Salmonella produce microRNA-like RNA fragment Sal-1 in the infected cells to facilitate intracellular survival. [PDF]
, 2017 Salmonella have developed a sophisticated machinery to evade immune clearance and promote survival in the infected cells. Previous studies were mostly focused on either bacteria itself or host cells, the interaction mechanism of host-pathogen awaits ...
Chen, Xi +11 more
core +2 more sources
FAST AND COST-EFFECTIVE RNA EXTRACTION FOR BCR-ABL QUANTIFICATION IN ONCO-HEMATOLOGY LABORATORIES
Hematology, Transfusion and Cell Therapy, 2023 Background: Chronic myeloid leukemia (CML) is a myeloproliferative neoplasm characterized by the presence of a BCR-ABL1 rearrangement; its detection and quantification by real-time quantitative PCR (RQ-PCR) plays a central role in CML diagnosis, therapy ...
C Pugliesi +4 more
doaj +1 more source
MicroRNA regulation of type 2 innate lymphoid cell homeostasis and function in allergic inflammation. [PDF]
, 2017 MicroRNAs (miRNAs) exert powerful effects on immunity through coordinate regulation of multiple target genes in a wide variety of cells. Type 2 innate lymphoid cells (ILC2s) are tissue sentinel mediators of allergic inflammation.
Ansel, K Mark +7 more
core +2 more sources
High-yield skeletal muscle protein recovery from TRIzol after RNA and DNA extraction
BioTechniques, 2020 Extraction of DNA, RNA and protein from the same sample would allow for direct comparison of genomic, transcriptomic and proteomic information. Commercially available kits exhibit poor protein yield and the TRIzol® reagent produces a protein pellet that ...
Yuan Wen +3 more
doaj +1 more source
Effect of Larval Nutrition on Maternal mRNA Contribution to the Drosophila Egg. [PDF]
, 2018 Embryonic development begins under the control of maternal gene products, mRNAs and proteins that the mother deposits into the egg; the zygotic genome is activated some time later. Maternal control of early development is conserved across metazoans. Gene
Cartwright, Emily L +3 more
core +2 more sources
RNA TRIzol isolation of Datura spp. v1
, 2021 We present the TRIzol protocol for RNA extraction. We employed leaf tissue from vegetative and reproductive plants from four solanaceous species of the genus Datura; D. stramonium, D. pruinosa, D. inoxia and D. wrightii.
Eunice Kariñho-Betancourt +1 more
openaire +1 more source
Temperatura de almacenamiento y cantidad de ARN extraído de plasma materno
Universitas Scientiarum, 2013 The discovery of nucleic acids in maternal plasma unlocked new possibilities for noninvasive prenatal diagnosis. However, the factors affecting the concentration of fetal RNA in maternal plasma samples are unspecified.
Paola Andrea Ayala Ramírez +2 more
doaj +1 more source
Double lysis: an integrative time-saving method yielding high-quality RNA from strawberry
Journal of Genetic Engineering and Biotechnology, 2020 Background The isolation of high-quality RNA from strawberry leaves and fruits is notoriously cumbersome. Both tissues are extremely rich in active secondary metabolites, as phenolics and pigments that inevitably perturb the isolation of RNA.
Mohamed Hazman +3 more
doaj +1 more source
RAE-1 ligands for the NKG2D receptor are regulated by E2F transcription factors, which control cell cycle entry. [PDF]
, 2012 The NKG2D stimulatory receptor expressed by natural killer cells and T cell subsets recognizes cell surface ligands that are induced on transformed and infected cells and facilitate immune rejection of tumor cells.
Hsiung, Benjamin +4 more
core +2 more sources
BioTechniques, 2020 The aim of this study was to assess two protocols for their capacities to simultaneously isolate RNA, mtDNA and ncDNA from mammalian cells. We compared the Invitrogen TRIzol-based method and Qiagen DNeasy columns, using the HepG2 cell line and human ...
Jean Nakhle +4 more
doaj +1 more source