Results 31 to 40 of about 33,989 (317)
The CaVβ Subunit Protects the I-II Loop of the Voltage-gated Calcium Channel CaV2.2 from Proteasomal Degradation but Not Oligoubiquitination. [PDF]
, 2016 CaVβ subunits interact with the voltage-gated calcium channel CaV2.2 on a site in the intracellular loop between domains I and II (the I-II loop). This interaction influences the biophysical properties of the channel and leads to an increase in its ...
Dolphin, AC, Page, KM, Rothwell, SW
core +1 more source
Ubiquitin in Motion: Structural Studies of the Ubiquitin-Conjugating Enzyme∼Ubiquitin Conjugate [PDF]
Biochemistry, 2011 Ubiquitination of proteins provides a powerful and versatile post-translational signal in the eukaryotic cell. The formation of a thioester bond between ubiquitin (Ub) and the active site of a ubiquitin-conjugating enzyme (E2) is critical for the transfer of Ub to substrates. Assembly of a functional ubiquitin ligase (E3) complex poised for Ub transfer
Rachel E. Klevit +4 more
openaire +3 more sources
Myosin VI Contains a Compact Structural Motif that Binds to Ubiquitin Chains
Cell Reports, 2016 Myosin VI is critical for cargo trafficking and sorting during early endocytosis and autophagosome maturation, and abnormalities in these processes are linked to cancers, neurodegeneration, deafness, and hypertropic cardiomyopathy.
Fahu He +9 more
doaj +1 more source
Rapid E2-E3 Assembly and Disassembly Enable Processive Ubiquitylation of Cullin-RING Ubiquitin Ligase Substrates [PDF]
, 2009 Degradation by the ubiquitin-proteasome system requires assembly of a polyubiquitin chain upon substrate. However, the structural and mechanistic features that enable template-independent processive chain synthesis are unknown.
Deshaies, Raymond J. +4 more
core +3 more sources
Experimental and Molecular Medicine, 2018 Experimental & molecular medicine Researchers may have found a new target for treatment of multiple myeloma (MM), a common type of blood cancer.
Lili Zhou +7 more
doaj +1 more source
Frontiers in Physiology, 2018 Structural and functional alterations of mitochondria are intimately linked to a wide array of medical conditions. Many factors are involved in the regulation of mitochondrial function, including cytokines, chaperones, chemokines, neurosteroids, and ...
Victoria O. Polyakova +10 more
doaj +1 more source
A novel synthetic chemistry approach to linkage-specific ubiquitin conjugation. [PDF]
, 2015 Ubiquitination is of great importance as the post-translational modification of proteins with ubiquitin, or ubiquitin chains, facilitates a number of vital cellular processes.
Caddick, S +4 more
core +1 more source
, 2011 In this chapter, we describe a method for detecting the ubiquitination status of G protein-coupled receptors (GPCRs). This involves co-expression of a GPCR with an epitope-tagged ubiquitin construct in a -heterologous mammalian expression system. Stimulus-dependent modification of the GPCR by -ubiquitin is detected by immunoprecipitation and subsequent
Adriano Marchese, Adriana Caballero
openaire +3 more sources
Frontiers in Microbiology, 2018 Ubiquitination is an essential protein modification in eukaryotic cells, which is reversible. Deubiquitinating enzymes (DUBs) catalyze deubiquitination process to reverse ubiquitination, maintain ubiquitin homeostasis or promote protein degradation by ...
Zhao Wang +5 more
doaj +1 more source
Ubiquitin- and ATP-dependent unfoldase activity of P97/VCP•NPLOC4•UFD1L is enhanced by a mutation that causes multisystem proteinopathy [PDF]
, 2017 p97 is a “segregase” that plays a key role in numerous ubiquitin (Ub)-dependent pathways such as ER-associated degradation. It has been hypothesized that p97 extracts proteins from membranes or macromolecular complexes to enable their proteasomal ...
Blythe, Emily E. +3 more
core +2 more sources