Results 51 to 60 of about 70,242 (336)

Isolation of Extracellular Vesicles Derived from Mesenchymal Stromal Cells by Ultracentrifugation

Bio-Protocol, 2020
Extracellular vesicles (EVs) are a heterogeneous group of membranous vesicles that differ on their biogenesis and release pathways, such as exosomes, microvesicles and apoptotic bodies.
María José Ramírez-Bajo, Elisenda Banon-Maneus, Jordi Rovira, Josep M Campistol, Fritz Diekmann   +4 more
doaj   +1 more source

Isolation, characterisation and experimental evolution of phage that infect the horse chestnut tree pathogen, Pseudomonas syringae pv. aesculi [PDF]

, 2020
Bleeding canker of horse chestnut trees is a bacterial disease, caused by the bacterium Pseudomonas syringae pv. aesculi, estimated to be present in ~ 50% of UK horse chestnut trees.
Jackson, Robert W., James, Sarah L., Neuman, Benjamin W., Percival, Glynn, Rabiey, Mojgan   +4 more
core   +3 more sources

Autophagosome marker, LC3, is released extracellularly via several distinct pathways

FEBS Open Bio, EarlyView.
This study establishes a novel HiBiT‐tagging system for ultrasensitive detection of LC3, revealing multiple pathways for its extracellular secretion. It demonstrates that LC3 is released via both autophagy‐dependent and ‐independent mechanisms, including a novel route for nonlipidated LC3‐I.
Koki Saito, Masashi Arakawa, Koki Maeda, Eiji Morita   +3 more
wiley   +1 more source

Practical Aspects of Multiwavelength Analytical Ultracentrifugation

Instruments, 2019
Open-source Multiwavelength Analytical Ultracentrifugation (MWL-AUC) detection systems have been evolving for over a decade. Continual advances emerging out of several research groups have brought the instrumentation technology to increasingly higher ...
Joseph Pearson, Helmut Cölfen
doaj   +1 more source

RNA‐Micelles as Self‐Assembling Structures for Efficient Co‐Delivery of Synergistic siRNA and Nucleoside Analogues to Treat CRC Lung Metastasis

Advanced Functional Materials, EarlyView.
Two kinds of self‐assembled RNA micelles were used to co‐deliver synergistic siRNA and nucleoside analogues for the treatment of colorectal cancer lung metastases. Near‐complete elimination of lung cancer metastases was confirmed in an orthotopic lung metastasis model constructed using human colorectal cancer lung metastases patient surgical samples to
Kai Jin, Piotr Rychahou, Daniel W. Binzel, B. Mark Evers, Peixuan Guo   +4 more
wiley   +1 more source

A novel three step protocol to isolate extracellular vesicles from plasma or cell culture medium with both high yield and purity

Journal of Extracellular Vesicles, 2020
Extracellular vesicles (EV) are membrane encapsulated nanoparticles that can function in intercellular communication, and their presence in biofluids can be indicative for (patho)physiological conditions.
Xiaogang Zhang, Ellen G. F. Borg, A. Manuel Liaci, Harmjan R. Vos, Willem Stoorvogel   +4 more
doaj   +1 more source

Alternative Method for HDL and Exosome Isolation with Small Serum Volumes and Their Characterizations

Separations, 2021
High-density lipoprotein (HDL) and exosomes are promising sources of biomarkers. However, the limited sample volume and access to the ultracentrifuge equipment are still an issue during HDL and exosome isolation.
Rian Ka Praja, Wisitsak Phoksawat, Patcharaporn Tippayawat, Amonrat Jumnainsong, Chanvit Leelayuwat   +4 more
doaj   +1 more source

Cytoplasmic DNA in the unfertilized sea urchin egg: Physical properties of circular mitochondrial DNA and the occurrence of catenated forms [PDF]

, 1968
The mitochondrial DNA in the unfertilized egg of the sea urchin Lytechinus pictus is present in an amount approximately seven times that of the haploid nuclear DNA.(1) The mitochondrial DNA has a higher buoyant density than the nuclear DNA and consists ...
Blair, Donald G., Pikó, Lajos, Tyler, Albert, Vinograd, Jerome   +3 more
core  

High‐Resolution Characterization of Protein‐Conjugated, mRNA‐Loaded Lipid Nanoparticles by Analytical Ultracentrifugation

Advanced Functional Materials, EarlyView.
Lipid nanoparticles containing messenger RNA are characterized by sedimentation velocity analytical ultracentrifugation using UltraScan's new Custom Grid algorithm to provide multi‐dimensional distributions for partial specific volume (particle density), molar mass, sedimentation, diffusion, and hydrodynamic radius.
Sophia Bird, Connor Smith, Nahal Habibi, Samantha Rivera, Saeed Mortezazadeh, Reece Martin, Benjamin M. Geilich, Gilles Besin, Borries Demeler   +8 more
wiley   +1 more source

Particle size and protein content of six fractions of the Sf > 20 plasma lipoproteins isolated by density gradient centrifugation

Journal of Lipid Research, 1969
A procedure is described for the separation of plasma Sf > 400 and Sf 20-400 lipoproteins each into three fractions. Serum samples are overlayered with a sodium chloride density gradient in a preparative ultracentrifuge tube and thin layers are removed ...
W.J. Lossow, F.T. Lindgren, J.C. Murchio, G.R. Stevens, L.C. Jensen   +4 more
doaj   +1 more source