Results 1 to 10 of about 77,033 (163)
Structural and mechanistic classification of uronic acid-containing polysaccharide lyases [PDF]
Glycobiology, 2010 Polysaccharide lyases (PLs) have been assigned to 21 families based on their sequences, with ~ 50 singletons awaiting further classification. For 19 of these families, the structure of at least one protein is known. In this review, we have analyzed the available structural information and show that presently known PL families belong to six general ...
Garron, Marie-Line, Cygler, Miroslaw
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Functional Exploration of the Polysaccharide Lyase Family PL6 [PDF]
PLOS ONE, 2016 Alginate, the main cell-wall polysaccharide of brown algae, is composed of two residues: mannuronic acid (M-residues) and, its C5-epimer, guluronic acid (G-residues). Alginate lyases define a class of enzymes that cleave the glycosidic bond of alginate by β-elimination.
Mathieu, Sophie +4 more
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Probing the pH Effects on Sugar Binding to a Polysaccharide Lyase [PDF]
The Journal of Physical Chemistry B, 2019 Polysaccharide lyases (PLs) are an important class of proteins that are excreted from bacteria to degrade sugars in the extracellular matrix of the host. The PL from S. maltophilia (Smlt1473) was found to have pH-specific degradation of three varying polysaccharides: alginate, celluronic acid, and hyaluronic acid (J. Biol. Chem. 2014, 289, 18022-18032).
Sook Wong +3 more
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vAL‐1, a novel polysaccharide lyase encoded by chlorovirus CVK2 [PDF]
FEBS Letters, 2004 Cell wall materials isolated from Chlorella cells were degraded by the polysaccharide‐degrading enzyme vAL‐1 encoded by chlorovirus CVK2. Matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometric analyses of the degradation products (oligosaccharides) revealed major oligosaccharides contain unsaturated GlcA at the reducing terminus,
Sugimoto, Ichiro +4 more
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ACS Central Science, 2022 Dynamics is an essential process to drive an enzyme to perform a function. When a protein sequence encodes for its three-dimensional structure and hence its function, it essentially defines the intrinsic dynamics of the molecule. The static X-ray crystal structure was thought to shed little insight into the molecule's dynamics until the recently ...
Prerana Dash, Rudresh Acharya
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Structure and Polymannuronate Specificity of a Eukaryotic Member of Polysaccharide Lyase Family 14 [PDF]
Journal of Biological Chemistry, 2017 Alginate is an abundant algal polysaccharide, composed of β-d-mannuronate and its C5 epimer α-l-guluronate, that is a useful biomaterial in cell biology and tissue engineering, with applications in cancer and aging research. The alginate lyase (EC 4.2.2.3) from Aplysia kurodai, AkAly30, is a eukaryotic member of the polysaccharide lyase 14 (PL-14 ...
Hui-Min, Qin +8 more
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Engineering broad-spectrum digestion of polyuronides from an exolytic polysaccharide lyase [PDF]
Biotechnology for Biofuels, 2016 Macroalgae represents a promising source of fermentable carbohydrates for use in the production of energy efficient biofuel. The primary carbohydrate in brown algae is the uronic acid-containing alginate, whereas green algae contains a significant amount of glucuronan.
MacDonald, Logan C. +2 more
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Advanced Science, EarlyView.AM symbiosis induces GhWAK13 to promote fungal colonization. GhNTF3 negatively regulates symbiosis by promoting SA biosynthesis. GhWAK13 sequesters GhNTF3 at the membrane, limiting its nuclear translocation and relieving SA‐mediated suppression. Under Verticillium stress, GhNTF3 enters the nucleus and interacts with GhJAZ6 to activate SA signaling ...
Shuangjie Jia +10 more
wiley +1 more source
A Metagenome‐Assembled Genome Catalog From the Global Ruminant Microbiomes
Animal Research and One Health, EarlyView.The Ruminant Gastrointestinal MAG Catalog (RGMC) is a comprehensive global resource offering 40,812 strain‐level genomes across 53 bacterial and 4 archaeal classes. It greatly surpasses prior efforts in scale and diversity, serving as an essential foundation for research in ruminant nutrition, microbial function, and methane mitigation.
Shizhe Zhang +8 more
wiley +1 more source
Crystal Structure of Family 14 Polysaccharide Lyase with pH-dependent Modes of Action [PDF]
Journal of Biological Chemistry, 2009 The Chlorella virus enzyme vAL-1 (38 kDa), a member of polysaccharide lyase family 14, degrades the Chlorella cell wall by cleaving the glycoside bond of the glucuronate residue (GlcA) through a beta-elimination reaction. The enzyme consists of an N-terminal cell wall-attaching domain (11 kDa) and a C-terminal catalytic module (27 kDa).
Kohei, Ogura +5 more
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