Results 191 to 200 of about 68,271 (373)

Dynamic protein interactions probed by NMR spectroscopy

open access: yesBulletin of the Korean Chemical Society, EarlyView.
Abstract Dynamic protein–protein interactions are essential for diverse cellular processes but often evade structural characterization due to their transient, heterogeneous, and disordered nature. This review focuses on how nuclear magnetic resonance (NMR) spectroscopy can provide detailed, residue‐level insights into these complex interactions.
Jaeseok Lee, Jung Ho Lee
wiley   +1 more source

Protocol for the isolation of human neutrophil-derived extracellular vesicles for characterization and functional experimentation. [PDF]

open access: yesSTAR Protoc
Richter R   +9 more
europepmc   +1 more source

3D‐cultured BMSC exosomes improve cerebral ischemia/reperfusion injury‐induced neuronal apoptosis by regulating the microglia polarization

open access: yesBMEMat, EarlyView.
The study reveals that microglial activation significantly contributes to neuroinflammation following cerebral ischemia‐reperfusion injury. It demonstrates that utilizing GelMA hydrogel for three‐dimensional culture of bone marrow‐derived mesenchymal stem cells (BMSCs) enhances the efficacy of BMSC‐derived exosomes in regulating microglial polarization
Yuming Li   +13 more
wiley   +1 more source

High‐throughput exosomes analysis for ovarian cancer screening based on a double‐positive surface marker detection biochip

open access: yesBMEMat, EarlyView.
A double‐positive surface marker detection approach was developed to conduct exosome surface protein detection in serum samples. It reaches single‐exosome detection, and over 300 exosome protein parameters are analyzed. The diagnostic accuracy achieved 90% in differentiation of high‐grade serous/serous ovarian carcinoma patients, benign patients, and ...
Jiaoyan Qiu   +7 more
wiley   +1 more source

Exosome as bioactive nanovesicle for diagnostic and therapeutic applications in periodontitis

open access: yesBMEMat, EarlyView.
This review introduces the mechanism that exosomes participate in the pathogenesis of periodontitis and their potential as biomarkers for early diagnosis and summarizes the application of cell‐ or plant‐derived exosomes or engineered exosomes in periodontitis or periodontal regeneration while proposing the perspective of translational application of ...
Yu Wang   +6 more
wiley   +1 more source

Continuous purification of a parvovirus using two aqueous two‐phase extraction steps

open access: yesBiotechnology Progress, EarlyView.
Abstract Aqueous two‐phase systems (ATPS) are a liquid–liquid extraction method that offers low‐cost, continuous‐adaptable virus purification. A two‐step ATPS using polyethylene glycol (PEG) and sodium citrate that recovered 66% of infectious porcine parvovirus with 2.0 logs of protein removal and 1.0 logs of DNA removal in batch has now been run ...
Natalie M. Nold   +11 more
wiley   +1 more source

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