Results 121 to 130 of about 625 (152)

Efficient production of ulvan lyase from Ulva prolifera by Catenovulum sp. LP based on stage-controlled fermentation strategy

Algal Research, 2020
Abstract Ulvan lyase, specifically depolymerizing ulvan to unsaturated sulfated oligosaccharides with diverse biological activities, is becoming more and more popular. To provide an economical fermentative strategy, Catenovulum sp. LP cultured in low-cost medium that directly used Ulva prolifera as the main carbon source was used to produce ulvan ...
Leke Qiao, Xiaoke Yang, Ruize Xie, Chunying Du, Yongzhou Chi, Jingliang Zhang, Peng Wang   +6 more
openaire   +1 more source

New Ulvan-Degrading Polysaccharide Lyase Family: Structure and Catalytic Mechanism Suggests Convergent Evolution of Active Site Architecture

ACS Chemical Biology, 2017
Ulvan is a complex sulfated polysaccharide biosynthesized by green seaweed and contains predominantly rhamnose, xylose, and uronic acid sugars. Ulvan-degrading enzymes have only recently been identified and added to the CAZy ( www.cazy.org ) database as family PL24, but neither their structure nor catalytic mechanism(s) are yet known.
Ulaganathan, Thirumalaiselvi, Boniecki, Michal, Foran, Elizabeth, Buravenkov, Vitaliy, Mizrachi, Naama, Banin, Ehud, Helbert, William, Cygler, Miroslaw   +7 more
openaire   +3 more sources

Improving the thermostability of ulvan lyase from polysaccharide lyase family 25 based on multiple computational rational design strategies

International Journal of Biological Macromolecules
A rational design strategy to improve the thermal stability of enzymes is essential for advancing industrial applications. This study proposes a computer-aided rational design strategy combining deep learning and multiple energy function methods to enhance the thermal stability of ulvan lyase. ColabFold was employed for structure prediction.
Chunying, Du, Huining, Li, Man, Zhang, Xinyu, Wang, Laigui, Xing, Lei, Wan, Ximing, Xu, Peng, Wang   +7 more
openaire   +2 more sources

Biochemical Characterization of a Novel Thermostable Ulvan Lyase from Tamlana fucoidanivorans CW2-9

Journal of Agricultural and Food Chemistry
Ulvan is a complex sulfated polysaccharide extracted from Ulva, and ulvan lyases can degrade ulvan through a β-elimination mechanism to obtain oligosaccharides. In this study, a new ulvan lyase, EPL15085, which belongs to the polysaccharide lyase (PL) 28 family from Tamlana fucoidanivorans CW2-9, was characterized in detail. The optimal pH and salinity
Yan, Xu, Jin, Li, Lu, An, Yuankai, Qiu, Aihua, Mao, Zhixiao, He, Jialing, Guo, Hanbing, Yan, Han, Li, Zhong, Hu   +9 more
openaire   +2 more sources

Fine chemical structure analysis of oligosaccharides produced by an ulvan-lyase degradation of the water-soluble cell-wall polysaccharides from Ulva sp. (Ulvales, Chlorophyta)

Carbohydrate Research, 1997
A marine bacterium degrading the water-soluble cell wall polysaccharides from Ulva sp. (ulvan) has been isolated. The good correlation between ulvan degradation monitored by reducing-power, UV absorbance and viscosimetry, indicated that the crude enzymatic extract contains essentially an endo-ulvan lyase activity. This activity was rapidly inhibited by
Lahaye, Marc, Brunel, M., Bonnin, Estelle   +2 more
openaire   +3 more sources

Strategies for Improving the Protein Yield in pH-Shift Processing of Ulva lactuca Linnaeus: Effects of Ulvan Lyases, pH-Exposure Time, and Temperature

ACS Sustainable Chemistry & Engineering, 2019
Globally, there is a need for novel vegetarian protein sources. We recently showed that the pH-shift process, using alkaline protein solubilization followed by isoelectric precipitation, is an efficient way to produce extracts with high protein concentrations from Ulva lactuca (>50% on a dry matter basis).
Hanna Harrysson, Venkat Rao Konasani, Gunilla B. Toth, Henrik Pavia, Eva Albers, Ingrid Undeland   +5 more
openaire   +1 more source

NMR spectroscopic characterisation of oligosaccharides from two Ulva rigida ulvan samples (Ulvales, Chlorophyta) degraded by a lyase

Carbohydrate Research, 1998
The chemical structure and the sequence of repeating units in ulvans of similar compositions from two different Ulva rigida samples collected in the Canary Islands and in Brittany were studied after ulvan-lyase degradation and NMR spectroscopic analysis of the reaction products.
openaire   +3 more sources

Improving the thermal stability and catalytic activity of ulvan lyase by the combination of FoldX and KnowVolution campaign

International Journal of Biological Macromolecules
Thermal stability is one of the most important properties of ulvan lyases for their application in algae biomass degradation. The Knowledge gaining directed eVolution (KnowVolution) protein engineering strategy could be employed to improve thermostability of ulvan lyase with less screening effort.
Ailan, Huang, Zhengqi, Chen, Xinming, Wu, Wenxing, Yan, Fuping, Lu, Fufeng, Liu   +5 more
openaire   +2 more sources

PT215 Detection of Alginate Lyase and Ulvan Lyase Activity in Tropically Isolated Biodegraders

2021
Mothafar Sadek, Sveltana Kotelnikova, Nikisha Bartholomew, Romany Nightingale   +3 more
openaire   +1 more source

Efficient preparation and production of ulvan oligosaccharides by using a new PL25 family ulvan lyase from Alteromonas sp. 76–1

Journal of Applied Phycology, 2023
Tiancheng Tang, Chen Li, Benwei Zhu, Zhong Yao   +3 more
openaire   +1 more source