Results 41 to 50 of about 2,908,938 (258)
FEBS Open Bio, EarlyView.Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty +13 more
wiley +1 more source
Well-posed Vector Optimization Problems and Vector Variational Inequalities [PDF]
In this paper we introduce notions of well-posedness for a vector optimization problem and for a vector variational inequality of differential type, we study their basic properties and we establish the links among them.
Rocca Matteo
core
Support Vector Machines in R [PDF]
, 2005 Being among the most popular and efficient classification and regression methods currently available, implementations of support vector machines exist in almost every popular programming language.
Kurt Hornik +5 more
core +1 more source
In silico and in vitro exploration of a tyrosinase for biocatalytic production of catechols
FEBS Open Bio, EarlyView.Tyrosinase from Ralstonia pseudosolanacearum is a promising biocatalyst for producing valuable catechols from monophenol substrates. This tyrosinase is uniquely suited to this due to its high monophenolase : diphenolase ratio. We combined in silico docking and in vivo kinetic characterisation of this tyrosinase with 11 industrially relevant monophenols,
James Britton +6 more
wiley +1 more source
Well-posedness and scalarization in vector optimization [PDF]
In this paper we study several existing notions of well-posedness for vector optimization problems. We distinguish them into two classes and we establish the hierarchical structure of their relationships.
Rocca Matteo +2 more
core
FEBS Open Bio, EarlyView.We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
FEBS Open Bio, EarlyView.An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
Well-posedness in vector optimization and scalarization results [PDF]
In this paper, we give a survey on well-posedness notions of Tykhonov's type for vector optimization problems and the links between them with respect to the classification proposed by Miglierina, Molho and Rocca in [33].
Rocca Matteo, Papalia Melania
core
FEBS Open Bio, EarlyView.In this study, a novel esterase from the thermoacidophilic archaeon Thermoplasma acidophilum was biochemically and structurally characterized. Our results demonstrate that Ta0887 is a highly thermostable esterase that preferentially hydrolyzes p‐nitrophenyl hexanoate and possesses an α‐helical cap domain that likely contributes to its substrate ...
Alejandro Delgado‐Rey +4 more
wiley +1 more source
Two approaches toward constrained vector optimization and identity of the solutions [PDF]
In this paper we deal with a Fritz John type constrained vector optimization problem. In spite that there are many concepts of solutions for an unconstrained vector optimization problem, we show the possibility “to double” the number of concepts when a ...
Rocca Matteo +2 more
core