Results 291 to 300 of about 452,059 (305)

Potential prognostic biomarker MIER2 in colon adenocarcinoma: from data mining to validation. [PDF]

Clin Exp Med
Zhao C, Li X, Zhu S, Shi L.
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Proteins are a source of glycans found in preparations of glycoRNA

Kegel NB, Demirel NY, Glatter T, Höfer K, Kaufmann A, Bauer S.   +5 more
europepmc   +1 more source

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Effectiveness of TRIzol in Inactivating Animal Pathogens

Applied Biosafety, 2023
Safe handling of biological samples sourced from wild ecosystems is a pressing concern for scientists in disparate fields, including ecology and evolution, OneHealth initiatives, bioresources, geography, veterinary medicine, conservation, and many others. This is especially relevant given the growing global research community and collaborative networks
Gwen Duytschaever, Patrícia R. Ströher, Kevin Fonseca, Frank van der Meer, Amanda D. Melin   +4 more
openaire   +2 more sources

Purification of RNA Using TRIzol (TRI Reagent)

Cold Spring Harbor Protocols, 2010
INTRODUCTIONTRIzol solubilization and extraction is a relatively recently developed general method for deproteinizing RNA. This method is particularly advantageous in situations where cells or tissues are enriched for endogenous RNases or when separation of cytoplasmic RNA from nuclear RNA is impractical.
Timothy W. Nilsen, Manuel Ares, Donald C. Rio, Gregory J. Hannon   +3 more
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Detection of trizole contaminated waste water using biocatalyst and effective biodegradation potential of flubendiamide

Environmental Research, 2022
Flubendiamide is a new class of chemical pesticide with broad spectrum activity against lepidopteran pests. Due to limited approach and high specificity towards various non targeted organisms, the unrestricted application of this pesticide as a prominent alternate for organochlorine and organophosphate pesticides, causing serious environmental ...
Arshad Mehmood Abbasi, M. Ajmal Ali, Amal M. Al-Mohaimeed, J.R. Abima Shazhni   +3 more
openaire   +2 more sources

Effects of RBC removal and TRIzol of peripheral blood samples on RNA stability

Clinica Chimica Acta, 2011
Purification of mRNA from stored specimens is very important because results from RT-PCR and microarray analyses are largely affected by the quality of mRNA. Moreover, many preanalytical factors during collection, processing, and storage may affect mRNA quality and the expression of peripheral blood mononuclear cells (PBMC).
Jin Hyeon Lee, Do Youn Park, Sang-Hyun Hwang, Jeong-Eun Kang, Hyeong-Hoi Kim   +4 more
openaire   +3 more sources

Trizol-based method for sample preparation and isoelectric focusing of halophilic proteins

Analytical Biochemistry, 2006
A persisting complication in the development of well-resolved two-dimensional PAGE maps of halophilic proteins is their natural incompatibility with isoelectric focusing (IEF). The complete desalting of samples, which is necessary for IEF, tends to aggregate halophilic proteins, often requires relatively large amounts of starting material due to ...
Jennifer Busby, Stan Stevens, Julie A. Maupin-Furlow, Phillip Aaron Kirkland   +3 more
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Proteome analysis of heart biopsies using a TRIzol-based protein extraction

Clinica Chimica Acta, 2015
When dealing with rare samples of which only minute amounts are available, e.g. human heart tissue, simultaneous extraction of DNA, RNA, and proteins from the same sample is crucial for a comprehensive analysis on the physiological or pathological state of such precious tissue. In this study we provethe efficacy of a modified TRIzol protocol to extract
Cristina Pop, Sabine Ameling, Klaus Empen, Julia Rüdebusch, Vishnu Mukund Dhople, Stephan B. Felix, Felicia Loghin, Uwe Völker, Elke Hammer   +8 more
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Proteins isolated with TRIzol are compatible with two-dimensional electrophoresis and mass spectrometry analyses

Analytical Biochemistry, 2012
TRIzol is used for RNA isolation but also permits protein recovery. We investigated whether proteins prepared with TRIzol were suitable for two-dimensional gel electrophoresis (2-DE) and matrix-assisted laser desorption/ionization mass spectrometry.
Young C, Truman P
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