Results 71 to 80 of about 461,258 (325)

Temperatura de almacenamiento y cantidad de ARN extraído de plasma materno

Universitas Scientiarum, 2013
The discovery of nucleic acids in maternal plasma unlocked new possibilities for noninvasive prenatal diagnosis. However, the factors affecting the concentration of fetal RNA in maternal plasma samples are unspecified.
Paola Andrea Ayala Ramírez, Liz Ariane Gonzalez Ropero, Reggie García Robles   +2 more
doaj   +1 more source

Salmonella produce microRNA-like RNA fragment Sal-1 in the infected cells to facilitate intracellular survival. [PDF]

, 2017
Salmonella have developed a sophisticated machinery to evade immune clearance and promote survival in the infected cells. Previous studies were mostly focused on either bacteria itself or host cells, the interaction mechanism of host-pathogen awaits ...
Chen, Xi, Gu, Hongwei, Li, Donghai, Liang, Hongwei, LIU, Fenyong, Pan, Yi, Wang, Xiao-Ming, Zen, Ke, Zhang, Chen-Yu, Zhang, Tianfu, Zhao, Chihao, Zhao, Quan   +11 more
core   +2 more sources

RNA TRIzol isolation of Datura spp. v1

, 2021
We present the TRIzol protocol for RNA extraction. We employed leaf tissue from vegetative and reproductive plants from four solanaceous species of the genus Datura; D. stramonium, D. pruinosa, D. inoxia and D. wrightii.
Eunice Kariñho-Betancourt, rtapia not provided   +1 more
openaire   +1 more source

Double lysis: an integrative time-saving method yielding high-quality RNA from strawberry

Journal of Genetic Engineering and Biotechnology, 2020
Background The isolation of high-quality RNA from strawberry leaves and fruits is notoriously cumbersome. Both tissues are extremely rich in active secondary metabolites, as phenolics and pigments that inevitably perturb the isolation of RNA.
Mohamed Hazman, Farida Kabil, Shrouk Abd Elhamid, Peter Nick   +3 more
doaj   +1 more source

High Efficiency RNA Extraction From Sperm Cells Using Guanidinium Thiocyanate Supplemented With Tris(2-Carboxyethyl)Phosphine

Frontiers in Cell and Developmental Biology, 2021
The extraction of high-quality ribonucleic acid (RNA) from tissues and cells is a key step in many biological assays. Guanidinium thiocyanate-phenol-chloroform (AGPC) is a widely used and efficient method to obtain pure RNA from most tissues and cells ...
Martin Roszkowski, Isabelle M. Mansuy
doaj   +1 more source

Basal body stability and ciliogenesis requires the conserved component Poc1 [PDF]

, 2009
Centrioles are the foundation for centrosome and cilia formation. The biogenesis of centrioles is initiated by an assembly mechanism that first synthesizes the ninefold symmetrical cartwheel and subsequently leads to a stable cylindrical microtubule ...
Abal, Andersen, Avidor-Reiss, Azimzadeh, Badano, Bakowska, Balczon, Baron, Basmussen, Basto, Beisson, Berbari, Blachon, Bobinnec, Bobinnec, Broadhead, Brown, Bruns, Chad G. Pearson, Cheeseman, Culver, Dammermann, Dammermann, Daniel P.S. Osborn, Dupuis-Williams, Dutcher, Dutcher, Dutcher, Eddé, Eggenschwiler, Essner, Fry, Gaertig, Garreau de Loubresse, Gerdes, Graser, Gundersen, Hai, Hames, Hentschel, Hildebrandt, Hiraki, Jerka-Dziadosz, Kaczanowski, Keller, Keller, Kilburn, Kirkham, Kochanski, Le Clech, Leidel, Leidel, Leidel, Li, Liu, Mark Winey, Marshall, Marshall, Martinez-Campos, Matsuura, Million, Mottier-Pavie, Nakazawa, Nanney, Neuhauss, Orias, Paoletti, Pearson, Pearson, Pelletier, Peterkin, Philip L. Beales, Piperno, Rodrigues-Martins, Salisbury, Shang, Shang, Stemm-Wolf, Strnad, Thomas H. Giddings, Tobin, Tobin, Vladar, Westerfield, Wilkinson, Williams, Wloga, Woodland   +87 more
core   +2 more sources

Methods for simultaneous and quantitative isolation of mitochondrial DNA, nuclear DNA and RNA from mammalian cells

BioTechniques, 2020
The aim of this study was to assess two protocols for their capacities to simultaneously isolate RNA, mtDNA and ncDNA from mammalian cells. We compared the Invitrogen TRIzol-based method and Qiagen DNeasy columns, using the HepG2 cell line and human ...
Jean Nakhle, Tülin Özkan, Kateřina Lněničková, Philippe Briolotti, Marie-Luce Vignais   +4 more
doaj   +1 more source

Phenol Based RNA Isolation is the Optimum Method for Study of Gene Expression in Human Urinary Sediment [PDF]

Journal of Sciences, Islamic Republic of Iran, 2019
Evaluation of gene expression in urinary sediment has been considered as a promising non-invasive approach for biomarker identification of kidney diseases.
Hootan Yazdani, Shiva Kalantari, Mohsen Nafar, Mohammad Naji   +3 more
doaj   +1 more source

Targeting EZH2 reverses thyroid cell dedifferentiation and enhances iodide uptake in anaplastic thyroid cancer

FEBS Letters, EarlyView.
Anaplastic thyroid cancer (ATC) lacks iodide uptake ability due to MAPK activation increasing the expression of the histone methyltransferase EZH2, which represses thyroid differentiation genes (TDGs) such as the sodium iodide symporter (NIS). Dual inhibition of MAPK (U0126) and EZH2 (EPZ6438/Tazemetostat) reverses this mechanism, thus restoring TDG ...
Diego Claro de Mello, Marcella Maringolo Cristovão, Guilherme Henrique, Vinicius Gonçalves Rodrigues, Caroline Serrano‐Nascimento, Edna Teruko Kimura, Cesar Seigi Fuziwara   +6 more
wiley   +1 more source

Evaluation of Spermatozoal Rna Extraction Methods [PDF]

, 2016
The study was aimed to compare different RNA isolation methods from sperm and testicular tissue. A suitable sperm purification technique is required as sperm RNA yield is strongly correlated with the number of sperms.
Das, Sovan
core