Neuropragmatics: from classical pragmatics to neurocognitive models of pragmatics in dialogue. [PDF]
Gutiérrez Cisneros A +2 more
europepmc +1 more source
Versatile vector tools for efficient protein screening across multiple expression systems
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu +5 more
wiley +1 more source
Bonding Without Bridging: Social Capital, Integration, and Well-Being Among Filipina Marriage Migrants in South Korea. [PDF]
Miranda AT, Miranda JLF, Kim E.
europepmc +1 more source
UiO‐66(Zr) metal–organic frameworks are chemically stable, biocompatible, and highly tunable nanomaterials. Their modular structure enables controlled drug delivery, multimodal bioimaging, and light‐activated photodynamic therapy, supporting integrated diagnostic and therapeutic (theranostic) applications in cancer and biomedical research.
Veronika Huntošová +2 more
wiley +1 more source
Synergistic Integration of Enzyme and Microbial Platforms for Sustainable Management of Pharmaceutical Pollutants: Towards a Greener Pharmaceutical Lifecycle. [PDF]
Sun Z, Chen P, Ge X, Zhang W, Liu H.
europepmc +1 more source
The crystal structure of Borrelia burgdorferi nicotinamidase (PncA/BBE22) reveals the correct full‐length protein initiated from a non‐canonical AUU start codon. The structure validates previous biochemical findings and resolves a long‐standing annotation error, demonstrating that the truncated database sequence is structurally incompatible with the ...
Kalvis Brangulis
wiley +1 more source
Mechanically Activated Transition from Linear Viscoelasticity to Yielding: Correlation-Based Unification. [PDF]
Arzhakov MS +3 more
europepmc +1 more source
Directed evolution of enzymes at the crossroads of tradition and innovation
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova +2 more
wiley +1 more source
Identification of resistance sources and genomic regions regulating spot blotch resistance in Asian bread wheat (Triticum aestivum L.) via genome-wide association study. [PDF]
Aggarwal N +6 more
europepmc +1 more source
Guiding AlphaFold to predict how Munc13‐1 opens Syntaxin‐1
The syntaxin‐1 Habc‐domain (orange), linker (pink) and SNARE motif (yellow) form a closed conformation that binds to Munc18‐1 (violet) and is opened by the Munc13‐1 MUN domain (cyan) to form the SNARE complex that triggers neurotransmitter release.
Madhurima Chattopadhyay +2 more
wiley +1 more source

