Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
FEBS Open Bio, EarlyView.Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source
Evolución de pacientes operados de tumor de hipofisis en el servicio de neurocirugía del Hospital escuela Antonio Lenin Fonseca [PDF]
, 2016 Se realizó este estudio de Cohortes prospectivas en el servicio de Neurocirugía del Hospital Escuela Antonio Lenin Fonseca en el período comprendido del 9 de Febrero del 2015 al 12 de Enero del 2016 que trata sobre la evolución de 26 pacientes operados ...
González, Arlen, Hernández, Rolando
core
FEBS Open Bio, EarlyView.Abruptly changing from aerobic to anaerobic conditions (sudden anaerobization) induced growth inhibition and a significant increase in intracellular labile ferrous iron in the aerotolerant anaerobe Amphibacillus xylanus. We found that free flavins mediate efficient electron transfer from NADH to ferric iron under anaerobic conditions, suggesting that ...
Shinya Kimata +13 more
wiley +1 more source
Diagnóstico para el mejoramiento de la vía terciaria que comunica la vereda Puente Piedra y el municipio de Madrid, Cundinamarca [PDF]
, 2019 Trabajo de InvestigaciónEl proyecto determinó el diagnóstico para el mejoramiento de la vía que comunica la vereda Puente Piedra con el municipio de Madrid, Cundinamarca, con lo cual se realizó visitas de campo, estudios de suelos para caracterización ...
Carrión-Rincón, Camilo Alberto +1 more
core
RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells
FEBS Open Bio, EarlyView.RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour +2 more
wiley +1 more source
Evaluation of liver tumor identification rate of volumetric-interpolated breath-hold images using the compressed sensing method and qualitative evaluation of tumor contrast effect via visual evaluation. [PDF]
Quant Imaging Med Surg, 2022 Yoshimaru D +8 more
europepmc +1 more source
FEBS Open Bio, EarlyView.UiO‐66(Zr) metal–organic frameworks are chemically stable, biocompatible, and highly tunable nanomaterials. Their modular structure enables controlled drug delivery, multimodal bioimaging, and light‐activated photodynamic therapy, supporting integrated diagnostic and therapeutic (theranostic) applications in cancer and biomedical research.
Veronika Huntošová +2 more
wiley +1 more source
Directed evolution of enzymes at the crossroads of tradition and innovation
FEBS Open Bio, EarlyView.An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova +2 more
wiley +1 more source
Face shape processing via visual-to-auditory sensory substitution activates regions within the face processing networks in the absence of visual experience. [PDF]
Front Neurosci, 2022 Arbel R, Heimler B, Amedi A.
europepmc +1 more source
Annals of Clinical and Translational Neurology, EarlyView.ABSTRACT Objective Super‐Refractory Status Epilepticus (SRSE) is a rare, life‐threatening neurological emergency with unclear etiology in many cases. Mitochondrial dysfunction, often due to disease‐causing genetic variants, is increasingly recognized as a cause, with each gene producing distinct pathophysiological mechanisms.
Pouria Mohammadi +2 more
wiley +1 more source