Results 161 to 170 of about 600,320 (348)

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

EMBO Conference on Molecular and Population Biology of Mosquitoes and Other Disease Vectors: From Basic Vector Biology to Disease Control

Pathogens and Global Health, 2014
George, Phillip, Jensen, Silke, Vaury, Chantal, Sharakhov, Igor   +3 more
openaire   +3 more sources

Molecular study of geminiviruses: Complex biology, host-vector interactions, and increasing diversity

Journal of King Saud University - Science, 2022
Muhammad Arif, Shahid Farooq, Abdulrahman Alasmari, Mohammed Ali Alshehri, Mohamed Hashem, Saad Alamri, Hassan A. Hemeg, Nadiyah M. Alabdallah   +7 more
openaire   +1 more source

Promiscuous stimulation of HSP70 ATPase activity by parasite‐derived J‐domains

FEBS Open Bio, EarlyView.
The malaria parasite Plasmodium falciparum exports three highly homologous yet functionally divergent J‐domain proteins into human erythrocytes. Here, we show that J‐domains isolated from all three proteins effectively stimulate the ATPase activity of both endogenous host and exported parasite HSP70 chaperones.
Julian Barth, Moritz Koch, Le‐Han Rössner, Johanna Eichhorn, Denys Pogoryelov, Matthias P. Mayer, Jude M. Przyborski   +6 more
wiley   +1 more source

Discovery of an Adaptive Neuroimmune Response Driving Itch and Fast Tick Removal with Implications for Preventing Pathogen Transmission

Advanced Science
Acquired tick resistance (ATR) is well characterized in tick‐exposed animals, compromising tick fitness through antibody‐mediated activation of basophils. Yet, anti‐tick vaccines inducing ATR have had limited success.
Johannes S. P. Doehl, Tiago D. Serafim, Serena Doh, Charles S. Grugan, Eva Iniguez, Luana Rogerio, Ronja Frigard, Ranadhir Dey, Pedro Cecilio, Xinglong Gu, Pang‐Yen Tseng, Aline da Silva Moreira, Mahnaz Minai, James Oristian, Hans Ackerman, Steven Brooks, Caroline Percopo, Siu‐Ping Ng, Derron A. Alves, Lucas Tirloni, Jennifer M. Anderson, Adriana Marques, Fabiano Oliveira, Shaden Kamhawi, Daniel E. Sonenshine, José M. C. Ribeiro, Mark Hoon, Jesus G. Valenzuela   +27 more
doaj   +1 more source

An Integrative Phylogenetic Analysis of the Genus Rhynchium Spinola (Hymenoptera: Vespidae: Eumeninae) from China Based on Morphology, Genomic Data and Geographical Distribution

Insects
The species of the genus Rhynchium Spinola are important natural insect control resources and widespread in the Old World. Due to the various color patterns of several species in China, it is difficulty to accurately classify and identify the genus.
Yun-Lian Peng, Shu-Lin He, Bin Chen, Ting-Jing Li   +3 more
doaj   +1 more source

Development of human monoclonal antibodies against TARM1 by yeast display

FEBS Open Bio, EarlyView.
Human monoclonal antibodies against TARM1 are generated by yeast display‐guided selection. These antibodies bind to soluble and cell‐surface forms of TARM1. Also, these antibodies exhibit agonistic activity in the NFAT‐GFP reporter assay, indicating that TARM1 signaling can be functionally modulated by antibodies and suggesting TARM1 as a potential ...
Rikio Yabe, Mayumi Saeki, Masaaki Hashiguchi, Sayaka Ono, Kazuhisa Aoki, Hidetaka Tanno   +5 more
wiley   +1 more source

OVEREXPRESSION, PURIFICATION AND CHARACTERIZATION OF INOSITOL 1,4,5-TRISPHOSPHATE 3-KINASE FROM RAT-BRAIN

, 2018
The entire gene encoding D-myo-inositol 1,4,5-trisphosphate 3-kinase (IP3K) of rat brain was overexpressed using a T7 promoter in an expression vector pT7-7. When induced by isopropyl-beta-D-thiogalactoside, E.
SHIN, YS, HOI, KY, CHOI, G
core  

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Cloning, purification and characterization of a thermostable beta-galactosidase from Bacillus licheniformis strain KG9

, 2015
A thermo- and alkalitolerant Bacillus licheniformis KG9 isolated from Taslidere hot water spring in Batman/Turkey was found to produce a thermostable ?-galactosidase. Phylogenetic analysis showed that the 16S rRNA gene from B.
Bekler, Fatma Matpan, Acer, Ömer, Güven, Reyhan Gül, Guven, K., Acer, O., Guven, R. Gul, Stougaard, Peter, Güven, Kemal, Bekler, F. Matpan   +8 more
core   +1 more source