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2002
Publisher Summary This chapter describes methods for studying different aspects of vacuole biogenesis and function. It presents three different approaches for visualizing the yeast vacuole: staining with vital dyes, using green fluorescent protein (GFP)-tagged vacuolar marker proteins, and immunofluorescence microscopy of formaldehyde-fixed cells ...
Elizabeth, Conibear, Tom H, Stevens
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Publisher Summary This chapter describes methods for studying different aspects of vacuole biogenesis and function. It presents three different approaches for visualizing the yeast vacuole: staining with vital dyes, using green fluorescent protein (GFP)-tagged vacuolar marker proteins, and immunofluorescence microscopy of formaldehyde-fixed cells ...
Elizabeth, Conibear, Tom H, Stevens
openaire +2 more sources
Journal of Experimental Biology, 1992
ABSTRACT Plant cells are unique in containing large acidic vacuoles which occupy most of the cell volume. The vacuolar H+-ATPase (V-ATPase) is the enzyme responsible for acidifying the central vacuole, although it is also present on Golgi and coated vesicles.
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ABSTRACT Plant cells are unique in containing large acidic vacuoles which occupy most of the cell volume. The vacuolar H+-ATPase (V-ATPase) is the enzyme responsible for acidifying the central vacuole, although it is also present on Golgi and coated vesicles.
openaire +2 more sources