Results 31 to 40 of about 51,747 (268)

Symposium on Ultrafast Electron Microscopy and Ultrafast Science [PDF]

Microscopy and Microanalysis, 2009
Dynamic characterization techniques have been utilized in the fields of biology, chemistry, physics, and materials science for many years. Techniques range from neutron scattering to X-ray diffraction. Two of the fields experiencing much development recently have been electron-based techniques. Namely, ultrafast electron diffraction (UED) and ultrafast
Mitra L. Taheri, Nigel D. Browning, John Lewellen   +2 more
openaire   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Compact laser-driven plasma X-ray source for time-resolved diffraction, spectroscopy and imaging experiments at ELI Beamlines

Journal of Synchrotron Radiation
In this work, experimentally measured characteristics of a kilohertz laser-driven Cu plasma X-ray source that was recently commissioned at the ELI Beamlines facility are reported. The source can be driven either by an in-house developed high-contrast sub-
Y. Pulnova, T. Parkman, B. Angelov, I. Baranova, A. Zymaková, S. Cipiccia, L. Fardin, B. A. Yorke, R. Antipenkov, D. Peceli, O. Hort, D.-D. Mai, J. Andreasson, J. Nejdl   +13 more
doaj   +1 more source

Toward time-resolved laser T-jump/X-ray probe spectroscopy in aqueous solutions

Structural Dynamics, 2019
Most chemical and biochemical reactions in nature and in industrial processes are driven by thermal effects that bring the reactants above the energy barrier for reaction.
O. Cannelli, C. Bacellar, R. A. Ingle, R. Bohinc, D. Kinschel, B. Bauer, D. S. Ferreira, D. Grolimund, G. F. Mancini, M. Chergui   +9 more
doaj   +1 more source

Energy Scaling of Infrared Femtosecond Pulses by Dual-Chirped Optical Parametric Amplification

IEEE Photonics Journal, 2017
We present an energy-scaling experiment on a femtosecond infrared pulse using dual-chirped optical parametric amplification (DC-OPA). A total output energy exceeding 100 mJ with 36-fs pulse duration was achieved in the infrared region, which is ...
Yuxi Fu, Eiji J. Takahashi, Katsumi Midorikawa   +2 more
doaj   +1 more source

Molecular dynamics simulations of positively selected codons in FcγRI reveal novel biochemical binding properties

FEBS Open Bio, EarlyView.
Evolutionary analysis across 32 placental mammals identified positive selection at residues H148 and W149 in the immune receptor FcγR1. Ancestral reconstruction combined with molecular dynamics simulations reveals how these mutations may influence receptor structure and dynamics, providing insight into the evolution of antibody recognition and immune ...
David A. Young, Omar Arias‐Gaguancela, Fiona Gibson, Morgan Grainger, Jodie Score, Amit Nathubhai, Mauricio Cafiero, Lee Richard Machado   +7 more
wiley   +1 more source

Assessing Different PCR Master Mixes for Ultrarapid DNA Amplification: Important Analytical Parameters

Diagnostics
The basic principles of ultrafast plasmonic PCR have been promulgated in the scientific and technological literature for over a decade. Yet, its everyday diagnostic utility remains unvalidated in pre-clinical and clinical settings.
Ivan Brukner, Miltiadis Paliouras, Mark Trifiro, Marc Bohbot, Daniel Shamir, Andrew G. Kirk   +5 more
doaj   +1 more source

Optimizing photoexcitation conditions for time‐resolved X‐ray solution scattering experiments

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering (TR‐XSS) is a powerful technique to visualize how proteins change their structure in real time after light activation. Selecting the right laser photoexcitation conditions—fluence, excitation geometry, and sample refresh rate—is critical to maximize the experimental signal while avoiding unwanted side effects ...
Matteo Levantino
wiley   +1 more source

Ultrafast Coherent Spectroscopy [PDF]

, 1988
Coherent spectroscopy, initially started in the mid-sixties, is intimately conncected with the development of intense coherent light sources. The generation of ultrashort light pulses enabled coherent spectroscopy to be extended to real-time measurements of rapid dynamic processes on the time scale of 10−12 s.
Zinth, Wolfgang, Kaiser, Wolfgang
openaire   +2 more sources

Uniform Amorphization of Crystalline Silicon Surfaces Enabled by Deep Ultraviolet Femtosecond Laser Pulses

Advanced Engineering Materials, EarlyView.
Deep‐UV (258 nm) femtosecond pulses enable uniform amorphous silicon writing on Si(100)/(111) with a six‐fold larger fluence amorphization window than NIR methods. Optimized fluence and overlap yield 20–45 nm uniform and continuous amorphous layers. Microscopy shows sharp interfaces, and real‐time reflectivity reveals nanosecond melt–resolidification ...
Wissal Benali, Magali Gregoire, Matys Guyard, Tatiana Itina, David Grojo, Pol Sopeña   +5 more
wiley   +1 more source