Results 21 to 30 of about 70,242 (336)

The Buoyant Behavior of Viral and Bacterial DNA in Alkaline CsCl [PDF]

open access: yes, 1963
In equilibrium density gradient centrifugation, the banding polymer species is electrically neutral. The banding species for a negative polyelectrolyte with a polyanion P_(n)^(-z)n (where n is the degree of polymerization, and z the titration charge per
Davidson, Norman   +3 more
core   +1 more source

Three method-combination protocol for improving purity of extracellular vesicles [PDF]

open access: yes, 2020
Extracellular vesicles (EVs) are nanosized structures able to carry proteins, lipids and genetic material from one cell to another with critical implications in intercellular communication mechanisms.
Giamas, Georgios   +3 more
core   +1 more source

Factors affecting the integrity of high density lipoproteins in the ultracentrifuge

open access: yesJournal of Lipid Research, 1982
Because of reported losses of apolipoproteins from high density lipoproteins during ultracentrifugation, we studied several factors that could affect the integrity of these lipoprotein complexes.
S T Kunitake, J P Kane
doaj   +1 more source

Bismuth ions are metabolized into autometallographic traceable bismuth-sulphur quantum dots

open access: yesEuropean Journal of Histochemistry, 2009
Bismuth – sulphur quantum dots can be silver enhanced by autometallography (AMG). In the present study, autometallographic silver enhanced bismuth-sulphur nanocrystals were isolated from unfixed cryo-sections of kidneys and livers of rats exposed to ...
M Stoltenberg, S Juhl, G Danscher
doaj   +1 more source

Chemical analysis of soluble fractions from normal and autolysed rabbit liver by column chromatography [PDF]

open access: yes, 1966
Chromatography on Sephadex G-200 was performed with the soluble fraction of homogenated rabbit liver, which was extracted with 0.1 M phosphate buffer containing 0.1 M NaCl.
Aizawa, Tadashi   +4 more
core   +1 more source

Separation of dog serum lipoproteins by ultracentrifugation, dextran sulfate precipitation, and paper electrophoresis*

open access: yesJournal of Lipid Research, 1961
The separation of dog serum lipoproteins by ultracentrifugation at a density of 1.063 is hindered by the failure of the high density fraction to accumulate in the bottom portion of the centrifuge tube.
Toshio Sakagami, D.B. Zilversmit
doaj   +1 more source

Construction of a novel phagemid to produce custom DNA origami scaffolds. [PDF]

open access: yes, 2018
DNA origami, a method for constructing nanoscale objects, relies on a long single strand of DNA to act as the 'scaffold' to template assembly of numerous short DNA oligonucleotide 'staples'.
Aksel, Tural   +2 more
core   +1 more source

Subcellular Fractionation of Cultured Human Cell Lines

open access: yesBio-Protocol, 2013
Subcellular localization is crucial for the proper functioning of a protein. Deregulation of subcellular localization may lead to pathological consequences and result in diseases like cancer.
Zhuoyou Yu, Zhiguang Huang, Maria Lung
doaj   +1 more source

Elucidation of the mechanism of subunit exchange in αB crystallin oligomers

open access: yesScientific Reports, 2021
AlphaB crystallin (αB-crystallin) is a key protein for maintaining the long-term transparency of the eye lens. In the eye lens, αB-crystallin is a “dynamical” oligomer regulated by subunit exchange between the oligomers.
Rintaro Inoue   +10 more
doaj   +1 more source

Optimization Protocol of the PEG-Based Method for OSCC-Derived Exosome Isolation and Downstream Applications

open access: yesSeparations, 2022
The exosome precipitation method affects the purity of the exosome and the quality of the downstream application. Polymer-based precipitation is a cost-effective method widely used in different research fields.
Tzong-Ming Shieh   +5 more
doaj   +1 more source

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