A Variational Multiscale method with immersed boundary conditions for incompressible flows [PDF]
Soonpil Kang, Arif Masud
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Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková +3 more
wiley +1 more source
Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke +4 more
wiley +1 more source
R-function method and variational method for the bending problem of functionally graded plates with fixed supports and complex shapes. [PDF]
Su K, Li S.
europepmc +1 more source
Analyzing doubly heavy tetra- and penta-quark states by variational method
Ruilin Zhu +3 more
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A Variational EM Method for Pole-Zero Modeling of Speech with Mixed Block Sparse and Gaussian Excitation [PDF]
Liming Shi +3 more
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Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane +11 more
wiley +1 more source
Intercompartmental communication in senescence
Senescent cells experience structural changes in the plasma membrane, endoplasmic reticulum, mitochondria, lysosomes, nucleus, and cytoskeleton. These alterations disrupt crosstalk among cellular compartments, impairing vesicular trafficking, contact sites, and molecular flow.
Krystyna Mazan‐Mamczarz +3 more
wiley +1 more source
Variational Method for Vibration Analysis of Elliptic Cylinders Reinforced with Functionally Graded Carbon Nanotubes. [PDF]
Gong Q, Liu T, Teng Y, Ma B, Li X.
europepmc +1 more source

