Results 11 to 20 of about 68,271 (373)

Characterization of physical properties of tissue factor–containing microvesicles and a comparison of ultracentrifuge-based recovery procedures

open access: yesJournal of Extracellular Vesicles, 2014
Microvesicles were isolated from the conditioned media of 3 cell lines (MDA-MB-231, AsPC-1 and A375) by ultracentrifugation at a range of relative centrifugal forces, and the tissue factor (TF) protein and activity, microvesicle number, size distribution
Camille Ettelaie   +3 more
doaj   +2 more sources

3D-Printing for Analytical Ultracentrifugation

open access: yesPLOS ONE, 2016
Analytical ultracentrifugation (AUC) is a classical technique of physical biochemistry providing information on size, shape, and interactions of macromolecules from the analysis of their migration in centrifugal fields while free in solution.
Desai, Abhiksha   +4 more
core   +7 more sources

Reincarnation of the Analytical Ultracentrifuge: Emerging Opportunities for Nanomedicine

open access: hybridAnalytical Chemistry, 2021
The analytical ultracentrifuge (AUC) and the modern field of analytical ultracentrifugation found its inception approximately a century ago. We highlight the scope of its major experimental opportunities as a transport-based method, contemporary and up ...
Olenka Jibaja Valderrama, Ivo Nischang
openalex   +2 more sources

Improving the thermal, radial, and temporal accuracy of the analytical ultracentrifuge through external references [PDF]

open access: greenAnalytical Biochemistry, 2013
Rodolfo Ghirlando   +7 more
openalex   +2 more sources

Analysis of data from the analytical ultracentrifuge by nonlinear least-squares techniques

open access: bronzeBiophysical Journal, 1981
Michael L. Johnson   +3 more
openalex   +2 more sources

The Beams Ultracentrifuge [PDF]

open access: bronzeNature, 1936
AN ultracentrifuge designed by Beams has been in operation in this laboratory for the past year. From information published1,2,3,4, we assumed the forces to be of the order of 5 × 105 times gravity when driven by air at about 80 lb. pressure. On subsequent measurement we find that the maximum force developed is 4 × 106 times gravity.
WILLIAM CUSACK FAHIE, Ralph H. Brown
openalex   +3 more sources

Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge.

open access: yesClinical Chemistry, 1972
A method for estimating the cholesterol content of the serum low-density lipoprotein fraction (Sf0-20) is presented. The method involves measurements of fasting plasma total cholesterol, triglyceride, and high-density lipoprotein cholesterol ...
W. T. Friedewald   +2 more
semanticscholar   +1 more source

Recent Advances in the Analysis of Macromolecular Interactions Using the Matrix-Free Method of Sedimentation in the Analytical Ultracentrifuge

open access: yesBiology, 2015
Sedimentation in the analytical ultracentrifuge is a matrix free solution technique with no immobilisation, columns, or membranes required and can be used to study self-association and complex or “hetero”-interactions, stoichiometry, reversibility and ...
Stephen E. Harding   +5 more
doaj   +1 more source

Lipemia Interferences in Biochemical Tests, Investigating the Efficacy of Different Removal Methods in comparison with Ultracentrifugation as the Gold Standard

open access: yesJournal of Analytical Methods in Chemistry, 2020
Introduction. As a common interferer in clinical chemistry, lipemic specimens could be a source of significant analytical errors. Ultracentrifugation has been by far the only reliable, but an unavailable and expensive, method to eliminate the lipemic ...
Neda Soleimani   +2 more
doaj   +1 more source

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