Results 301 to 310 of about 461,258 (325)

Purification of RNA Using TRIzol (TRI Reagent)

Cold Spring Harbor Protocols, 2010
INTRODUCTIONTRIzol solubilization and extraction is a relatively recently developed general method for deproteinizing RNA. This method is particularly advantageous in situations where cells or tissues are enriched for endogenous RNases or when separation of cytoplasmic RNA from nuclear RNA is impractical.
Donald C, Rio, Manuel, Ares, Gregory J, Hannon, Timothy W, Nilsen   +3 more
openaire   +3 more sources

Proteome analysis of heart biopsies using a TRIzol-based protein extraction

Clinica Chimica Acta, 2015
When dealing with rare samples of which only minute amounts are available, e.g. human heart tissue, simultaneous extraction of DNA, RNA, and proteins from the same sample is crucial for a comprehensive analysis on the physiological or pathological state of such precious tissue. In this study we provethe efficacy of a modified TRIzol protocol to extract
Cristina Pop, Sabine Ameling, Klaus Empen, Julia Rüdebusch, Vishnu Mukund Dhople, Stephan B. Felix, Felicia Loghin, Uwe Völker, Elke Hammer   +8 more
openaire   +3 more sources

Oral Squamous Cell Carcinoma Lipid Evaluation in Gingiva Tissue Stored in TRIzol via Shotgun Lipidomics and MALDI Mass Spectrometry Imaging.

Journal of Proteome Research, 2023
Oral squamous cell carcinoma (OSCC) is the prevalent type of oral cavity cancer, requiring precise, accurate, and affordable diagnosis to identify the disease in early stages, Comprehending the differences in lipid profiles between healthy and cancerous ...
R. A. Bernardo, C. I. de Oliveira Júnior, J. V. Roque, N. L. Costa, V. M. Roriz, C. Sorgi, C. Janfelt, B. Vaz, A. Chaves   +8 more
semanticscholar   +1 more source

An improved Trizol method for extracting total RNA from Eleutherococcus senticosus (Rupr. & Maxim.) Maxim leaves.

Phytochemical Analysis
INTRODUCTION High-quality nucleic acids are the basis for molecular biology experiments. Traditional RNA extraction methods are not suitable for Eleutherococcus senticosus Maxim.
Siqiu Xiao, Ying Zhang, Xiaoqing Tang, Jing Yang, Weixue Zhong, Ye Zhang, Ying Liu, Dewen Li   +7 more
semanticscholar   +1 more source

Trizol-based method for sample preparation and isoelectric focusing of halophilic proteins

Analytical Biochemistry, 2006
A persisting complication in the development of well-resolved two-dimensional PAGE maps of halophilic proteins is their natural incompatibility with isoelectric focusing (IEF). The complete desalting of samples, which is necessary for IEF, tends to aggregate halophilic proteins, often requires relatively large amounts of starting material due to ...
P Aaron, Kirkland, Jennifer, Busby, Stan, Stevens, Julie A, Maupin-Furlow   +3 more
openaire   +2 more sources

Effects of RBC removal and TRIzol of peripheral blood samples on RNA stability

Clinica Chimica Acta, 2011
Purification of mRNA from stored specimens is very important because results from RT-PCR and microarray analyses are largely affected by the quality of mRNA. Moreover, many preanalytical factors during collection, processing, and storage may affect mRNA quality and the expression of peripheral blood mononuclear cells (PBMC).
Jeong-Eun, Kang, Sang-Hyun, Hwang, Jin Hyeon, Lee, Do Youn, Park, Hyeong-Hoi, Kim   +4 more
openaire   +2 more sources

Simplified protocol modification of TRIzol method for Extraction of high-quality RNA yield from RNase-rich rat pancreas

Process Biochemistry, 2023
Maha Osman Hammad
semanticscholar   +1 more source