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Tropoelastin purification: Improvements using enzyme inhibitors
Analytical Biochemistry, 1975Abstract The true monomerix size of porcine aortic tropoelastin is 72,000–74,000 daltons. The newly modified isolation procedure we describe gives meterial predominantly of this molecular size with improved yields. The protein is extremely susceptible to proteolysis and steps must be taken during isolation to prevent this occurrence.
L B, Sandberg, E, Bruenger, E G, Cleary
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Connective Tissue Research, 1991
Because tropoelastin is difficult to purify, most antibodies to elastin are raised against the insoluble form of the molecule. While these antibodies cross-react with tropoelastin, antigenic differences between insoluble and soluble elastin suggest that antibodies raised directly against tropoelastin might provide a more sensitive and specific reagent ...
I W, Prosser +6 more
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Because tropoelastin is difficult to purify, most antibodies to elastin are raised against the insoluble form of the molecule. While these antibodies cross-react with tropoelastin, antigenic differences between insoluble and soluble elastin suggest that antibodies raised directly against tropoelastin might provide a more sensitive and specific reagent ...
I W, Prosser +6 more
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Engineered Tropoelastin and Elastin-Based Biomaterials
2009Elastin is a key mammalian extracellular matrix protein that is critical to the elasticity, compliance, and resilience of a range of tissues including the vasculature, skin, and lung. In addition to providing mechanical integrity to tissues, elastin also has critical functions in the regulation of cell behavior and may help to modulate the coagulation ...
Steven G, Wise +2 more
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Tropoelastin Biosynthesis by Corneal Cells
Ophthalmologica, 2003The vertebrate cornea is an avascular tissue and does not contain elastic fibers. We tested the capacity of corneal epithelial cells and stromal keratocytes to synthesize tropoelastin. Explant cultures and cell cultures were obtained from these two cell types in standard culture conditions.
N. Isnard +3 more
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2012
A serum antibody, specific for tropoelastin was developed in rabbits. The tropoelastin antigen was extracted from the aortas of swine raised on a copper-deficient diet. The amino acid composition and molecular weight of the tropoelastin antigen was determined through amino acid analysis and disc electrophoresis.
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A serum antibody, specific for tropoelastin was developed in rabbits. The tropoelastin antigen was extracted from the aortas of swine raised on a copper-deficient diet. The amino acid composition and molecular weight of the tropoelastin antigen was determined through amino acid analysis and disc electrophoresis.
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Structural changes and facilitated association of tropoelastin
Archives of Biochemistry and Biophysics, 2003Circular dichroism studies of tropoelastin secondary structure show 4+/-1% alpha-helix in aqueous solutions. This is in contrast to the substantially higher amounts (up to 23+/-7%) of alpha-helix predicted by computer algorithms, which propose that regions of alpha-helix are limited to the alanine-rich cross-linking domains.
Lisa D, Muiznieks +2 more
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Ophthalmologica. Journal international d'ophtalmologie. International journal of ophthalmology. Zeitschrift fur Augenheilkunde, 2004
The vertebrate cornea is an avascular tissue and does not contain elastic fibers. We tested the capacity of corneal epithelial cells and stromal keratocytes to synthesize tropoelastin. Explant cultures and cell cultures were obtained from these two cell types in standard culture conditions.
N, Isnard +3 more
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The vertebrate cornea is an avascular tissue and does not contain elastic fibers. We tested the capacity of corneal epithelial cells and stromal keratocytes to synthesize tropoelastin. Explant cultures and cell cultures were obtained from these two cell types in standard culture conditions.
N, Isnard +3 more
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Cellular Expression of Tropoelastin mRNA Splice Variants
Matrix, 1992The primary transcript of tropoelastin is alternatively spliced into multiple mRNAs. The pattern and frequency of exon splicing is developmentally regulated, but the cellular profile of isoform expression within and among elastic tissues is not known.
W C, Parks +3 more
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Chick tropoelastin isolation and partial chemical characterization
Biochemical and Biophysical Research Communications, 1975Summary A method is described for the purification of tropoelastin from the aortas of copper-deficient chicks. The final product was suitable for sequencing. The first 35 N-terminal residues are presented. The N-terminal sequence is similar in many respects to the N-terminal sequence of porcine tropoelastin.
R B, Rucker +4 more
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Maßgeschneiderte Wundauflagen aus humanem Tropoelastin
Info Diabetologie, 2021openaire +1 more source