Results 111 to 120 of about 38,300 (288)

Versatile vector tools for efficient protein screening across multiple expression systems

open access: yesFEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu   +5 more
wiley   +1 more source

Batch uniformity and energy efficiency improvements on a cabinet dryer suitable for smallholder farmers

open access: yes, 2015
Drying can add value, facilitate transport and extend the storage life of agro-products; therefore, in developing countries with poorly established cool-chains, drying is particularly effective.
Mahayothee, Busarakorn   +3 more
core   +1 more source

Cyclic azapeptide CD36 ligand attenuates cardiac injury and reduces long‐chain fatty acid accumulation after myocardial ischemia–reperfusion in mice

open access: yesFEBS Open Bio, EarlyView.
In a murine model of myocardial ischemia and reperfusion (MI/R), the CD36 azapeptide ligand MPE‐298 reduces cardiac injury and transiently lowers left ventricular long‐chain fatty acids (LCFAs) accumulation 3 h after reperfusion, accompanied by a decrease of oxidative stress and inflammation‐associated genes' expression in the heart and adipose tissue.
Jade Gauvin   +12 more
wiley   +1 more source

Relationships between agronomic traits and characterization of the white oat ideotype for cultivation with and without chemical fertilization

open access: yesRevista Thema
This paper aimed to characterize and verify whether the linear relationships between agronomic traits of white oat are different between crops with and without chemical fertilization; and identify the agronomic ideotype that enhances the agronomic ...
Murilo Vieira Loro   +6 more
doaj   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

open access: yesFEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova   +2 more
wiley   +1 more source

Transcripts enriched in codons that trigger P‐site tRNA‐mediated mRNA decay possess stable mRNA

open access: yesFEBS Open Bio, EarlyView.
PTMD codons were first described by Mendel et al. as mediators of an mRNA decay pathway dependent on the human protein CNOT3, homologous to yeast Not5. Our findings confirm that PTMD codons destabilize transcripts; however, unlike in yeast, the human pathway specifically targets and slightly destabilizes primarily stable mRNAs.
Rodolfo Lopes Carneiro   +1 more
wiley   +1 more source

Rethinking Patent Law’s Uniformity Principle

open access: yes, 2007
Modern law on expert testimony insists, as a condition of admissibility, that the asserted expertise be determined by the trial judge to be reliable. Reliability is usually characterized as a dichotomous attribute of evidence, as if expertise were either
Nard, Craig Allen, Duffy, John F.
core  

How phagocytic cells kill bacteria: Lessons from a professional killer

open access: yesFEBS Open Bio, EarlyView.
How phagocytic cells ingest and kill bacteria has been studied for more than a century, but many questions remain unanswered. The study of the amoeba Dictyostelium discoideum brings new answers, and new questions. Professional phagocytic cells such as neutrophils and macrophages, as well as free‐living soil amoebae like Dictyostelium discoideum, employ
Otmane Lamrabet, Pierre Cosson
wiley   +1 more source

Assessment of layer thickness and uniformity in railway embankments with Ground Penetrating Radar [PDF]

open access: yes, 2008
In the aim of a national research project entitled “Interaction soil-rail track for high speed trains”, a protocol was established between the National Railway Network and four national research institutions to develop the knowledge concerning the ...
Caldeira, Laura   +4 more
core  

Effect of dietary protein content on growth, uniformity and mortality of two commercial broiler strains

open access: yes, 2009
Two experiments were conducted to determine the response in performance, including uniformity and mortality, of two broiler strains to dietary protein content.
Gous, RM, Behre, ET
core   +1 more source

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