Results 171 to 180 of about 18,345 (225)

Transcriptional Silencing of Multiple Genes in Trophozoites of Entamoeba histolytica

open access: yesPLoS Pathogens, 2006
In a previous work we described the transcriptional silencing of the amoebapore A (AP-A) gene (Ehap-a) of Entamoeba histolytica strain HM-1:IMSS. The silencing occurred following transfection with a plasmid containing a 5' upstream region (473 bp) of ...
Rivka Bracha   +2 more
exaly   +2 more sources

The importance of trophozoites in transmission of toxoplasmosis: Survival and pathogenity of toxoplasma gondii trophozoites in liquid media

Medical Hypotheses, 1978
It has been generally assumed that Toxoplasma gondii trophozoites cannot survive long outside the body, and that toxoplasmosis is transmitted by transplacental infection, cysts and oocysts. We tested the survival and pathogenicity of separated Toxoplasma trophozoites in saline, colostrum, and solutions of albumen and serum, and the penetration of ...
S.A. Räisänen, K.M. Saari
openaire   +2 more sources

Tubulin diversity in trophozoites of Giardia lamblia

Histochemistry and Cell Biology, 2003
Giardia lamblia is a diplomonad that parasitizes the small intestine of vertebrates. The trophozoite is multiflagellar and its cytoskeleton presents a complex organization of microtubular structures. One of these, the adhesive disk, consists of a microtubular spiral.
Loraine, Campanati   +5 more
openaire   +2 more sources

Metabolism of Trophozoites

1984
The protozoon Giardia lamblia is a member of the order Diplomonadida which includes a variety of binucleate flagellate parasites typically inhabiting the intestinal tract of mammals, birds, and reptiles (Kulda and Nohynkova, 1978). Each organism has eight flagella and a striated disc which might play a role in its attachment to enterocytes (Holberton ...
Donald G. Lindmark, Edward L. Jarroll
openaire   +1 more source

Cryopreservation of viable Giardia intestinalis trophozoites

Transactions of the Royal Society of Tropical Medicine and Hygiene, 1984
A technique is described for the cryopreservation of Giardia intestinalis trophozoites. The most satisfactory results were obtained when organisms were preserved with either 7.5 or 10% dimethyl sulphoxide (Me2SO) and cooled using a liquid nitrogen controlled freezer. Under these conditions more than 70% of organisms were motile after thawing.
Phillips, R. E.   +2 more
openaire   +5 more sources

Structure of the Trophozoite and Cyst

1984
In a letter to the Royal Society in 1681, Anton van Leeuwenhoek reported the microscopic observations of his own diarrheic stool (Dobell, 1920). Unfortunately, he made no drawings of the organism he saw, but his description is detailed and vivid enough to convince many that what he saw was Giardia duodenalis. His letter is also the first association of
Dennis E. Feely   +2 more
openaire   +1 more source

Transcriptional profiling of Entamoeba histolytica trophozoites

International Journal for Parasitology, 2005
We have developed an Entamoeba histolytica genomic DNA microarray and used it to develop a transcriptional profile of 1,971 E. histolytica (HM-1:IMSS) genes. The arrays accurately detected message abundance and 31-47% of amebic genes were expressed under standard tissue culture conditions (levels detectable by Northern blot analysis or RT-PCR ...
Ryan C, MacFarlane   +2 more
openaire   +2 more sources

Pyrimidine metabolism in Giardia lamblia trophozoites

Molecular and Biochemical Parasitology, 1982
The pyrimidine metabolism of Giardia lamblia trophozoites (Portland I strain) was studied using whole trophozoites and trophozoite homogenates. Pyrimidines and pyrimidine nucleosides were readily incorporated into nucleic acids. Orotic and aspartic acid incorporations were below the level of detection.
D G, Lindmark, E L, Jarroll
openaire   +2 more sources

INHIBITION OF IN VITRO ATTACHMENT OF GIARDIA TROPHOZOITES BY MUCIN

Journal of Parasitology, 2002
An enzyme-linked immunoabsorbent assay was developed to quantify the number of Giardia sp. trophozoites attached to a substratum. Trophozoites were allowed to attach for 5 or 10 min to untreated or modified substratum, or allowed to attach in the presence of cytoskeletal inhibitors.
Heidi, Roskens, Stanley L, Erlandsen
openaire   +2 more sources

Peroxidase Localization in Hartmanella Trophozoites

Proceedings, annual meeting, Electron Microscopy Society of America, 1971
Biochemical and differential centrifugation studies have demonstrated that the oxidative enzymes of Acanthamoeba sp. are localized in mitochondria and peroxisomes (microbodies). Although hartmanellid amoebae have been the subject of several electron microscopic studies, peroxisomes have not been described from these organisms or other protozoa ...
George E. Childs, Joseph H. Miller
openaire   +1 more source

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