Dynamic protein interactions probed by NMR spectroscopy
Abstract Dynamic protein–protein interactions are essential for diverse cellular processes but often evade structural characterization due to their transient, heterogeneous, and disordered nature. This review focuses on how nuclear magnetic resonance (NMR) spectroscopy can provide detailed, residue‐level insights into these complex interactions.
Jaeseok Lee, Jung Ho Lee
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Protocol for the isolation of human neutrophil-derived extracellular vesicles for characterization and functional experimentation. [PDF]
Richter R+9 more
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Analytical Ultracentrifuge Studies of a Cytokine, Soluble Receptor Interaction: Comparison of Simulations and Experiments [PDF]
Robert T. Wright+3 more
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The study reveals that microglial activation significantly contributes to neuroinflammation following cerebral ischemia‐reperfusion injury. It demonstrates that utilizing GelMA hydrogel for three‐dimensional culture of bone marrow‐derived mesenchymal stem cells (BMSCs) enhances the efficacy of BMSC‐derived exosomes in regulating microglial polarization
Yuming Li+13 more
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Improving AAV Production Yield and Quality for Different Serotypes Using Distinct Processing Methods. [PDF]
Khaparde A+4 more
europepmc +1 more source
A double‐positive surface marker detection approach was developed to conduct exosome surface protein detection in serum samples. It reaches single‐exosome detection, and over 300 exosome protein parameters are analyzed. The diagnostic accuracy achieved 90% in differentiation of high‐grade serous/serous ovarian carcinoma patients, benign patients, and ...
Jiaoyan Qiu+7 more
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Protocol for in vivo tracking of lipoproteins in developing oocytes of multiple species of Chagas disease vectors using fluorescent probes. [PDF]
Fruttero LL+3 more
europepmc +1 more source
Exosome as bioactive nanovesicle for diagnostic and therapeutic applications in periodontitis
This review introduces the mechanism that exosomes participate in the pathogenesis of periodontitis and their potential as biomarkers for early diagnosis and summarizes the application of cell‐ or plant‐derived exosomes or engineered exosomes in periodontitis or periodontal regeneration while proposing the perspective of translational application of ...
Yu Wang+6 more
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Immunological Studies on Ultracentrifuged-Fractions from Sal. Enteritidis
Tatsuji Yoshioka
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Continuous purification of a parvovirus using two aqueous two‐phase extraction steps
Abstract Aqueous two‐phase systems (ATPS) are a liquid–liquid extraction method that offers low‐cost, continuous‐adaptable virus purification. A two‐step ATPS using polyethylene glycol (PEG) and sodium citrate that recovered 66% of infectious porcine parvovirus with 2.0 logs of protein removal and 1.0 logs of DNA removal in batch has now been run ...
Natalie M. Nold+11 more
wiley +1 more source