Results 31 to 40 of about 461,258 (325)
An Optimised TRIzol-based Protocol for the Improvement of RNA Extraction Yield of Tomato Stem [PDF]
Pertanika Journal of Tropical Agricultural Science, 2021 One of the most common methods for purifying RNA is using TRIzol reagent because of its simplicity and economic feasibility. However, the drawback of this method is frequently the low quality of extracted RNA due to contaminants from the residue of ...
Anis Afifah +5 more
openalex +2 more sources
TRIzol-based RNA Extraction: A Reliable Method for Gene Expression Studies [PDF]
Journal of Sciences, Islamic Republic of Iran, 2014 RNA extraction is a prerequisite technique for gene expression studies, analyzing the etiology and disease progression, treatment effects, as well as designing the diagnostic methods.
M. Pahlevan Kakhki
doaj +2 more sources
A TRIzol-based method for high recovery of plasma sncRNAs approximately 30 to 60 nucleotides [PDF]
Scientific Reports, 2022 Protein functional effector sncRNAs (pfeRNAs) are approximately 30–60 nucleotides (nt), of which the extraction method from plasma has not yet been reported.
Kristen Rodgers +6 more
openalex +2 more sources
MicroRNA Isolation by Trizol-Based Method and Its Stability in Stored Serum and cDNA Derivatives [PDF]
Asian Pacific Journal of Cancer Prevention, 2019 MicroRNAs (miRNAs) are small, non-coding RNA molecules that regulate gene expression at the post-transcriptional level. Since aberrant expression of miRNAs has been proposed as usage for blood-based biomarkers, hence reliable techniques for miRNA ...
Keson Trakunram +4 more
openalex +2 more sources
A TRIzol-Beadbeater RNA extraction method for yeast v1 [PDF]
Saccharomyces cerevisiae yeast cells were harvested by centrifugation and preserved in TRIzol reagent at −80 °C. Cell lysis was achieved via bead-beating in the presence of zirconium oxide beads using a high-speed homogenizer (Omni Bead Ruptor 12), resulting in >85% lysis after a single 30-second cycle, as verified by phase-contrast microscopy.
Sangeet Honey, Bruce Futcher
openalex +2 more sources
Virtual in situs: Sequencing mRNA from cryo-sliced Drosophila embryos to determine genome-wide spatial patterns of gene expression [PDF]
, 2013 Complex spatial and temporal patterns of gene expression underlie embryo differentiation, yet methods do not yet exist for the efficient genome-wide determination of spatial expression patterns during development.
Combs, Peter A., Eisen, Michael B.
core +21 more sources
Isolation of RNA from Grains of Medicinal Rice “Njavara” using Improved TRIzol Method [PDF]
, 2020 Njavara rice, indigenous to Kerala, is a unique grain plant in the Oryza genus. It is used in the traditional medicine for the treatment of various diseases related to circulatory, respiratory and digestive ailments (Reshmi and Nandini, 2013).
Y. S. Wagh +7 more
openalex +2 more sources