Results 301 to 310 of about 447,474 (316)
Some of the next articles are maybe not open access.
Analytical Biochemistry, 2012
TRIzol is used for RNA isolation but also permits protein recovery. We investigated whether proteins prepared with TRIzol were suitable for two-dimensional gel electrophoresis (2-DE) and matrix-assisted laser desorption/ionization mass spectrometry.
Young C, Truman P
openaire +4 more sources
TRIzol is used for RNA isolation but also permits protein recovery. We investigated whether proteins prepared with TRIzol were suitable for two-dimensional gel electrophoresis (2-DE) and matrix-assisted laser desorption/ionization mass spectrometry.
Young C, Truman P
openaire +4 more sources
Trizol-based method for sample preparation and isoelectric focusing of halophilic proteins
Analytical Biochemistry, 2006A persisting complication in the development of well-resolved two-dimensional PAGE maps of halophilic proteins is their natural incompatibility with isoelectric focusing (IEF). The complete desalting of samples, which is necessary for IEF, tends to aggregate halophilic proteins, often requires relatively large amounts of starting material due to ...
Jennifer Busby +3 more
openaire +3 more sources
RNA Extraction from Drosophila Tissues using TRIzol Reagent
, 2016MATERIALS N AM E CATAL OG # VEN DOR Isopropanol Chloroform TRIzol Reagent 15596026 Bio Plas Disposable Homogenization Pestles BPI-4040-PB Microcentrifuge Tubes Temperature-regulated centrifuge Ultrapure Distilled, Nuclease Free Water Filter Tips Dry Ice ...
E. Allen
semanticscholar +1 more source
Clinical Laboratory, 2015
BACKGROUND MicroRNAs (miRNAs) are a group of small endogenous, non-coding RNA molecules that have been demonstrated to be essential regulators of many critical biological and pathological processes. Because of their high stability in blood and the strong
Weifeng Lv +8 more
semanticscholar +1 more source
BACKGROUND MicroRNAs (miRNAs) are a group of small endogenous, non-coding RNA molecules that have been demonstrated to be essential regulators of many critical biological and pathological processes. Because of their high stability in blood and the strong
Weifeng Lv +8 more
semanticscholar +1 more source
Talanta: The International Journal of Pure and Applied Analytical Chemistry, 2023
Cheng-Yen Kao +5 more
semanticscholar +1 more source
Cheng-Yen Kao +5 more
semanticscholar +1 more source
, 2015
Background: The study of RNA, DNA, and protein from the same sample is a great advantage but can be challenging. Using Trizol, one can simultaneously extract RNA, DNA, and protein, leading to efficient sample use and more comprehensive analysis. Although
C. Pop +5 more
semanticscholar +1 more source
Background: The study of RNA, DNA, and protein from the same sample is a great advantage but can be challenging. Using Trizol, one can simultaneously extract RNA, DNA, and protein, leading to efficient sample use and more comprehensive analysis. Although
C. Pop +5 more
semanticscholar +1 more source
A simple and loss-free method to remove TRIzol contaminations from minute RNA samples
Analytical Biochemistry, 2009Acid guanidium phenol preparations such as TRIzol allow the reproducible isolation of high-quality total RNA from various sources. However, if applied to minimal sample sizes, the quality parameters of the isolated RNA are often low. Here we present an approach to improve the 260/280- and 230/260-nm ratios of such preparations as well as the ratio of ...
Marlis Fischaleck +2 more
openaire +3 more sources
, 2014
Objective To set up a safe,easy and reliable method of viral RNA extraction from serum samples.Methods Three viral RNA extraction methods were compared by using real-time RT-PCR,using QIAamp Viral RNA Mini Kit,TRIzol(or TriPure) reagents adapted to ...
Wei-Hong Li +6 more
semanticscholar +1 more source
Objective To set up a safe,easy and reliable method of viral RNA extraction from serum samples.Methods Three viral RNA extraction methods were compared by using real-time RT-PCR,using QIAamp Viral RNA Mini Kit,TRIzol(or TriPure) reagents adapted to ...
Wei-Hong Li +6 more
semanticscholar +1 more source