Results 11 to 20 of about 70,242 (336)

Characterization of physical properties of tissue factor–containing microvesicles and a comparison of ultracentrifuge-based recovery procedures

open access: yesJournal of Extracellular Vesicles, 2014
Microvesicles were isolated from the conditioned media of 3 cell lines (MDA-MB-231, AsPC-1 and A375) by ultracentrifugation at a range of relative centrifugal forces, and the tissue factor (TF) protein and activity, microvesicle number, size distribution
Camille Ettelaie   +3 more
doaj   +2 more sources

Comparison of Two Different Methods for the Extraction of Outer Membrane Vesicles from the Bordetella pertussis as a Vaccine Candidate [PDF]

open access: yesArchives of Razi Institute, 2021
Despite the availability of a vaccine, pertussis is still a worldwide health problem. Outer membrane vesicles (OMVs) in gram-negative bacteria can stimulate the immune system due to several outer membrane proteins and are very good candidates in vaccine ...
M. S Soltani   +4 more
doaj   +1 more source

Reincarnation of the Analytical Ultracentrifuge: Emerging Opportunities for Nanomedicine.

open access: yesAnalytical Chemistry, 2021
The analytical ultracentrifuge (AUC) and the modern field of analytical ultracentrifugation found its inception approximately a century ago. We highlight the scope of its major experimental opportunities as a transport-based method, contemporary and up ...
Olenka Jibaja Valderrama, I. Nischang
semanticscholar   +1 more source

A Miniature Sucrose Gradient for Polysome Profiling

open access: yesBio-Protocol, 2023
Polysome profiling by sucrose density gradient centrifugation is commonly used to study the overall degree of translation (messenger RNA to protein synthesis). Traditionally, the method begins with synthesis of a 5–10 mL sucrose gradient onto which 0.5–1
Ansul Lokdarshi, Albrecht von Arnim
doaj   +1 more source

Isolation and Characterization of Mesenchymal Stem Cells-Derived Exosomes by Method of Ultracentrifuge and Polyethylene Glycol

open access: yesمجله دانشکده پزشکی اصفهان, 2020
Background: Mesenchymal stem cells (MSCs)-derived exosomes can function similar to MSCs in repairing damaged tissues and modulating immune responses. They are considered as an effective tool in regenerative medicine as well as autoimmune diseases and ...
Fataneh Tavasolian   +3 more
doaj   +1 more source

Quantitative proteomic analysis of trypsin-treated extracellular vesicles to identify the real-vesicular proteins

open access: yesJournal of Extracellular Vesicles, 2020
Extracellular vesicles (EVs) are nano-sized vesicles surrounded by a lipid bilayer and released into the extracellular milieu by most of cells. Although various EV isolation methods have been established, most of the current methods isolate EVs with ...
Dongsic Choi   +6 more
doaj   +1 more source

Exploiting double exchange Diels-Alder cycloadditions for immobilization of peptide nucleic acids on gold nanoparticles [PDF]

open access: yes, 2020
The generation of PNA-decorated gold nanoparticles (AuNPs) has revealed to be more difficult as compared to the generation of DNA-functionalized ones. The less polar nature of this artificial nucleic acid system and the associated tendency of the neutral
Cadoni, Enrico   +4 more
core   +2 more sources

Overview of Methods for the Direct Molar Mass Determination of Cellulose

open access: yesMolecules, 2015
The purpose of this article is to provide the reader with an overview of the methods used to determine the molecular weights of cellulose. Methods that employ direct dissolution of the cellulose polymer are described; hence methods for investigating the ...
Josua Timotheus Oberlerchner   +2 more
doaj   +1 more source

Immunochemical studies on human plasma lipoproteins [PDF]

open access: yes, 1957
Some years ago, a series of human serum lipoproteins, distinguishable by their hydrated densities and lipid-protein ratios were recognized and isolated by ultracentrifugal techniques (1).
Aladjem, Frederick   +2 more
core   +1 more source

Lipemia Interferences in Biochemical Tests, Investigating the Efficacy of Different Removal Methods in comparison with Ultracentrifugation as the Gold Standard

open access: yesJournal of Analytical Methods in Chemistry, 2020
Introduction. As a common interferer in clinical chemistry, lipemic specimens could be a source of significant analytical errors. Ultracentrifugation has been by far the only reliable, but an unavailable and expensive, method to eliminate the lipemic ...
Neda Soleimani   +2 more
doaj   +1 more source

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