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Viral nanotechnology enables new possibilities for gene therapies and vaccines. However, the manufacturing of viral vectors lacks a satisfactory production capable method for purification of full capsids.
Emanuel J. Hubmann+3 more
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Comparison of Two Different Methods for the Extraction of Outer Membrane Vesicles from the Bordetella pertussis as a Vaccine Candidate [PDF]
Despite the availability of a vaccine, pertussis is still a worldwide health problem. Outer membrane vesicles (OMVs) in gram-negative bacteria can stimulate the immune system due to several outer membrane proteins and are very good candidates in vaccine ...
M. S Soltani+4 more
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A Miniature Sucrose Gradient for Polysome Profiling
Polysome profiling by sucrose density gradient centrifugation is commonly used to study the overall degree of translation (messenger RNA to protein synthesis). Traditionally, the method begins with synthesis of a 5–10 mL sucrose gradient onto which 0.5–1
Ansul Lokdarshi, Albrecht von Arnim
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Background: Mesenchymal stem cells (MSCs)-derived exosomes can function similar to MSCs in repairing damaged tissues and modulating immune responses. They are considered as an effective tool in regenerative medicine as well as autoimmune diseases and ...
Fataneh Tavasolian+3 more
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Extracellular vesicles (EVs) are nano-sized vesicles surrounded by a lipid bilayer and released into the extracellular milieu by most of cells. Although various EV isolation methods have been established, most of the current methods isolate EVs with ...
Dongsic Choi+6 more
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Diffusion of solvent from a cast cellulose acetate solution during the formation of skinned membranes [PDF]
The transport of solvent out of a cast cellulose acetate (CA) solution into the coagulation bath during membrane formation is treated as a diffusion process.
Altena, F.W.+4 more
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Overview of Methods for the Direct Molar Mass Determination of Cellulose
The purpose of this article is to provide the reader with an overview of the methods used to determine the molecular weights of cellulose. Methods that employ direct dissolution of the cellulose polymer are described; hence methods for investigating the ...
Josua Timotheus Oberlerchner+2 more
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Molecular flexibility of citrus pectins by combined sedimentation and viscosity analysis [PDF]
The flexibility/rigidity of pectins plays an important part in their structure-function relationship and therefore on their commercial applications in the food and biomedical industries.
Alan Smith+47 more
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Exploiting double exchange Diels-Alder cycloadditions for immobilization of peptide nucleic acids on gold nanoparticles [PDF]
The generation of PNA-decorated gold nanoparticles (AuNPs) has revealed to be more difficult as compared to the generation of DNA-functionalized ones. The less polar nature of this artificial nucleic acid system and the associated tendency of the neutral
Cadoni, Enrico+4 more
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Immunochemical studies on human plasma lipoproteins [PDF]
Some years ago, a series of human serum lipoproteins, distinguishable by their hydrated densities and lipid-protein ratios were recognized and isolated by ultracentrifugal techniques (1).
Aladjem, Frederick+2 more
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