Results 31 to 40 of about 463,109 (314)

Production of high-quality two-dimensional gel electrophoresis profile for marine medaka samples by using Trizol-based protein extraction approaches

open access: yesProteome Science, 2020
Background Marine medaka is among the most popular models of fish species for ecotoxicology and environmental research and proteomic studies are useful tools for understanding the molecular responses of medaka upon exposure to different environmental ...
Celia Sze-Nga Kwok   +5 more
doaj   +1 more source

Development of an alternative saliva test for diagnosis of SARS-CoV-2 using TRIzol: Adapting to countries with lower incomes looking for a large-scale detection program.

open access: yesPLoS ONE, 2021
The use of saliva for the diagnosis of SARS-CoV-2 has shown to be a good alternative to nasopharyngeal swabs (NPS), since it permits self-collection, avoids the exposure of healthy persons to infected patients, reduces waiting times, eliminates the need ...
Haydee Miranda-Ortiz   +15 more
doaj   +1 more source

How Does the Sample Preparation of Phytophthora infestans Mycelium Affect the Quality of Isolated RNA?

open access: yesCurrent Issues in Molecular Biology, 2023
RNA isolation from fungi and fungus-like organisms is not an easy task. Active endogenous RNases quickly hydrolyze RNA after the sample collection, and the thick cell wall prevents inhibitors from penetrating the cells.
Artemii A. Ivanov   +2 more
doaj   +1 more source

Comparison of methods for pre-processing, exosome isolation, and RNA extraction in unpasteurized bovine and human milk.

open access: yesPLoS ONE, 2021
Milk is a highly complex, heterogeneous biological fluid that contains non-nutritive, bioactive extracellular vesicles called exosomes. Characterization of milk-derived exosomes (MDEs) is challenging due to the lack of standardized methods that are ...
Sanoji Wijenayake   +6 more
doaj   +1 more source

CryoGrid-PIXUL-RNA: high throughput RNA isolation platform for tissue transcript analysis

open access: yesBMC Genomics, 2023
Background Disease molecular complexity requires high throughput workflows to map disease pathways through analysis of vast tissue repositories. Great progress has been made in tissue multiomics analytical technologies.
Scott A. Schactler   +10 more
doaj   +1 more source

MicroRNA-483 amelioration of experimental pulmonary hypertension. [PDF]

open access: yes, 2020
Endothelial dysfunction is critically involved in the pathogenesis of pulmonary arterial hypertension (PAH) and that exogenously administered microRNA may be of therapeutic benefit.
Bai, Liang   +22 more
core   +1 more source

Detection of MicroRNAs in Dried Serum Blots [PDF]

open access: yes, 2010
MicroRNAs are short RNAs which can be utilized as biomarkers for a variety of conditions. They are detectable in serum, and changes in the levels of circulating microRNAs have been associated with different diseases.
Reema Mallick   +2 more
core   +2 more sources

Increased Hematopoietic Extracellular RNAs and Vesicles in the Lung during Allergic Airway Responses. [PDF]

open access: yes, 2019
Extracellular RNAs (exRNAs) can be released by numerous cell types in vitro, are often protected within vesicles, and can modify recipient cell function. To determine how the composition and cellular sources of exRNAs and the extracellular vesicles (EVs)
Ansel, K Mark   +6 more
core   +1 more source

COMPARISON OF RNA EXTRACTION METHODS FOR Passiflora edulis SIMS LEAVES

open access: yesRevista Brasileira de Fruticultura, 2016
Functional genomic analyses require intact RNA; however, Passiflora edulis leaves are rich in secondary metabolites that interfere with RNA extraction primarily by promoting oxidative processes and by precipitating with nucleic acids. This study aimed to
ANNY CAROLYNE DA LUZ   +2 more
doaj   +1 more source

Ciprofloxacin enhances the stimulation of matrix metalloproteinase 3 expression by interleukin-1beta in human tendon-derived cells [PDF]

open access: yes, 2002
To determine whether the fluoroquinolone antibiotic ciprofloxacin, which can cause tendon pain and rupture in a proportion of treated patients, affects the expression of matrix metalloproteinases (MMPs) in human tendon-derived cells in culture.
Astrom   +16 more
core   +1 more source

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