Results 31 to 40 of about 447,474 (316)
RNA extraction using the 'home-made' Trizol substitute
Asimple protocol for RNA extraction from various types of samples (protists, fungi, bacteria, organelles, subcellular fractions, ribosomes, etc.). Compared tothe commercial Trizol® procedure, it makes use ofgenerally available chemicals without the need for columns,thusallowing concurrent extraction oftranscripts of all sizes (including RNAs <200 nt)
M. Valach
semanticscholar +3 more sources
RNA extraction from Synechocystis sp. PCC 6803 with Trizol reagent [PDF]
Dennis Dienst
semanticscholar +2 more sources
Pigeonpea (Cajanuscajan L.) is a legume crop that contains high levels of polyphenolic compounds and polysaccharides that become a hindrance in extracting good-quality and enough amount of RNA from its tissues. With the existing methods of RNA isolation,
Pawan Mainkar+7 more
openalex +3 more sources
MicroRNA Isolation by Trizol-Based Method and Its Stability in Stored Serum and cDNA Derivatives [PDF]
MicroRNAs (miRNAs) are small, non-coding RNA molecules that regulate gene expression at the post-transcriptional level. Since aberrant expression of miRNAs has been proposed as usage for blood-based biomarkers, hence reliable techniques for miRNA ...
Keson Trakunram+4 more
openalex +2 more sources
Evaluation of the Use of TRIzol-Based Protein Extraction Approach for Gel-Based Proteomic Analysis of Dried Seafood Products and Chinese Tonic Foods [PDF]
Although the emergence of gel-free approaches has greatly enhanced proteomic studies, two-dimensional gel electrophoresis (2-DE) remains one of the most widely used proteomic techniques for its high resolving power, relatively low cost, robustness, and ...
Kin-Ka Chan+3 more
openalex +2 more sources
Synthesis and Study the Chelating Activity of some Polymers Containing Heterocyclic Rings, The Derivatives from 1,2,4- trizol Levofloxacin acid [PDF]
This is an Open Access article licensed under a Creative Commons Attribution-Non Commercial-Share Alike 4.0 International License (https://creativecommons.org/licenses/by-nc-sa/4.0/), which permits unrestricted Non Commercial use, distribution and ...
Tahseen Saddam Fandi Al-Mathkuri+2 more
openalex +2 more sources
Extraction RNA Protocol from Blood and Testis Tissue of Local Roosters
The (one-step) method has become widely used to isolate total RNA from living organism samples and from different tissues. The aim of this study is to extract RNA from the blood and testicular tissue of male local chickens.
Ahmed Khalid+3 more
doaj +1 more source
Virtual in situs: Sequencing mRNA from cryo-sliced Drosophila embryos to determine genome-wide spatial patterns of gene expression [PDF]
Complex spatial and temporal patterns of gene expression underlie embryo differentiation, yet methods do not yet exist for the efficient genome-wide determination of spatial expression patterns during development.
Combs, Peter A., Eisen, Michael B.
core +15 more sources
The high quality, yield and purity total RNA samples are essential for molecular experiments. However, harvesting high quality RNA in Lilium davidii var.
Chunlei Wang+7 more
doaj +1 more source