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Urease is a nickel-containing enzyme encoded by several microorganisms and plants since its physiological role, involving the conversion of urea into ammonia, is crucial for their survival and/or pathogenicity. The story of urease is rich in Guinness records, i.e., being the first enzyme crystallized, a milestone in the understanding of the protein ...
D'Agostino I., Carradori S.
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D'Agostino I., Carradori S.
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Immobilization of cholinesterase and urease
Analytical Biochemistry, 1970Abstract Procedures are described for the immobilization (insolubilization) of the enzymes cholinesterase and urease in starch and polyacrylamide. Various parameters affecting the immobilization are discussed, and the stability of the insolubilized enzymes in storage and in use is reported.
J. Das, George G. Guilbault
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Incorporation of urease into liposomes
Biochimica et Biophysica Acta (BBA) - General Subjects, 1977Abstract 1. 1. Urease was entrapped in egg lecithin liposomes. About 1 mg of protein was incorporated per 10 mg of lipid, which corresponds to a molar lipid: protein ration of about 4600. 2. 2. The entrapment procedure led to a change of the apparent Michaelis constant from about 68 mM (free enzyme) to about 167 mM (entrapped enzyme). The V
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American Journal of Physiology-Legacy Content, 1961
Ammonium intoxication in dogs was induced by intravenous administration of urease. This method affords a unique preparation for the study of ammonia metabolism in vivo, since, within limits, a self-perpetuating, cyclic release of ammonia is achieved.
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Ammonium intoxication in dogs was induced by intravenous administration of urease. This method affords a unique preparation for the study of ammonia metabolism in vivo, since, within limits, a self-perpetuating, cyclic release of ammonia is achieved.
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Journal of the American Chemical Society, 2005
At the active site of urease, urea undergoes nucleophilic attack by water, whereas urea decomposes in solution by elimination of ammonia so that its rate of spontaneous hydrolysis is unknown. Quantum mechanical simulations have been interpreted as indicating that urea hydrolysis is extremely slow, compared with other biological reactions proceeding ...
Brian P. Callahan +2 more
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At the active site of urease, urea undergoes nucleophilic attack by water, whereas urea decomposes in solution by elimination of ammonia so that its rate of spontaneous hydrolysis is unknown. Quantum mechanical simulations have been interpreted as indicating that urea hydrolysis is extremely slow, compared with other biological reactions proceeding ...
Brian P. Callahan +2 more
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Quantitative studies on the inhibition of crystalline urease by rabbit anti-urease
Archives of Biochemistry and Biophysics, 1955Abstract 1. 1. Quantitative precipitin studies with crystalline urease and antiurease have been performed. The resulting data provide information concerning the combining proportions of the enzyme and antibody on a weight basis. 2. 2. Urease is inhibited by rabbit anti-urease. 3. 3.
Manfred M. Mayer, A. Alvin Marucci
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Quantification of Urease Activity
2019Urease is one of the most distinctive virulence factors of Proteus mirabilis pathogenesis. Urease activity correlates with many landmark side effects of P. mirabilis catheter-associated urinary tract infections, such as urolithiasis and bacteremia.
Shawn Richmond, Alejandra Yep
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Urease Metabolism in Citrullus
Nature, 1951SINCE the speculations by one of us1 as to the function of urease in Citrullus seeds appear to have aroused interest, we feel we ought to report that further work has shown the original hypothesis (that urease is a reserve protein) to be untenable. We remain unconvinced that the metabolism of urease in Citrullus is connected with the metabolism of urea
P. Ch. Sharma, W. T. Williams
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Canadian Journal of Biochemistry, 1970
Ureolytically active urease with a sedimentation coefficient of 12 S has been observed as a major component following centrifugation in sucrose gradients prepared with citrate buffers of high ionic strengths.
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Ureolytically active urease with a sedimentation coefficient of 12 S has been observed as a major component following centrifugation in sucrose gradients prepared with citrate buffers of high ionic strengths.
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Urease biogenesis in Streptococcus thermophilus
Research in Microbiology, 2005Urease biogenesis was monitored in the lactic acid bacterium Streptococcus thermophilus during the growth cycle using in-gel detection and a phenol-hypochloride assay. Zymogram analysis, performed in a non-denaturing polyacrylamide gel, enabled visualization of a complex profile of bands whose number and intensity were dependent on the growth phase and
Mora D. +8 more
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