Results 241 to 250 of about 61,889 (286)
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Immobilization of cholinesterase and urease
Analytical Biochemistry, 1970Abstract Procedures are described for the immobilization (insolubilization) of the enzymes cholinesterase and urease in starch and polyacrylamide. Various parameters affecting the immobilization are discussed, and the stability of the insolubilized enzymes in storage and in use is reported.
G G, Guilbault, J, Das
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Journal of the American Chemical Society, 2005
At the active site of urease, urea undergoes nucleophilic attack by water, whereas urea decomposes in solution by elimination of ammonia so that its rate of spontaneous hydrolysis is unknown. Quantum mechanical simulations have been interpreted as indicating that urea hydrolysis is extremely slow, compared with other biological reactions proceeding ...
Brian P, Callahan +2 more
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At the active site of urease, urea undergoes nucleophilic attack by water, whereas urea decomposes in solution by elimination of ammonia so that its rate of spontaneous hydrolysis is unknown. Quantum mechanical simulations have been interpreted as indicating that urea hydrolysis is extremely slow, compared with other biological reactions proceeding ...
Brian P, Callahan +2 more
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Soybean leaf urease: Comparison with seed urease
Physiologia Plantarum, 1983Soybeans, Glycine max (L.) Merr., from ureides for transport of nitrogen from the root nodule to the shoot. The most direct routes for ureide utilization include the degradation of ureide‐derived urea to NH3 and CO2. Ureolytic activity was found in leaf disks of soybean and exhbited optimal activity at pH 7 in the presence of a high concentration of ...
P. S. Kerr +3 more
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Incorporation of urease into liposomes
Biochimica et Biophysica Acta (BBA) - General Subjects, 1977Abstract 1. 1. Urease was entrapped in egg lecithin liposomes. About 1 mg of protein was incorporated per 10 mg of lipid, which corresponds to a molar lipid: protein ration of about 4600. 2. 2. The entrapment procedure led to a change of the apparent Michaelis constant from about 68 mM (free enzyme) to about 167 mM (entrapped enzyme). The V
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American Journal of Physiology-Legacy Content, 1961
Ammonium intoxication in dogs was induced by intravenous administration of urease. This method affords a unique preparation for the study of ammonia metabolism in vivo, since, within limits, a self-perpetuating, cyclic release of ammonia is achieved.
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Ammonium intoxication in dogs was induced by intravenous administration of urease. This method affords a unique preparation for the study of ammonia metabolism in vivo, since, within limits, a self-perpetuating, cyclic release of ammonia is achieved.
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Inhibition of urease by hydroxyurea
Biochemical Pharmacology, 1965Abstract Hydroxyurea was shown to be a potent inhibitor of urease; 50% inhibition occurred at a concentration of approximately 5 × 10−5 M. Preincubation of the enzyme with the inhibitor prior to addition of substrate yielded inhibition which progressed with time, whereas simultaneous addition of inhibitor and substrate to the enzyme reduced the ...
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Analytical Biochemistry, 1970
Abstract An important sensing electrode has been developed for assay of the enzyme urease. It is based on the peak or steady-state potential obtained with the aid of a roller pump to control the concentration of urea against the surface of an ammonium ion electrode.
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Abstract An important sensing electrode has been developed for assay of the enzyme urease. It is based on the peak or steady-state potential obtained with the aid of a roller pump to control the concentration of urea against the surface of an ammonium ion electrode.
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