Results 251 to 260 of about 68,271 (373)

Construction of Reporter Phage T4::Nluc and Its Application in the Detection of Escherichia coli in Urinary Tract Infections

open access: yesiLABMED, EarlyView.
A reporter phage T4::Nluc was constructed using the CRISPR/Cas9 system combined with homologous recombination and was applied for the detection of clinical Escherichia coli in urinary tract infections (UTIs). T4::Nluc exhibited superior sensitivity and specificity, and had a short detection time, making it a powerful tool for diagnosing UTIs.
Zhiyun Hao   +6 more
wiley   +1 more source

Organoid‐tissue extracellular vesicles

open access: yesInterdisciplinary Medicine, EarlyView.
Abstract Extracellular vesicles (EVs) are lipid bilayer structures secreted by cells that act as intercellular messengers. Tissue‐derived EVs (TEVs), harvested from the tissue interstitium, directly reflect the actual physiological or pathological state of the tissue microenvironment.
Han Liu   +6 more
wiley   +1 more source

Generation of Conformation-Specific Monoclonal Antibodies for Integral Membrane Proteins. [PDF]

open access: yesCurr Protoc
Sheldon N   +7 more
europepmc   +1 more source

Bulk polymerization of cholesteryl acrylate [PDF]

open access: yes, 1970
Bantjes, A.   +3 more
core   +3 more sources

Chinese herbal medicine‐derived extracellular vesicle‐like particles: Therapeutic potential and future research approaches

open access: yesInterdisciplinary Medicine, EarlyView.
This article evaluates the therapeutic potential of extracellular vesicle‐like particles derived from Chinese herbal medicine (CHM‐EVLP) for a range of diseases. It underscores the significance of traditional Chinese medicine in the development of innovative formulations.
Ying‐qi Cao   +6 more
wiley   +1 more source

Capture of adeno‐associated viruses from clarified lysate by continuous flow ultracentrifugation: a comparative study of iodixanol and sucrose gradients

open access: yesJournal of Chemical Technology &Biotechnology, EarlyView.
Abstract BACKGROUND Adeno‐Associated Virus vectors are widely used in gene therapy due to their safety and efficacy. Despite the interest in their therapeutical application, AAV purification remains a significant challenge. Density gradient ultracentrifugation using cesium chloride, sucrose, or iodixanol gradients are common methods for AAV ...
Sabrina Leigheb   +6 more
wiley   +1 more source

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