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Biochemical Characterization of a Novel Thermostable Ulvan Lyase from Tamlana fucoidanivorans CW2-9

Journal of Agricultural and Food Chemistry
Ulvan is a complex sulfated polysaccharide extracted from Ulva, and ulvan lyases can degrade ulvan through a β-elimination mechanism to obtain oligosaccharides. In this study, a new ulvan lyase, EPL15085, which belongs to the polysaccharide lyase (PL) 28 family from Tamlana fucoidanivorans CW2-9, was characterized in detail. The optimal pH and salinity
Yan, Xu   +9 more
openaire   +2 more sources

Strategies for Improving the Protein Yield in pH-Shift Processing of Ulva lactuca Linnaeus: Effects of Ulvan Lyases, pH-Exposure Time, and Temperature

ACS Sustainable Chemistry & Engineering, 2019
Globally, there is a need for novel vegetarian protein sources. We recently showed that the pH-shift process, using alkaline protein solubilization followed by isoelectric precipitation, is an efficient way to produce extracts with high protein concentrations from Ulva lactuca (>50% on a dry matter basis).
Hanna Harrysson   +5 more
openaire   +1 more source

NMR spectroscopic characterisation of oligosaccharides from two Ulva rigida ulvan samples (Ulvales, Chlorophyta) degraded by a lyase

Carbohydrate Research, 1998
The chemical structure and the sequence of repeating units in ulvans of similar compositions from two different Ulva rigida samples collected in the Canary Islands and in Brittany were studied after ulvan-lyase degradation and NMR spectroscopic analysis of the reaction products.
openaire   +3 more sources

Improving the thermal stability and catalytic activity of ulvan lyase by the combination of FoldX and KnowVolution campaign

International Journal of Biological Macromolecules
Thermal stability is one of the most important properties of ulvan lyases for their application in algae biomass degradation. The Knowledge gaining directed eVolution (KnowVolution) protein engineering strategy could be employed to improve thermostability of ulvan lyase with less screening effort.
Ailan, Huang   +5 more
openaire   +2 more sources

PT215 Detection of Alginate Lyase and Ulvan Lyase Activity in Tropically Isolated Biodegraders

2021
Mothafar Sadek   +3 more
openaire   +1 more source

Creation of the Ulvan Lyase Enzyme Complex for the Production of Ulvan-Derived Oligosaccharides

Journal of Agricultural and Food Chemistry
YeWon Jo   +5 more
openaire   +1 more source

Ulvan Lyase

Peng Wang   +3 more
openaire   +1 more source

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