Frontiers in Microbiology, 2017 Advances in high throughput, next generation sequencing technologies have allowed an in-depth examination of biological environments and phenomena, and are particularly useful for culture-independent microbial community studies.
Chijioke O. Elekwachi +5 more
doaj +1 more source
Macrophage migration inhibitory factor (MIF) family in arthropods : Cloning and expression analysis of two MIF and one D-dopachrome tautomerase (DDT) homologues in Mud crabs, Scylla paramamosain [PDF]
, 2016 Acknowledgements This research was supported by grants from the National Natural Science Foundation of China (Nos. 31172438 and U1205123), the Natural Science Foundation of Fujian Province (No.
Duan, Li-Peng +7 more
core +1 more source
Reverse chemical ecology approach for the identification of an oviposition attractant for Culex quinquefasciatus. [PDF]
, 2018 Pheromones and other semiochemicals play a crucial role in today's integrated pest and vector management strategies. These semiochemicals are typically discovered by bioassay-guided approaches.
Bhagavathy, Ganga +7 more
core +1 more source
Effect of Larval Nutrition on Maternal mRNA Contribution to the Drosophila Egg. [PDF]
, 2018 Embryonic development begins under the control of maternal gene products, mRNAs and proteins that the mother deposits into the egg; the zygotic genome is activated some time later. Maternal control of early development is conserved across metazoans. Gene
Cartwright, Emily L +3 more
core +2 more sources
RAE-1 ligands for the NKG2D receptor are regulated by E2F transcription factors, which control cell cycle entry. [PDF]
, 2012 The NKG2D stimulatory receptor expressed by natural killer cells and T cell subsets recognizes cell surface ligands that are induced on transformed and infected cells and facilitate immune rejection of tumor cells.
Hsiung, Benjamin +4 more
core +2 more sources
In vitro identification and in silico utilization of interspecies sequence similarities using GeneChip(® )technology [PDF]
, 2005 BACKGROUND: Genomic approaches in large animal models (canine, ovine etc) are challenging due to insufficient genomic information for these species and the lack of availability of corresponding microarray platforms. To address this problem, we speculated
Garcia, Joe GN +5 more
core +3 more sources
BioTechniques, 2020 The aim of this study was to assess two protocols for their capacities to simultaneously isolate RNA, mtDNA and ncDNA from mammalian cells. We compared the Invitrogen TRIzol-based method and Qiagen DNeasy columns, using the HepG2 cell line and human ...
Jean Nakhle +4 more
doaj +1 more source
Frontiers in Cell and Developmental Biology, 2021 The extraction of high-quality ribonucleic acid (RNA) from tissues and cells is a key step in many biological assays. Guanidinium thiocyanate-phenol-chloroform (AGPC) is a widely used and efficient method to obtain pure RNA from most tissues and cells ...
Martin Roszkowski, Isabelle M. Mansuy
doaj +1 more source
Application of high-throughput sequencing to whole rabies viral genome characterisation and its use for phylogenetic re-evaluation of a raccoon strain incursion into the province of Ontario [PDF]
, 2017 Raccoon rabies remains a serious public health problem throughout much of the eastern seaboard of North America due to the urban nature of the reservoir host and the many challenges inherent in multi-jurisdictional efforts to administer co-ordinated and ...
Biek, Roman +4 more
core +1 more source
TRIzol-based RNA Extraction: A Reliable Method for Gene Expression Studies [PDF]
Journal of Sciences, Islamic Republic of Iran, 2014 RNA extraction is a prerequisite technique for gene expression studies, analyzing the etiology and disease progression, treatment effects, as well as designing the diagnostic methods.
M. Pahlevan Kakhki
doaj