Results 311 to 320 of about 165,169 (375)
Elucidating the genetic mechanisms governing cytosine base editing outcomes through CRISPRi screens. [PDF]
Gu S+5 more
europepmc +1 more source
A novel approach for direct sensing of viral RNA is proposed, allowing PCR‐free detection of viral RNA from genomic material, and variants screening by discrimination of polymorphic amplicons. The device is a cost‐effective, user‐friendly platform suitable for both mass screening kits and as a laboratory tool for an optimized management of resources ...
Stefano Lai+4 more
wiley +1 more source
Synthesis of pyrrolo[3,2-<i>d</i>]pyrimidine-2,4(3<i>H</i>)-diones by domino C-N coupling/hydroamination reactions. [PDF]
Figueira de Abreu RM+3 more
europepmc +1 more source
Assessment and Mitigation of CRISPR‐Cas9‐Induced Nontargeted Translocations
Various inverted repeat elements are dispersed throughout genomes. This study reveals that these elements can cause significant chromosomal rearrangements when CRISPR editing occurs in their proximity. The risk can be mitigated by incorporating inverted repeat‐homologous segments into the CRISPR system, which represents a promising strategy for ...
Zhiyang Hou+6 more
wiley +1 more source
The splicing factor PTBP3 promotes COX11 exon skipping, allowing gastric cancer organoids to evade cuproptosis. Antisense oligonucleotide drugs targeting PTBP3‐mediated COX11 alternative splicing, in combination with copper ionophores, promote cuproptosis in organoids, thereby providing a therapeutic approach for gastric cancer peritoneal metastasis ...
Yajing Zhou+11 more
wiley +1 more source
Targeted deaminase-free T-to-G and C-to-K base editing in rice by fused human uracil DNA glycosylase variants. [PDF]
Wu Y+7 more
europepmc +1 more source
Recent Advances in Xenes Based FET for Biosensing Applications
Xenes (monoelemental 2D materials like graphene, phosphorene, tellurene) enable ultrasensitive FET (Field‐Effect Transistor) biosensors due to their tunable bandgap, high carrier mobility, and large surface area. Advances in synthesis (CVD, MBE) and functionalization allow detection of DNA/RNA (via CRISPR‐Cas systems), proteins, small molecules, and ...
Huide Wang+9 more
wiley +1 more source
Spraying of water microdroplets forms luminescence and causes chemical reactions in surrounding gas. [PDF]
Meng Y, Xia Y, Xu J, Zare RN.
europepmc +1 more source
Advances in CRISPR‐Cas9 in lineage tracing of model animals
Cell lineage tracing is a key technology for describing the developmental history of individual progenitor cells and assembling them to form a lineage development tree. However, traditional methods have limitations of poor stability and insufficient resolution.
Jingchao Cao+5 more
wiley +1 more source
Graphical Abstract and Lay Summary This study identified novel xylose isomerases (XIs) from ruminal microbiota using omics data. Five Bacillota XIs were efficiently expressed in Saccharomyces cerevisiae, enabling effective conversion of xylose into xylulose and subsequent ethanol production.
Beatriz de Oliveira Vargas+8 more
wiley +1 more source