Results 81 to 90 of about 1,145 (256)

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

open access: yesFEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz   +6 more
wiley   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

Chameleon sequences reveal structural effects in proteins representing micelle‐like distribution of hydrophobicity

open access: yesFEBS Open Bio, EarlyView.
Amino acids sequence of two different proteins with the same sequence (chameleon sequence—black boxes) represent in 3D structure of the proteins different secondary structures: HHHH—helical and BBB—Beta‐structural. The chains folded in water environment adopt different III‐order structures in which the chameleon fragments appear to adopt similar status
Irena Roterman   +4 more
wiley   +1 more source

Problemi lineari e non lineari di flusso intorno ad ostacoli

open access: yesLe Matematiche, 2005
We discuss the problem of the steady two-dimensional flow past fixed disturbances in an open channel of finite depth. We consider different types of obstacles, like submerged or surface-piercing bodies, and localized perturbations of a horizontal bottom;
Dario Pierotti
doaj  

Corrigendum to “Solvability and uniqueness criteria for generalized Sylvester-type equations”

open access: yesLinear Algebra and its Applications, 2018
zbMATH Open Web Interface contents unavailable due to conflicting licenses.
De Terán, Fernando   +3 more
openaire   +4 more sources

Development of human monoclonal antibodies against TARM1 by yeast display

open access: yesFEBS Open Bio, EarlyView.
Human monoclonal antibodies against TARM1 are generated by yeast display‐guided selection. These antibodies bind to soluble and cell‐surface forms of TARM1. Also, these antibodies exhibit agonistic activity in the NFAT‐GFP reporter assay, indicating that TARM1 signaling can be functionally modulated by antibodies and suggesting TARM1 as a potential ...
Rikio Yabe   +5 more
wiley   +1 more source

Evolution Equations with Liouville Derivative on R without Initial Conditions

open access: yesMathematics
New classes of evolution differential equations with the Liouville derivative in Banach spaces are studied. Equations are considered on the whole real line and are not endowed by the initial conditions.
Vladimir E. Fedorov, Nadezhda M. Skripka
doaj   +1 more source

Matrix metalloproteinase‐9 regulates cell adhesion and membrane protrusive activity of ovarian cancer cells

open access: yesFEBS Open Bio, EarlyView.
Matrix metalloproteinase‐9 (MMP9) drives ovarian cancer progression. Using MMP9‐null cells (M9‐KO) created from ovarian cancer cells, we found MMP9 loss did not block Epidermal Growth Factor (EGF)‐driven E‐cadherin dissolution or EMT but delayed and reduced EGF‐driven membrane protrusions. Transient MMP9 re‐expression drove membrane protrusion.
Claire Strauel   +8 more
wiley   +1 more source

On the Unique Solvability of a Nonlinear Reaction-Diffusion Model with Convection

open access: yesJournal of Mathematical Analysis and Applications, 2001
The main goal of this article is to establish the uniqueness and comparison results for the nonnegative solution to the Cauchy-Dirichlet problem \[ u= u_0\quad\text{on}\quad\overline{\Omega}\times\{0\}; \quad u =\psi \quad \text{on}\quad \partial\Omega \] for the reaction-diffusion-convection equation \[ u_t = \Delta\varphi (x, t, u) + \nabla \cdot G(x,
openaire   +1 more source

Decreased cold‐sensing function of the transient receptor potential channel TRPM8 from tailed amphibians

open access: yesFEBS Open Bio, EarlyView.
Despite frogs avoiding low temperatures, examination of four salamander species revealed that none avoided cold and all possessed cold tolerance. Functional analysis of TRPM8, a cold sensor, showed that all salamander TRPM8s had lost their cold sensitivity.
Tadahiro Sawao   +3 more
wiley   +1 more source

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