Engineering a minimal cloning vector from a pUC18 plasmid backbone with an extended multiple cloning site [PDF]
, 2019 Minimal plasmids play an essential role in many intermediate steps in molecular biology. For example, they can be used to assemble building blocks in synthetic biology or be used as intermediate cloning plasmids that are ideal for PCR-based mutagenesis ...
Alci, Kûbra +4 more
core +2 more sources
Identification of sex determination genes and their evolution in Phlebotominae sand flies (Diptera, Nematocera) [PDF]
, 2019 Phlebotomine sand flies (Diptera, Nematocera) are important vectors of several pathogens, including Leishmania parasites, causing serious diseases of humans and dogs.
Aceto, Serena +9 more
core +2 more sources
Engineering a minimal cloning vector from a pUC18 plasmid backbone with an extended multiple cloning site [PDF]
, 2018 Minimal plasmids play an essential role in many intermediate steps in molecular biology. They can for example be used to assemble building blocks in synthetic biology or be used as intermediate cloning plasmids that are ideal for PCR-based mutagenesis ...
Beyaert, Rudi +2 more
core +1 more source
Rapid single step subcloning procedure by combined action of type II and type IIs endonucleases with ligase [PDF]
, 2007 Background The subcloning of a DNA fragment from an entry vector into a destination vector is a routinely performed task in molecular biology labs. Results We here present a novel benchtop procedure to achieve rapid recombination into any destination ...
Fromme, Tobias, Klingenspor, Martin
core +3 more sources
A non-homogeneous dynamic Bayesian network with sequentially coupled interaction parameters for applications in systems and synthetic biology [PDF]
, 2012 An important and challenging problem in systems biology is the inference of gene regulatory networks from short non-stationary time series of transcriptional profiles.
Grzegorczyk, M., Husmeier, D.
core +8 more sources
Designer diatom episomes delivered by bacterial conjugation. [PDF]
, 2015 Eukaryotic microalgae hold great promise for the bioproduction of fuels and higher value chemicals. However, compared with model genetic organisms such as Escherichia coli and Saccharomyces cerevisiae, characterization of the complex biology and ...
Allen, Andrew E +19 more
core +2 more sources
In Vitro Assembly of Multiple DNA Fragments Using Successive Hybridization [PDF]
, 2012 Construction of recombinant DNA from multiple fragments is widely required in molecular biology, especially for synthetic biology purposes. Here we describe a new method, successive hybridization assembling (SHA) which can rapidly do this in a single ...
B François +18 more
core +4 more sources
Graph Representation Learning in Biomedicine [PDF]
, 2021 Biomedical networks (or graphs) are universal descriptors for systems of interacting elements, from molecular interactions and disease co-morbidity to healthcare systems and scientific knowledge. Advances in artificial intelligence, specifically deep learning, have enabled us to model, analyze, and learn with such networked data. In this review, we put
arxiv +1 more source
Significance and transmission of maize streak virus disease in Africa and options for management: A review [PDF]
, 2010 The distribution of leafhopper vector populations and the viral diseases they vector are inherently influenced by agro-ecological factors. This review discusses the distribution and classification of MSVstrains, their vectors and how agro-ecological ...
Magenya, OEV, Mueke, J, Omwega, C
core +2 more sources
Reviewing molecular adaptations of Lyme borreliosis spirochetes in the context of reproductive fitness in natural transmission cycles [PDF]
, 2009 Lyme borreliosis (LB) is caused by a group of pathogenic spirochetes – most often Borrelia burgdorferi, B. afzelii, and B. garinii – that are vectored by hard ticks in the Ixodes ricinus-persulcatus complex, which feed on a variety of mammals, birds, and
Tsao, Jean I.
core +3 more sources