pesticides, causing serious environmental ...
Arshad Mehmood Abbasi, M. Ajmal Ali, Amal M. Al-Mohaimeed, J.R. Abima Shazhni   +3 more
openaire   +2 more sources

Effects of RBC removal and TRIzol of peripheral blood samples on RNA stability

Clinica Chimica Acta, 2011
Purification of mRNA from stored specimens is very important because results from RT-PCR and microarray analyses are largely affected by the quality of mRNA. Moreover, many preanalytical factors during collection, processing, and storage may affect mRNA quality and the expression of peripheral blood mononuclear cells (PBMC).
Jin Hyeon Lee, Do Youn Park, Sang-Hyun Hwang, Jeong-Eun Kang, Hyeong-Hoi Kim   +4 more
openaire   +3 more sources