TRIzol-based RNA Extraction: A Reliable Method for Gene Expression Studies [PDF]
Journal of Sciences, Islamic Republic of Iran, 2014 RNA extraction is a prerequisite technique for gene expression studies, analyzing the etiology and disease progression, treatment effects, as well as designing the diagnostic methods.
M. Pahlevan Kakhki
doaj
Double lysis: an integrative time-saving method yielding high-quality RNA from strawberry
Journal of Genetic Engineering and Biotechnology, 2020 Background The isolation of high-quality RNA from strawberry leaves and fruits is notoriously cumbersome. Both tissues are extremely rich in active secondary metabolites, as phenolics and pigments that inevitably perturb the isolation of RNA.
Mohamed Hazman +3 more
doaj +1 more source
Frontiers in Cell and Developmental Biology, 2021 The extraction of high-quality ribonucleic acid (RNA) from tissues and cells is a key step in many biological assays. Guanidinium thiocyanate-phenol-chloroform (AGPC) is a widely used and efficient method to obtain pure RNA from most tissues and cells ...
Martin Roszkowski, Isabelle M. Mansuy
doaj +1 more source
Application of high-throughput sequencing to whole rabies viral genome characterisation and its use for phylogenetic re-evaluation of a raccoon strain incursion into the province of Ontario [PDF]
, 2017 Raccoon rabies remains a serious public health problem throughout much of the eastern seaboard of North America due to the urban nature of the reservoir host and the many challenges inherent in multi-jurisdictional efforts to administer co-ordinated and ...
Biek, Roman +4 more
core +1 more source
BioTechniques, 2020 The aim of this study was to assess two protocols for their capacities to simultaneously isolate RNA, mtDNA and ncDNA from mammalian cells. We compared the Invitrogen TRIzol-based method and Qiagen DNeasy columns, using the HepG2 cell line and human ...
Jean Nakhle +4 more
doaj +1 more source
A comparison of microRNA sequencing reproducibility and noise reduction using mirVana and TRIzol isolation methods [PDF]
International Journal of Computational Biology and Drug Design, 2014 MicroRNAseq (miRNAseq) is a form of RNAseq technology that has become an increasingly popular alternative to miRNA expression profiling. Unlike messenger RNA (mRNA), miRNA extraction can be difficult, and sequencing such small RNA can also be problematic.
Amma Bosompem +5 more
openaire +3 more sources
Cancer cell death induced by the NAD antimetabolite Vacor discloses the antitumor potential of SARM1
FEBS Letters, EarlyView.Vacor, a compound converted into the toxic metabolite Vacor adenine dinucleotide (VAD) by the nicotinamide salvage pathway enzymes NAMPT and NMNAT2, exhibits antitumor activity by inducing rapid and complete NAD depletion. We report that Vacor toxicity is limited to cell lines expressing high levels of SARM1, a NAD glycohydrolase.
Giuseppe Ranieri +5 more
wiley +1 more source
Phenol Based RNA Isolation is the Optimum Method for Study of Gene Expression in Human Urinary Sediment [PDF]
Journal of Sciences, Islamic Republic of Iran, 2019 Evaluation of gene expression in urinary sediment has been considered as a promising non-invasive approach for biomarker identification of kidney diseases.
Hootan Yazdani +3 more
doaj +1 more source
Evaluation of Spermatozoal Rna Extraction Methods [PDF]
, 2016 The study was aimed to compare different RNA isolation methods from sperm and testicular tissue. A suitable sperm purification technique is required as sperm RNA yield is strongly correlated with the number of sperms.
Das, Sovan
core
Increased Expression of Cell-Cell Signaling Genes by Stimulated Mononuclear Leukocytes in Patients with Previous Atherothrombotic Stroke A Whole Genome Expression Profile Study [PDF]
, 2009 Background/Aims: Inflammation plays an important role in atherosclerosis and stroke. Acute infections are recognized as trigger factors for ischemic stroke. Methods: In this whole genome expression profile study of 15 patients and 15 control subjects, we
Grau, Armin J. +12 more
core +1 more source