Results 11 to 20 of about 6,770 (172)
An evolutionarily distinct family of polysaccharide lyases removes rhamnose capping of complex arabinogalactan proteins. [PDF]
J Biol Chem, 2017 The human gut microbiota utilizes complex carbohydrates as major nutrients. The requirement for efficient glycan degrading systems exerts a major selection pressure on this microbial community. Thus, we propose that this microbial ecosystem represents a substantial resource for discovering novel carbohydrate active enzymes.
Munoz-Munoz J +5 more
europepmc +6 more sources
Probing the pH Effects on Sugar Binding to a Polysaccharide Lyase [PDF]
The Journal of Physical Chemistry B, 2019 Polysaccharide lyases (PLs) are an important class of proteins that are excreted from bacteria to degrade sugars in the extracellular matrix of the host. The PL from S. maltophilia (Smlt1473) was found to have pH-specific degradation of three varying polysaccharides: alginate, celluronic acid, and hyaluronic acid (J. Biol. Chem. 2014, 289, 18022-18032).
Sook Wong +3 more
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Marine Drugs, 2022 Fucoidans represent a type of polyanionic fucose-containing sulfated polysaccharides (FCSPs) that are cleaved by fucoidan-degrading enzymes, producing low-molecular-weight fucoidans with multiple biological activities suitable for pharmacological use ...
Juanjuan Wang +7 more
doaj +1 more source
Phylogenetic Distribution of Polysaccharide-Degrading Enzymes in Marine Bacteria
Frontiers in Microbiology, 2021 Deconstruction is an essential step of conversion of polysaccharides, and polysaccharide-degrading enzymes play a key role in this process. Although there is recent progress in the identification of these enzymes, the diversity and phylogenetic ...
Zhong-Zhi Sun +12 more
doaj +1 more source
Genome-Wide Analysis of PL7 Alginate Lyases in the Genus Zobellia
Molecules, 2021 We carried out a detailed investigation of PL7 alginate lyases across the Zobellia genus. The main findings were obtained using the methods of comparative genomics and spatial structure modeling, as well as a phylogenomic approach. Initially, in order to
Nadezhda Chernysheva +4 more
doaj +1 more source
vAL‐1, a novel polysaccharide lyase encoded by chlorovirus CVK2 [PDF]
FEBS Letters, 2004 Cell wall materials isolated from Chlorella cells were degraded by the polysaccharide‐degrading enzyme vAL‐1 encoded by chlorovirus CVK2. Matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometric analyses of the degradation products (oligosaccharides) revealed major oligosaccharides contain unsaturated GlcA at the reducing terminus,
Sugimoto, Ichiro +4 more
openaire +2 more sources
Biotechnology for Biofuels, 2020 Background As the most abundant polysaccharide in brown algae, alginate has become a promising economical material for bioethanol production. Recently, exo-type alginate lyases have received extensive attention because the unsaturated monosaccharides ...
Luyao Tang +6 more
doaj +1 more source
Structural and mechanistic classification of uronic acid-containing polysaccharide lyases [PDF]
Glycobiology, 2010 Polysaccharide lyases (PLs) have been assigned to 21 families based on their sequences, with ~ 50 singletons awaiting further classification. For 19 of these families, the structure of at least one protein is known. In this review, we have analyzed the available structural information and show that presently known PL families belong to six general ...
Garron, Marie-Line, Cygler, Miroslaw
openaire +2 more sources
Molecular Plant-Microbe Interactions, 2006 Two pectate lyase genes (Bx-pel-1 and Bx-pel-2) were cloned from the pine wood nematode, Bursaphelenchus xy-lophilus. The deduced amino acid sequences of these pectate lyases are most similar to polysaccharide lyase family 3 proteins.
Taisei Kikuchi +3 more
doaj +1 more source
Untangling the Reaction Mechanism of the Polysaccharide Lyase PL42 Using QM/MM Metadynamics Simulations. [PDF]
J Chem Inf ModelQM/MM metadynamics elucidates the β-elimination mechanism of the PL42 L-rhamnose-α-1,4-D-glucuronate lyase FoRham1. The GlcA +1 sugar follows a 1 C4 → 1 C4 → 1 H2 itinerary and reacts via a concerted, highly asynchronous syn β-elimination with a carbanion-like transition state. H85 acts as the catalytic base within a triad with D83 and H105.
Alonso-Gil S +3 more
europepmc +2 more sources