Results 311 to 320 of about 165,169 (375)

Elucidating the genetic mechanisms governing cytosine base editing outcomes through CRISPRi screens. [PDF]

Nat Commun
Gu S, Bodai Z, Anderson RA, So HYA, Cowan QT, Komor AC.   +5 more
europepmc   +1 more source

Organic Transistor‐Based Biosensors for Viral RNA Detection and Variant Monitoring: From SARS‐CoV‐2 to Effective Management of Future Pandemics

Advanced Sensor Research, Volume 4, Issue 6, June 2025.
A novel approach for direct sensing of viral RNA is proposed, allowing PCR‐free detection of viral RNA from genomic material, and variants screening by discrimination of polymorphic amplicons. The device is a cost‐effective, user‐friendly platform suitable for both mass screening kits and as a laboratory tool for an optimized management of resources ...
Stefano Lai, Roberto Cusano, Alessandra Scano, Giorgio Fotia, Annalisa Bonfiglio   +4 more
wiley   +1 more source

Synthesis of pyrrolo[3,2-<i>d</i>]pyrimidine-2,4(3<i>H</i>)-diones by domino C-N coupling/hydroamination reactions. [PDF]

Beilstein J Org Chem
Figueira de Abreu RM, Tiedemann R, Ehlers P, Langer P.   +3 more
europepmc   +1 more source

Assessment and Mitigation of CRISPR‐Cas9‐Induced Nontargeted Translocations

Advanced Science, Volume 12, Issue 21, June 5, 2025.
Various inverted repeat elements are dispersed throughout genomes. This study reveals that these elements can cause significant chromosomal rearrangements when CRISPR editing occurs in their proximity. The risk can be mitigated by incorporating inverted repeat‐homologous segments into the CRISPR system, which represents a promising strategy for ...
Zhiyang Hou, Qiyi Yi, Mengying Wu, Lijun Wu, Fanghua Li, Ting Wang, Po Bian   +6 more
wiley   +1 more source

Targeting PTBP3‐Mediated Alternative Splicing of COX11 Induces Cuproptosis for Inhibiting Gastric Cancer Peritoneal Metastasis

Advanced Science, Volume 12, Issue 21, June 5, 2025.
The splicing factor PTBP3 promotes COX11 exon skipping, allowing gastric cancer organoids to evade cuproptosis. Antisense oligonucleotide drugs targeting PTBP3‐mediated COX11 alternative splicing, in combination with copper ionophores, promote cuproptosis in organoids, thereby providing a therapeutic approach for gastric cancer peritoneal metastasis ...
Yajing Zhou, Chao Dong, Xiaochun Shen, Pengbo Wang, Tao Chen, Weikang Li, Xiaotong Sun, Peiyuan Li, Chengxiang Xu, Kaipeng Duan, Dongbao Li, Jin Zhou   +11 more
wiley   +1 more source

Targeted deaminase-free T-to-G and C-to-K base editing in rice by fused human uracil DNA glycosylase variants. [PDF]

Plant Biotechnol J
Wu Y, Wang X, Wang H, Han Y, Wang Y, Zou C, Zhu JK, Li M.   +7 more
europepmc   +1 more source

Recent Advances in Xenes Based FET for Biosensing Applications

Advanced Science, Volume 12, Issue 21, June 5, 2025.
Xenes (monoelemental 2D materials like graphene, phosphorene, tellurene) enable ultrasensitive FET (Field‐Effect Transistor) biosensors due to their tunable bandgap, high carrier mobility, and large surface area. Advances in synthesis (CVD, MBE) and functionalization allow detection of DNA/RNA (via CRISPR‐Cas systems), proteins, small molecules, and ...
Huide Wang, Chen Wang, Yule Zhang, Ziqian Wang, Yihan Zhu, Yun Wang, Xiangqian Hong, Han Zhang, Ning Fan, Meng Qiu   +9 more
wiley   +1 more source

Spraying of water microdroplets forms luminescence and causes chemical reactions in surrounding gas. [PDF]

Sci Adv
Meng Y, Xia Y, Xu J, Zare RN.
europepmc   +1 more source

Advances in CRISPR‐Cas9 in lineage tracing of model animals

Animal Models and Experimental Medicine, Volume 8, Issue 6, Page 1004-1022, June 2025.
Cell lineage tracing is a key technology for describing the developmental history of individual progenitor cells and assembling them to form a lineage development tree. However, traditional methods have limitations of poor stability and insufficient resolution.
Jingchao Cao, Zihang Guo, Xueling Xu, Pan Li, Yi Fang, Shoulong Deng   +5 more
wiley   +1 more source

Engineering Saccharomyces Cerevisiae With Novel Functional Xylose Isomerases From Rumen Microbiota for Enhanced Biofuel Production

Biotechnology Journal, Volume 20, Issue 6, June 2025.
Graphical Abstract and Lay Summary This study identified novel xylose isomerases (XIs) from ruminal microbiota using omics data. Five Bacillota XIs were efficiently expressed in Saccharomyces cerevisiae, enabling effective conversion of xylose into xylulose and subsequent ethanol production.
Beatriz de Oliveira Vargas, Marcelo Falsarella Carazzolle, Juliana Pimentel Galhardo, Juliana José, Brenda Cristina de Souza, Jéssica Batista de Lima Correia, Jade Ribeiro dos Santos, Gonçalo Amarante Guimarães Pereira, Fellipe da Silveira Bezerra de de Mello   +8 more
wiley   +1 more source