Biology, Bionomics and Molecular Biology of Anopheles sinensis Wiedemann 1828 (Diptera: Culicidae), Main Malaria Vector in China [PDF]
Frontiers in Microbiology, 2017 China has set a goal to eliminate all malaria in the country by 2020, but it is unclear if current understanding of malaria vectors and transmission is sufficient to achieve this objective.
Xinyu Feng +8 more
semanticscholar +5 more sources
Gene amplification by PCR and subcloning into a GFP‐fusion plasmid expression vector as a molecular biology laboratory course* [PDF]
Biochemistry and Molecular Biology Education, 2004 AbstractA novel experimental sequence for the advanced undergraduate laboratory course has been developed at Earlham College. Utilizing recent improvements in molecular techniques for a time‐sensitive environment, undergraduates were able to create a chimera of a selected gene and green fluorescent protein (GFP) in a bacterial expression plasmid over ...
Joshua A. Bornhorst +2 more
semanticscholar +5 more sources
An updated pGREEN-based vector suite for cost-effective cloning in plant molecular biology
microPublication biology, 2020 Figure 1: A) Basic map of pG20-toolkit plasmids. B-F) Proof of concept for C-terminal fusion proteins of the pG20 toolkit using transient expression in Nicotiana benthamiana leaves. B) Empty YFP and C) empty RFP localized in the cytosol or nucleus. D) As
Anna I Pratt +2 more
semanticscholar +5 more sources
Editorial: Special Issue on the “Molecular Biology of Disease Vectors” [PDF]
International Journal of Molecular Sciences, 2023 Arthropod disease vectors not only transmit malaria but many other serious diseases, many of which are, to a greater or lesser degree, neglected [...]
Michail Kotsyfakis
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Retrovirus vectors and their uses in molecular biology [PDF]
BioEssays, 1986 AbstractRetroviral vectors utilize the biochemical processes unique to retroviruses, to transfer genes with high efficiency into a wide variety of cell types in tissue culture and in living animals. With such vectors, the effect of newly introduced genes and the mechanism of gene expression can be studied in cell types so far refractory to other ...
Eli Gilboa
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Seamless, modular binary vectors for plant cell and molecular biology [PDF]
Plant Biotechnology JournalSummaryIn plants, genetic manipulations are commonly performed using Agrobacterium tumefaciens, a plant pathogen whose abilities as a ‘natural genetic engineer’ have been co‐opted for biotechnological applications. In the widely used binary vector systems, Agrobacterium already contains a plasmid with most of the genes needed for virulence and is ...
R M Greenhalgh +5 more
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Tạp chí Truyền nhiễm Việt Nam, 2021 Molecular biology techniques are increasingly being used in scientific research and supply for medicine to contribute response the duty about taking care and protecting people’s health. Institute of Malariology - Parasitology - Entomology in Ho Chi Minh City (IMPE HCM) has deployed the application about some molecular biology techniques to detect ...
Nguyen Thuy Vy Pham +4 more
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Comparison of the standard WHO susceptibility tests and the CDC bottle bioassay for the determination of insecticide susceptibility in malaria vectors and their correlation with biochemical and molecular biology assays in Benin, West Africa [PDF]
Parasites & Vectors, 2013 The detection of insecticide resistance in natural populations of Anopheles vectors is absolutely necessary for malaria control. In the African region, the WHO insecticide susceptibility test is the most common method for assessing resistance status.
Nazaire Aïzoun +8 more
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, 2016 Emerging infectious diseases (EIDs) continue to threaten human and animal welfare worldwide. Vector-borne pathogens are particularly concerning because of their ubiquity, pathogenicity, and lack of predictably due to insufficient ecological and evolutionary knowledge regarding vector species.
Matthew W. Hopken
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Spatial distribution and insecticide resistance of Aedes mosquitoes in Osun State: implications for vector control [PDF]
Tropical Medicine and HealthBackground Aedes mosquitoes are primary vectors of arboviral diseases, such as dengue, chikungunya, and Zika, posing major threats to tropical public health. Understanding their spatial distribution and resistance status is vital for sustainable control.
L. O. Busari +16 more
doaj +2 more sources