Results 151 to 160 of about 99,447 (295)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Venous reconstruction in living donor liver transplantation: lessons learned from a new national program in a resource-limited setting. [PDF]

Transpl Int
Semash K, Dzhanbekov T, Nasirov M, Subanov A, Umarov B.   +4 more
europepmc   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Unification Studies and Scenario Planning

, 2009
Unification studies are a kind of interdisciplinary studies sui generis with regard to the realization of Korean unification. They are not static, but dynamic in the sense that their main focus and combination and hierarchy of major subjects have been
박형중
core  

Towards theoretical foundation for high-precision fringe projection profilometry system design and optimization. [PDF]

Light Sci Appl
Zhang S.
europepmc   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Transcatheter Electrosurgical Unification of a Fenestrated Atrial Septal Defect for Single Device Closure. [PDF]

JACC Case Rep
Lyons-Ewing DA, McAlister C, Puri A, Troughton RW, Adamson PD.   +4 more
europepmc   +1 more source

Refinement of amino‐acid conformation vs. difference density maps in time‐resolved serial femtosecond crystallography data analysis

FEBS Open Bio, EarlyView.
The dFoCC pipeline starts with observed DED and resting‐state coordinates, which are then used to generate a library of triggered states. Correlation analysis of the calculated DED features of each candidate vs observed DED permits quantitative evaluation of candidate structural quality.
Meng Iao Fong, Yuhei Hosokawa, Lars Oliver Essen, Manuel Maestre‐Reyna   +3 more
wiley   +1 more source

The Relationship between Democracy Inclination and Unification Perception: A Recommendation to the Unification Policy

, 2009
In these days South Korea has faced some difficulties in pursuing the unification policy effectively. The civil society groups divided into left and right to the political orientation.
김병조
core  

Toward a unified data-driven turbulence model through multi-objective learning. [PDF]

Natl Sci Rev
Liu Z, Wang H, Zhao Z, Xiao H.
europepmc   +1 more source