Results 111 to 120 of about 245,099 (255)

Sensing of Bacterial Type IV Secretion via the Unfolded Protein Response

mBio, 2013
Host cytokine responses to Brucella abortus infection are elicited predominantly by the deployment of a type IV secretion system (T4SS). However, the mechanism by which the T4SS elicits inflammation remains unknown. Here we show that translocation of the
Maarten F. de Jong, Tregei Starr, Maria G. Winter, Andreas B. den Hartigh, Robert Child, Leigh A. Knodler, Jan Maarten van Dijl, Jean Celli, Renée M. Tsolis   +8 more
doaj   +1 more source

COMP–PMEPA1 axis promotes epithelial‐to‐mesenchymal transition in breast cancer cells

Molecular Oncology, EarlyView.
This study reveals that cartilage oligomeric matrix protein (COMP) promotes epithelial‐to‐mesenchymal transition (EMT) in breast cancer. We identify PMEPA1 (protein TMEPAI) as a novel COMP‐binding partner that mediates EMT via binding to the TSP domains of COMP, establishing the COMP–PMEPA1 axis as a key EMT driver in breast cancer.
Konstantinos S. Papadakos, Gilar Gorji‐bahri, Lejla Gradjan, Julia Zajac, Kacper Gil, Yvonne Thokozile Nduku, Anna M. Blom   +6 more
wiley   +1 more source

Legionella Lem26 functions as an ATG8-activated effector that inhibits host autophagy

mBio
The intracellular pathogen Legionella pneumophila has evolved multiple effector proteins delivered into host cells by the Dot/Icm Type IVb secretion system that prevents recognition of the vacuole in which it resides by the host autophagy pathway.
Kevin R. Parducho, Zi Yang, Emily Guinn, Daniel Choi, Shanta Nag, Thomas J. Melia, Craig R. Roy   +6 more
doaj   +1 more source

Structural and Molecular Biology of Type IV Secretion Systems [PDF]

, 2017
Type IV secretion systems (T4SSs) are nanomachines that Gram-negative, Gram-positive bacteria, and some archaea use to transport macromolecules across their membranes into bacterial or eukaryotic host targets or into the extracellular milieu. They are the most versatile secretion systems, being able to deliver both proteins and nucleoprotein complexes ...
Bergé, C, Waksman, G, Terradot, L
openaire   +3 more sources

Pre‐analytical optimization of cell‐free DNA and extracellular vesicle‐derived DNA for mutation detection in liquid biopsies

Molecular Oncology, EarlyView.
Pre‐analytical handling critically determines liquid biopsy performance. This study defines practical best‐practice conditions for cell‐free DNA (cfDNA) and extracellular vesicle–derived DNA (evDNA), showing how processing time, storage conditions, tube type, and plasma input volume affect DNA integrity and mutation detection.
Jonas Dohmen, Lucrezia De Santis, Bret M. Stephens, Vincent Bernard, Jörg C. Kalff, Lara Braun, Phillipp Leyendecker, Johannes Röttgen, Daniel Weissinger, Anirban Maitra, Paola A. Guerrero, Alexander Semaan   +11 more
wiley   +1 more source

Metagenome diversity illuminates the origins of pathogen effectors

mBio
Recent metagenome-assembled genome (MAG) analyses have profoundly impacted Rickettsiology systematics. The discovery of basal lineages (novel families Mitibacteraceae and Athabascaceae) with predicted extracellular lifestyles exposed an evolutionary ...
Stephanie S. Lehman, Victoria I. Verhoeve, Timothy P. Driscoll, John F. Beckmann, Joseph J. Gillespie   +4 more
doaj   +1 more source

Type IV secretion systems: versatility and diversity in function. [PDF]

Cellular microbiology, 2010
Type IV secretion systems (T4SSs) are large protein complexes which traverse the cell envelope of many bacteria. They contain a channel through which proteins or protein-DNA complexes can be translocated. This translocation is driven by a number of cytoplasmic ATPases which might energize large conformational changes in the translocation complex.
Walldén, Karin, Rivera-Calzada, A., Waksman, Gabriel   +2 more
openaire   +2 more sources

Engineered extracellular vesicles enriched with the miR‐214/199a cluster enhance the efficacy of chemotherapy in ovarian cancer

Molecular Oncology, EarlyView.
Loss of the miR‐214/199a cluster is associated with recurrence in ovarian cancer. Engineered small extracellular vesicles (m214‐sEVs) elevate miR‐214‐3p/miR‐199a‐5p in tumor cells, suppress β‐catenin, TLR4, and YKT6 signaling, reprogram tumor‐derived sEV cargo, reduce chemoresistance and migration, and enhance carboplatin efficacy and survival in ...
Weida Wang, Ayesha Alvero, Yi Qin, Mingjin Wang, Alexandra Fox, Yanfeng Li, Michael Millman, Amy Kemper, Gil Mor, Xian Shuang Liu, Michael Chopp, Zheng Gang Zhang, Yi Zhang   +12 more
wiley   +1 more source

Brucella modulates secretory trafficking via multiple type IV secretion effector proteins.

PLoS Pathogens, 2013
The intracellular pathogenic bacterium Brucella generates a replicative vacuole (rBCV) derived from the endoplasmic reticulum via subversion of the host cell secretory pathway. rBCV biogenesis requires the expression of the Type IV secretion system (T4SS)
Sebenzile Myeni, Robert Child, Tony W Ng, John J Kupko, Tara D Wehrly, Stephen F Porcella, Leigh A Knodler, Jean Celli   +7 more
doaj   +1 more source

Type three secretion system-mediated escape of Burkholderia pseudomallei into the host cytosol is critical for the activation of NFκB. [PDF]

, 2014
BackgroundBurkholderia pseudomallei is the causative agent of melioidosis, a potentially fatal disease endemic in Southeast Asia and Northern Australia.
Chen, Isabelle Gek Joo, Chen, Yahua, Chiou, Pei-Yu, French, Christopher Todd, Gan, Yunn-Hwen, Miller, Jeff F, Sagullo, Enrico, Teh, Boon Eng, Teitell, Michael A, Wu, Ting-Hsiang   +9 more
core   +2 more sources