Results 111 to 120 of about 490,629 (332)

LDAcoop: Integrating non‐linear population dynamics into the analysis of clonogenic growth in vitro

Molecular Oncology, EarlyView.
Limiting dilution assays (LDAs) quantify clonogenic growth by seeding serial dilutions of cells and scoring wells for colony formation. The fraction of negative wells is plotted against cells seeded and analyzed using the non‐linear modeling of LDAcoop.
Nikko Brix, Daniel Samaga, Katharina Gehr, Benedek Dankó, Mohamed Schumann, Guido Drexler, Ahmed Alnatsha, Georg Beyer, Ujjwal Mahajan, Martin Selmansberger, Julia Mayerle, Claus Belka, Horst Zitzelsberger, Kirsten Lauber   +13 more
wiley   +1 more source

DNA Delivery and Genomic Integration into Mammalian Target Cells through Type IV A and B Secretion Systems of Human Pathogens

Frontiers in Microbiology, 2017
We explore the potential of bacterial secretion systems as tools for genomic modification of human cells. We previously showed that foreign DNA can be introduced into human cells through the Type IV A secretion system of the human pathogen Bartonella ...
Dolores L. Guzmán-Herrador, Samuel Steiner, Anabel Alperi, Coral González-Prieto, Craig R. Roy, Matxalen Llosa   +5 more
doaj   +1 more source

A subcutaneous adipose tissue-liver signalling axis controls hepatic gluconeogenesis. [PDF]

, 2015
The search for effective treatments for obesity and its comorbidities is of prime importance. We previously identified IKK-ε and TBK1 as promising therapeutic targets for the treatment of obesity and associated insulin resistance. Here we show that acute
Ahmadian, Maryam, Chang, Louise, Downes, Michael, Evans, Ronald M, Korytnaya, Evgenia, Krause, Danielle M, Liddle, Christopher, Lumeng, Carey N, Neidert, Adam, Oral, Elif A, Peng, Xiaoling, Poirier, BreAnne, Reilly, Shannon M, Saltiel, Alan R, Uhm, Maeran, Yu, Ruth T, Zamarron, Brian F   +16 more
core   +2 more sources

Helicobacter pylori Exploits a Unique Repertoire of Type IV Secretion System Components for Pilus Assembly at the Bacteria-Host Cell Interface

PLoS Pathogens, 2011
Colonization of the human stomach by Helicobacter pylori is an important risk factor for development of gastric cancer. The H. pylori cag pathogenicity island (cag PAI) encodes components of a type IV secretion system (T4SS) that translocates the ...
C. Shaffer, J. Gaddy, J. Loh, Elizabeth M. Johnson, S. Hill, E. Hennig, M. S. McClain, W. McDonald, T. Cover, N. Salama, Fred Hutchinson   +10 more
semanticscholar   +1 more source

SecReT4: a web-based bacterial type IV secretion system resource [PDF]

Nucleic Acids Research, 2012
SecReT4 (http://db-mml.sjtu.edu.cn/SecReT4/) is an integrated database providing comprehensive information of type IV secretion systems (T4SSs) in bacteria. T4SSs are versatile assemblages that promote genetic exchange and/or effector translocation with consequent impacts on pathogenesis and genome plasticity. T4SSs have been implicated in conjugation,
Bi, D., Liu, L., Tai, C., Deng, Z., Rajakumar, Kumar, Ou, H. Y.   +5 more
openaire   +3 more sources

Therapeutic strategies for MMAE‐resistant bladder cancer through DPP4 inhibition

Molecular Oncology, EarlyView.
We established monomethyl auristatin E (MMAE)‐resistant bladder cancer (BC) cell lines by exposure to progressively increasing concentrations of MMAE in vitro. RNA sequencing showed DPP4 expression was increased in MMAE‐resistant BC cells. Both si‐DPP4 and the DPP4 inhibitor sitagliptin suppressed the viability of MMAE‐resistant BC cells.
Gang Li, Shuichi Tatarano, Hirofumi Yoshino, Saeki Saito, Mitsuhiko Tominaga, Junya Arima, Ikumi Fukuda, Takashi Sakaguchi, Ryosuke Matsushita, Yasutoshi Yamada, Hideki Enokida   +10 more
wiley   +1 more source

Type IV secretion-dependent activation of host MAP kinases induces an increased proinflammatory cytokine response to Legionella pneumophila.

PLoS Pathogens, 2008
The immune system must discriminate between pathogenic and nonpathogenic microbes in order to initiate an appropriate response. Toll-like receptors (TLRs) detect microbial components common to both pathogenic and nonpathogenic bacteria, whereas Nod-like ...
Sunny Shin, Christopher L Case, Kristina A Archer, Catarina V Nogueira, Koichi S Kobayashi, Richard A Flavell, Craig R Roy, Dario S Zamboni   +7 more
doaj   +1 more source

Epithelial organization and cyst lumen expansion require efficient Sec13-Sec31-driven secretion [PDF]

, 2012
Epithelial morphogenesis is directed by interactions with the underlying extracellular matrix. Secretion of collagen and other matrix components requires efficient coat complex II (COPII) vesicle formation at the endoplasmic reticulum. Here, we show that
Hodgson, Lorna, Schmidt, Katy, Stephens, David J, Townley, Anna K   +3 more
core   +2 more sources

Secretion of Chromosomal DNA by the Neisseria gonorrhoeae Type IV Secretion System [PDF]

, 2017
Approximately 80% of Neisseria gonorrhoeae and 17.5% of Neisseria meningitidis clinical isolates carry a ~59 kb genomic island known as the gonococcal genetic island (GGI). About half of the GGI consists of genes encoding a type IV secretion system (T4SS), and most of these genes are clustered in a ~28 kb region at one end of the GGI.
Melanie M, Callaghan, Jan-Hendrik, Heilers, Chris, van der Does, Joseph P, Dillard   +3 more
openaire   +2 more sources

Peroxidasin enables melanoma immune escape by inhibiting natural killer cell cytotoxicity

Molecular Oncology, EarlyView.
Peroxidasin (PXDN) is secreted by melanoma cells and binds the NK cell receptor NKG2D, thereby suppressing NK cell activation and cytotoxicity. PXDN depletion restores NKG2D signaling and enables effective NK cell–mediated melanoma killing. These findings identify PXDN as a previously unrecognized immune evasion factor and a potential target to improve
Hsu‐Min Sung, David Bickel, Lena C. M. Krause, Daria Ezeriņa, Christian Ickes, Julian Wojtachnia, Christine S. Gibhardt, Magdalena Shumanska, Khadija Wahni, Andrea Paluschkiwitz, Julia Malo Pueyo, Ekaterina Baranova, Wim Vranken, Hedwig Stanisz, Ioana Stejerean‐Todoran, Michael P. Schön, Joris Messens, Ivan Bogeski   +17 more
wiley   +1 more source