Results 81 to 90 of about 316 (111)

Creation of the Ulvan Lyase Enzyme Complex for the Production of Ulvan-Derived Oligosaccharides

Journal of Agricultural and Food Chemistry
Ulvan, a primary constituent of green algae biomass, has the potential to be used for various biomedical and biotechnology applications. Its biological activity is significantly influenced by its molecular weight. However, the high molecular weight of ulvan poses challenges due to its high viscosity and poor solubility, necessitating an efficient ...
Jihyun Lee, Jeongeun Hyeon, Sung Ok Han   +2 more
exaly   +3 more sources

Ulvan lyase from Formosa agariphila and its applicability in depolymerisation of ulvan extracted from three different Ulva species

Algal Research, 2018
Members of green macroalgae cause green tides in eutrophicated coastal waters. These green tides pose an environmental issue and an economic burden on coastal municipalities. The biomass from these green tides has a potential to be used as feedstock in biorefinery due to the content of interesting biomacromolecules.
Chunsheng Jin, Eva Albers, Niclas G Karlsson   +2 more
exaly   +3 more sources

Efficient preparation and production of ulvan oligosaccharides by using a new PL25 family ulvan lyase from Alteromonas sp. 76–1

Journal of Applied Phycology, 2023
Benwei Zhu, Yao Zhong, Zhu Benwei
exaly   +2 more sources

Ulvan polysaccharide-degrading enzymes: An updated and comprehensive review of sources category, property, structure, and applications of ulvan lyases

Algal Research, 2021
Abstract Ulvan, extracted from the marine green algae belonging to the genus Ulva, is mainly composed of four monosaccharides consisting of rhamnose, xylose, glucuronic acid and iduronic acid. It has great potential in the fields of food, pharmaceuticals and chemistry.
Tiancheng Tang, Shengsheng Cao, Benwei Zhu, Qian Li   +3 more
openaire   +3 more sources

Biochemical characterization of an ulvan lyase from the marine flavobacterium Formosa agariphila KMM 3901T

Applied Microbiology and Biotechnology, 2018
Carbohydrates are the product of carbon dioxide fixation by algae in the ocean. Their polysaccharides are depolymerized by marine bacteria, with a vast array of carbohydrate-active enzymes. These enzymes are important tools to establish biotechnological processes based on algal biomass.
Lukas Reisky, Christian Stanetty, Marko D. Mihovilovic, Thomas Schweder, Jan-Hendrik Hehemann, Uwe T. Bornscheuer   +5 more
openaire   +3 more sources

Biochemical characteristics and high-efficiency expression of a novel PL24 family ulvan lyase

International Journal of Biological Macromolecules
Ulvan lyase can degrade ulvan into ulva oligosaccharides with higher water solubility and better biological activity. This study characterized the enzymatic properties of a novel ulvan lyase ALT559 and enhanced its expression level. ALT559 was composed of 530 amino acid residues and exhibited the highest identity to ulvan lyases of the PL24 family.
Laigui, Xing, Chunying, Du, Wenqin, Chu, Man, Zhang, Lei, Wan, Fang, He, Peng, Wang   +6 more
openaire   +2 more sources

Mechanistic insights into catalysis of a novel polysaccharide lyase family 40 ulvan lyase from Thalassomonas sp. LD5

International Journal of Biological Macromolecules
As one of the primary structural components in green algal cell walls, ulvan can be enzymatically degraded by ulvan lyase to produce oligosaccharides with diverse biological activities. In this study, TsUly40A, a new PL40 family ulvan lyase from Thalassomonas sp.
Zheng, Fu, Wenjun, Wang, Siyi, Lv, Chengying, Yin, Xiaofei, Wang, Xiaohui, Sun, Runying, Zeng, Fei, Xu, Wengong, Yu, Feng, Han   +9 more
openaire   +2 more sources

Improving the thermal stability and catalytic activity of ulvan lyase by the combination of FoldX and KnowVolution campaign

International Journal of Biological Macromolecules
Thermal stability is one of the most important properties of ulvan lyases for their application in algae biomass degradation. The Knowledge gaining directed eVolution (KnowVolution) protein engineering strategy could be employed to improve thermostability of ulvan lyase with less screening effort.
Ailan, Huang, Zhengqi, Chen, Xinming, Wu, Wenxing, Yan, Fuping, Lu, Fufeng, Liu   +5 more
openaire   +2 more sources

Biochemical characterization and immobilization of a PL25 family ulvan lyase for efficient degradation of Enteromorpha polysaccharide

International Journal of Biological Macromolecules
Green algal polysaccharides such as ulvan and Enteromorpha polysaccharides (EPs) with versatile physiological activities have drawn increasing attentions as a renewable biomass resource. However, their activities and applications have been greatly limited by its high molecular weight and low solubility.
Penghui, Zhu, Bochen, Pan, Chen, Li, Benwei, Zhu, Zhong, Yao, Yun, Sun   +5 more
openaire   +2 more sources

New Ulvan-Degrading Polysaccharide Lyase Family: Structure and Catalytic Mechanism Suggests Convergent Evolution of Active Site Architecture

ACS Chemical Biology, 2017
Ulvan is a complex sulfated polysaccharide biosynthesized by green seaweed and contains predominantly rhamnose, xylose, and uronic acid sugars. Ulvan-degrading enzymes have only recently been identified and added to the CAZy ( www.cazy.org ) database as family PL24, but neither their structure nor catalytic mechanism(s) are yet known.
Ulaganathan, Thirumalaiselvi, Boniecki, Michal, Foran, Elizabeth, Buravenkov, Vitaliy, Mizrachi, Naama, Banin, Ehud, Helbert, William, Cygler, Miroslaw   +7 more
openaire   +3 more sources