Results 201 to 210 of about 70,242 (336)

Systemic Clodronate Liposome Treatment for Depletion of Blood-Contacting Phagocytes and Investigation of Their Role in the Control of Bunyavirus Viremia. [PDF]

Methods Mol Biol
Ander SE, Davenport BJ, Evans AB, Morrison TE.   +3 more
europepmc   +1 more source

Scalable production and biophysical characterization of an enzyme cocktail derived from human red blood cells

Biotechnology Progress, EarlyView.
Abstract Red blood cells (RBCs) play a critical role in oxygen and carbon dioxide transport, which is facilitated by RBC‐encapsulated hemoglobin (Hb) and carbonic anhydrase (CA). In addition, RBCs are constantly exposed to oxidative stress due to the intracellular reactive oxygen species (ROS) generated during Hb auto‐oxidation.
Mohd Asim Khan, Griffin J. Beyer, Naomi Goosby, Lilly Ortiz, Andre F. Palmer   +4 more
wiley   +1 more source

Performance of large virus removal filters during AAV processing: Influence of flux and process disruptions

Biotechnology Progress, EarlyView.
Abstract As adeno‐associated viral vectors (AAV) continue to advance through the clinical pipeline, effective downstream purification strategies must be developed to ensure bulk drug purity and safety. AAV are produced within mammalian cells, bringing forth risks associated with viral contamination.
Akshay S. Chaubal, Asingsa W. Arachchige, Annabelle J. Zahn, S. Ranil Wickramasinghe, Xianghong Qian, Andrew L. Zydney   +5 more
wiley   +1 more source

Exosomal Protein Markers as Potential Non-Invasive Biomarkers for Colorectal Cancer. [PDF]

Int J Mol Sci
Skrzypek M, Piotrzkowska D, Adamkiewicz J, Prusisz M, Bijak M, Gornik L, Dziki L, Majsterek I.   +7 more
europepmc   +1 more source

Protein aggregation in C-phycocyanin. Studies at very low concentrations with the photoelectric scanner of the ultracentrifuge.

Biochemical Journal, 1971
R. Maccoll, J. J. Lee, D. Berns
semanticscholar   +1 more source

Purification, Fluorescent Labeling, and Detyrosination of Mammalian Cell Tubulin for Biochemical Assays

Cytoskeleton, EarlyView.
ABSTRACT Microtubules play essential roles in numerous cellular processes. All microtubules are built from the protein tubulin, yet individual microtubules can differ spatially and temporally due to their tubulin isotype composition and post‐translational modifications (PTMs).
Ezekiel C. Thomas, Yang Yue, Morgan L. Pimm, Takashi Hotta, Ryoma Ohi, Kristen J. Verhey   +5 more
wiley   +1 more source

Human plasma-derived eNAMPT-containing extracellular vesicles promote NAD⁺ biosynthesis and thermogenesis in mice. [PDF]

NPJ Aging
Yoshioka K, Sugimoto T, Oyabu M, Ito N, Kodama A, Kamei Y, Imai SI.   +6 more
europepmc   +1 more source

Expression, Purification, and Microscopy‐Based Assays for Engineered Recombinant Tyrosinated, Detyrosinated, and Δ2 Human Tubulin

Cytoskeleton, EarlyView.
ABSTRACT Microtubules are noncovalent polymers assembled from α/β tubulin dimers. Their structure, dynamics and interaction with effectors are regulated through the expression of diverse tubulin isotypes and chemically diverse posttranslational modifications, also known as the “tubulin code.” Understanding the biophysical correlates between tubulin ...
Jiayi Chen, Agnieska Szyk, Antonina Roll‐Mecak   +2 more
wiley   +1 more source

Proteomic analysis revealed the potential usefulness of faecal extracellular vesicles in colorectal cancer diagnosis. [PDF]

Sci Rep
Murakami Y, Sakamaki N, Ohiro Y.
europepmc   +1 more source

General solution to the inverse problem of the differential equation of the ultracentrifuge.

Proceedings of the National Academy of Sciences of the United States of America, 1981
G. P. Todd, R. Haschemeyer
semanticscholar   +1 more source