Results 31 to 40 of about 447,474 (316)

RNA extraction using the 'home-made' Trizol substitute

open access: yes, 2016
Asimple protocol for RNA extraction from various types of samples (protists, fungi, bacteria, organelles, subcellular fractions, ribosomes, etc.). Compared tothe commercial Trizol® procedure, it makes use ofgenerally available chemicals without the need for columns,thusallowing concurrent extraction oftranscripts of all sizes (including RNAs <200 nt)
M. Valach
semanticscholar   +3 more sources

Development of modified CTAB and Trizol protocols to isolate high molecular weight (HMW) RNA from polyphenol and polysaccharides rich pigeonpea (Cajanuscajan (L.) Millsp

open access: goldPLoS ONE, 2023
Pigeonpea (Cajanuscajan L.) is a legume crop that contains high levels of polyphenolic compounds and polysaccharides that become a hindrance in extracting good-quality and enough amount of RNA from its tissues. With the existing methods of RNA isolation,
Pawan Mainkar   +7 more
openalex   +3 more sources

MicroRNA Isolation by Trizol-Based Method and Its Stability in Stored Serum and cDNA Derivatives [PDF]

open access: goldAsian Pacific Journal of Cancer Prevention, 2019
MicroRNAs (miRNAs) are small, non-coding RNA molecules that regulate gene expression at the post-transcriptional level. Since aberrant expression of miRNAs has been proposed as usage for blood-based biomarkers, hence reliable techniques for miRNA ...
Keson Trakunram   +4 more
openalex   +2 more sources

Evaluation of the Use of TRIzol-Based Protein Extraction Approach for Gel-Based Proteomic Analysis of Dried Seafood Products and Chinese Tonic Foods [PDF]

open access: goldInternational Journal of Molecular Sciences, 2018
Although the emergence of gel-free approaches has greatly enhanced proteomic studies, two-dimensional gel electrophoresis (2-DE) remains one of the most widely used proteomic techniques for its high resolving power, relatively low cost, robustness, and ...
Kin-Ka Chan   +3 more
openalex   +2 more sources

Synthesis and Study the Chelating Activity of some Polymers Containing Heterocyclic Rings, The Derivatives from 1,2,4- trizol Levofloxacin acid [PDF]

open access: gold, 2018
This is an Open Access article licensed under a Creative Commons Attribution-Non Commercial-Share Alike 4.0 International License (https://creativecommons.org/licenses/by-nc-sa/4.0/), which permits unrestricted Non Commercial use, distribution and ...
Tahseen Saddam Fandi Al-Mathkuri   +2 more
openalex   +2 more sources

Extraction RNA Protocol from Blood and Testis Tissue of Local Roosters

open access: yesTikrit Journal for Agricultural Sciences, 2021
The (one-step) method has become widely used to isolate total RNA from living organism samples and from different tissues. The aim of this study is to extract RNA from the blood and testicular tissue of male local chickens.
Ahmed Khalid   +3 more
doaj   +1 more source

Virtual in situs: Sequencing mRNA from cryo-sliced Drosophila embryos to determine genome-wide spatial patterns of gene expression [PDF]

open access: yes, 2013
Complex spatial and temporal patterns of gene expression underlie embryo differentiation, yet methods do not yet exist for the efficient genome-wide determination of spatial expression patterns during development.
Combs, Peter A., Eisen, Michael B.
core   +15 more sources

An optimized method to obtain high-quality RNA from different tissues in Lilium davidii var. unicolor

open access: yesScientific Reports, 2022
The high quality, yield and purity total RNA samples are essential for molecular experiments. However, harvesting high quality RNA in Lilium davidii var.
Chunlei Wang   +7 more
doaj   +1 more source

Home - About - Disclaimer - Privacy