Results 181 to 190 of about 600,320 (348)

Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10

FEBS Open Bio, EarlyView.
Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee, Ga Seul Lee, JinHan Kim, Kwang‐Hyun Park, Seong‐Ryung Go, Jeong Hee Moon, Eui‐Jeon Woo   +6 more
wiley   +1 more source

Top 10 plant-parasitic nematodes in molecular plant pathology.

Molecular plant pathology, 2013
John T Jones, A. Haegeman, E. Danchin, H. S. Gaur, J. Helder, Michael G. K. Jones, T. Kikuchi, R. Manzanilla-López, J. E. Palomares‐Rius, W. Wesemael, R. Perry   +10 more
semanticscholar   +1 more source

Suppression of lung adenocarcinoma migration through organelle alkalization by human lactoferrin – albumin fusion

FEBS Open Bio, EarlyView.
This paper reveals how human lactoferrin–albumin fusion (hLF‐HSA) potently suppresses lung adenocarcinoma cell migration. hLF‐HSA upregulates NHE7, leading to Golgi alkalization, disruption of the Golgi secretome, downregulation of MMP1, and reversal of EMT. These findings suggest a novel Golgi‐targeting strategy to suppress cancer cell migration.
Hana Nopia, Masahiro Kimura, Daisuke Kurimoto, Atsushi Sato   +3 more
wiley   +1 more source

Overexpression of SH2-SH2-SH3 domain of phospholipase C-gamma 1 blocks PDGF-induced inositol phosphate generation in NIH 3T3 cells

, 2019
Src homology (SH) 2 and 3 domains are known to be binding motifs for protein-protein interaction in signaling molecules. Among several PLC isozymes, only PLC-gamma contains the SH domain between the X and Y domains, which are known to have catalytic ...
Kim, JH, Chang, JS, Lee, YH, Ryu, SH, Min, DS, Suh, PG, Bae, SS   +6 more
core  

YIPFα1A expression is regulated by multilayered molecular mechanisms

FEBS Open Bio, EarlyView.
YIPFα1A, a five‐pass Golgi protein, is regulated at multiple layers. (1) Rare‐codon enrichment drives translation‐coupled mRNA decay. (2) A proximal 3′‐UTR element stabilizes mRNA. (3) A distal 3′‐UTR element included by alternate poly(A) site usage represses translation, which can be overridden by the proximal 3′‐UTR element.
Tokio Takaji, Yurika Nakanishi, Nobuhiro Nakamura   +2 more
wiley   +1 more source

Identifying transcription factors controlling the basal expression of human MRP4 highlights a substantial role for Sp1

FEBS Open Bio, EarlyView.
The MRP4 transporter exports several drugs and signaling molecules. Here, we identified key promoter elements regulating basal MRP4 expression. Using reporter assays, we defined a conserved region with essential Sp1 and contributory Ets sites, which controlled basal MRP4 expression.
Debora Singer, Angelique Kragl, Katrin Ziems, Juliane Glaubitz, Sophie Grammbauer, Susanne Hildebrandt, Mladen V. Tzvetkov, Gabriele Jedlitschky   +7 more
wiley   +1 more source

Practical guidelines for producing non-replicating canine adenovirus vectors. [PDF]

PLoS One
Omara D, Ndekezi C, Mugaba S, Nakyanzi A, Bukenya H, Bwogi J, Natwijuka F, Kapaata A, Kato F, Byamukama D, Ayebale ML, Kateete DP, Serwanga J, Cose S, Obondo SJ, Kaleebu P, Balinda SN.   +16 more
europepmc   +1 more source

Derivation and characterization of retinal pigment epithelium from urine‐derived iPSCs

FEBS Open Bio, EarlyView.
Age‐related macular degeneration causes vision loss via RPE dysfunction and loss. Traditional iPSC therapies rely on invasive biopsies, limiting scalability. Here, we utilize urine‐derived stem cells as an accessible source to generate u‐iPSCs, successfully differentiated into pigmented RPE. This “Urine‐to‐Retina” platform provides a promising path for
Daniella Beiner, Hainan Zhu, Carol Christine Bosholm, Heuy‐Ching Wang, Tracy Criswell, Anthony Atala, Jian‐Xing Ma, Yuanyuan Zhang   +7 more
wiley   +1 more source

Optimised plasmids for sustained transgene expression in vivo

, 2010
Plasmid based gene therapy approaches often lack long term transgene expression in vivo due to silencing or loss of the vector. One way to overcome these limitations is to combine non-silenced promoters with strong viral enhancers.
Magnusson, Terese
core  

Xeno-monitoring the impact of Vector Control on trypanosome transmission in the Forecariah sleeping sickness focus (Guinea). [PDF]

PLoS Negl Trop Dis
Camara AD, Boedouno P, Camara I, Soumah A, Kébé M, Koivogui J, Rotureau B, Bart JM, Bucheton B, Delamou A, Belem AMG, Kagbadouno M, Camara M.   +12 more
europepmc   +1 more source