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Regulation of transcription by unnatural amino acids
Nature Biotechnology, 2011Small-molecule regulation of gene expression is intrinsic to cellular function and indispensable to the construction of new biological sensing, control and expression systems. However, there are currently only a handful of strategies for engineering such regulatory components and fewer still that can give rise to an arbitrarily large set of inducible ...
Chang C, Liu +3 more
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Probing lysine posttranslational modifications by unnatural amino acids
Chemical Communications, 2022Application of structurally and chemically diverse unnatural amino acids in biomolecular studies of lysine posttranslational modifications is described in this Feature Article.
Marijn N. Maas +2 more
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Protein Design Using Unnatural Amino Acids
Journal of Chemical Education, 2003With the increasing availability of whole organism genome sequences, understanding protein structure and function is of capital importance. Recent developments in the methodology of incorporation of unnatural amino acids into proteins allow the exploration of proteins at a very detailed level. Furthermore, de novo design of novel protein structures and
Basar Bilgiçer, Krishna Kumar
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The unnatural culture of amino acids
Nature Methods, 2007Researchers describe a general and highly efficient approach for incorporating a variety of unnatural amino acids into proteins at desired locations in mammalian cells.
Corinne D Hausmann +2 more
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Activity of yeast d-amino acid oxidase on aromatic unnatural amino acids
Journal of Molecular Catalysis B: Enzymatic, 2008D-Amino acid oxidase is a FAD-dependent enzyme that catalyses the conversion of the D-enantiomer of amino acids into the corresponding a-keto acid. Substrate specificity of the enzyme from the yeast Rhodotorula gracilis was investigated towards aromatic amino acids, and particularly synthetic a-amino acids.
Caligiuri, A. +7 more
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Introduction of unnatural amino acids into chalcone isomerase
Bioconjugate Chemistry, 1991The active site cysteine residue of chalcone isomerase was rapidly and selectively modified under denaturing conditions with a variety of electrophilic reagents. These denatured and modified enzyme were renatured to produce enzyme derivatives containing a series of unnatural amino acids in the active site.
R A, Bednar, C, McCaffrey, K, Shan
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Enzymatic Conversion of Unnatural Amino Acids by Yeast
AbstractUnnatural amino acids, particularly synthetic α‐amino acids, are becoming crucial tools for modern drug discovery research. In particular, this application requires enantiomerically pure isomers. In this work we report on the resolution of racemic mixtures of the amino acids d,l‐naphthylalanine and d,l‐naphthylglycine by using a natural enzyme,
CALIGIURI A +6 more
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A Phage Display System with Unnatural Amino Acids
Journal of the American Chemical Society, 2004We report a general method to display peptide-containing unnatural amino acids on filamentous M13 phage. Five distinct unnatural amino acids were site-specifically incorporated at the N-terminal of the M13 phage minor coat protein pIII. Phages that contain p-azidophenylalanine can undergo a highly specific azide-alkyne [3 + 2] cycloaddition reaction ...
Feng, Tian +2 more
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Incorporation of unnatural amino acids for synthetic biology
Mol. BioSyst., 2010The challenge of synthetic biology lies in the construction of artificial cellular systems. This requires the development of modular "parts" that can be integrated into living systems to elicit an artificial, yet programmed, response or function. The development of methods to engineer proteins bearing unnatural amino acids (UAAs) provides essential ...
Natalya, Voloshchuk, Jin Kim, Montclare
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Unnatural Protein Engineering: Producing Proteins with Unnatural Amino Acids
Medicinal Chemistry Reviews - Online, 2005Less than a decade ago, the ability to generate proteins with unnatural modifications was a Herculean task available only to specialty labs. Recent advances make it possible to generate reasonable quantities of protein with un- natural amino acids both in vitro and in vivo .
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